Disclosed are an apparatus and methods for rapid amplification of nucleic acids. More particularly, the present disclosure relates to an apparatus for mixing a reaction solution during amplification of nucleic acids and to methods for amplifying nucleic acids. Also disclosed are methods for lysing cells in a sample and amplifying nucleic acids.

Disclosed are an apparatus and methods for rapid amplification of nucleic acids. More particularly, the present disclosure relates to an apparatus for mixing a reaction solution during amplification of nucleic acids and to methods for amplifying nucleic acids. Also disclosed are methods for lysing cells in a sample and amplifying nucleic acids.

A cell bag rotator is constructed to slowly agitate a plurality of cell bags by rotation along a rotation axis that is at least partially horizontal. The cell bag rotator comprises a plurality of plates to which a respective cell bag is attached. The cell bag rotator is suitable for mixing cells with particles for performing various types of subsequent cell therapy processes on the cells.

A cell bag rotator is constructed to slowly agitate a plurality of cell bags by rotation along a rotation axis that is at least partially horizontal. The cell bag rotator comprises a plurality of plates to which a respective cell bag is attached. The cell bag rotator is suitable for mixing cells with particles for performing various types of subsequent cell therapy processes on the cells.

A culture bag includes a culture portion which has a culture space configured to contain and culture a culture fluid. The culture space is an endless circumferential circulation space in which the culture fluid can circumferentially circulate. The culture fluid can continue to circumferentially circulate in one direction in the endless culture space. This suppresses complexification of flow of the culture fluid. As a result, bubbles can be suppressed from generating without decreasing culture efficiency.

The present invention relates to a method capable of detecting, identifying or counting microorganisms, and a system using the same, and provides a method capable of identifying, detecting or counting microorganisms in a more rapid, accurate and convenient manner than a conventional method for identifying, detecting or counting microorganisms. According to the present invention, it was found that identification, detection or counting of microorganisms can be performed in a rapid, accurate and convenient manner, when a fluorescently labeled microorganism sample is centrifuged and attached to the surface of a slide, followed by analysis of fluorescent images. Therefore, the method and system of the present invention can be useful in various fields requiring detection, identification and counting of microorganisms.

A centrifugal structure member of a microfluidic chip and a centrifuge are provided. The centrifugal structure member of a microfluidic chip includes: a connecting portion (10) configured to connect to a rotor of a centrifuge; a support portion (20) fixedly connected or integrally formed with the connecting portion (10), and having an inclined outer surface forming an included angle with the rotation axis (50) of the rotor of the centrifuge; and a mounting portion (30) connected with or formed on the inclined outer surface, and configured to detachably mount the microfluidic chip (40) on the support portion (20).

A means capable of simply and efficiently concentrating a cell suspension. A concentrator has a culture vessel having a first port and a second port, a server bag having a port, a case having a hollow fiber bundle in the internal space, a filtering device having an inlet port, a first outlet port, and a second outlet port, a collection vessel having a port, a liquid supply circuit connected to the first port, the inlet port, and the server bag's port so that flow passages are switchable, a liquid discharge circuit connected to the second port, the first outlet port, the second outlet port, and the collection vessel's port so that flow passages are switchable, a liquid supply mechanism having a switching mechanism, a supply pump, and a discharge pump, and a rotation mechanism rotating the filtering device.

A compact culture device capable of performing large-scale culture in an aseptic state. A culture device has a culture bag which has ports through which a liquid flows and enables cell culture, a bag holding portion having holding plates holding the culture bag and rotating shafts, a rotation mechanism supporting and rotating the rotating shaft, a liquid supply/discharge mechanism supplying and discharging a liquid to/from the culture bag, and a rotation control portion controlling the rotation of the rotation mechanism. The rotation control portion brings the bag holding portion into a first position in parallel with the approximately horizontal direction according to a first information, brings the bag holding portion into a second position when a culture medium in the culture bag is caused to flow out of the port according to a second information, and brings the bag holding portion into a third position in parallel with the approximately vertical direction at least when a cell suspension in the culture bag is caused to flow out of the port according to a third information.

A compact culture device capable of performing large-scale culture in an aseptic state. A culture device has a culture bag which has ports through which a liquid flows and enables cell culture, a bag holding portion having holding plates holding the culture bag and rotating shafts, a rotation mechanism supporting and rotating the rotating shaft, a liquid supply/discharge mechanism supplying and discharging a liquid to/from the culture bag, and a rotation control portion controlling the rotation of the rotation mechanism. The rotation control portion brings the bag holding portion into a first position in parallel with the approximately horizontal direction according to a first information, brings the bag holding portion into a second position when a culture medium in the culture bag is caused to flow out of the port according to a second information, and brings the bag holding portion into a third position in parallel with the approximately vertical direction at least when a cell suspension in the culture bag is caused to flow out of the port according to a third information.