A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be asepetically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.

The present invention relates to a novel bioreactor system for cell cultivation. More specifically, the invention relates to a compact bioreactor system which has several integrated functions and enables small scale static culture as well as scale-up rocking culture in the same bioreactor. The bioreactor system comprises tray for positioning of a cell culture bag having adjustable volume, a lid covering the cell culture bag and provided with heating function, an integrated perfusion unit, an integrated cell loading unit, and an integrated unit for automatic cell culture sampling, wherein the bioreactor system is controlled by a single control unit. The invention also relates to a method of cell culture using the bioreactor system for culture of therapeutic cells.

A device for single-cell analysis according to an embodiment of the present invention comprises: a substrate; a gap between the substrate and porous membrane which is a space for culture medium; and a porous membrane formed on having a pore capable of isolating a second cell into single cell units.

A method for single-cell analysis according to an embodiment of the present invention comprises: Culturing a first cell in a culture medium on a bottom side of porous membrane; Applying a sample including a second cell on a porous membrane in a culture medium; Isolating the second cell into single cell units in a pore existing in the porous membrane with a external force such as agitation and gravitational force; Generating an interaction situation between the first cells and the single cell-level second cell; Analyzing a cellular phenomena of the first cell or the second cell.

A method of transferring a liquid between multiple containers in cell culture. A first container of multiple culture containers each having a port for transferring content, a three-way stopcock and a medium supply container having one port are connected by a tube, and one pump is arranged therebetween; when the number of containers in the multiple culture containers is two, the three-way stopcock and a second culture container are connected by a tube; when the number is three or more, a three-way stopcock is newly provided between the immediately connected culture container and the three-way stopcock, and connection of the newly provided three-way stopcock and a subsequent culture container by a tube is repeated, whereby all culture containers are connected to the three-way stopcock; and the culture medium is transferred from the medium supply container to each container in the multiple culture containers via the three-way stopcock by the pump.

Provided herein are biocompatible scaffolds engineered to convey growth stimulatory light to cells and augment their growth on the scaffolds both in vitro and in vivo. Also provide are methods of modifying biocompatible transparent waveguides to control delivery of light from the waveguide material.

A skin sample culture apparatus which has a base frame, with a skin sample receiving surface upon which at least part of the skin sample may be placed and which extends across an area defined by the shape of the frame. A securing member which is releasably connectable to the base frame and a grip which holds the skin sample under tension. The apparatus may include a tensioner to hold the sample under tension and means for introducing a fluid to the upper or lower surface of the sample.

A culture apparatus includes: an inner case having an opening in a front surface and stainless-steel shelf rests on left and right side surfaces, the inner case configured to be sterilized with hydrogen peroxide or by dry heat sterilization; and a stainless-steel shelf plate placed on the shelf rests and slid in a front-back direction, the stainless-steel shelf plate configured to be sterilized with hydrogen peroxide or by dry heat sterilization. The shelf plate has a base surface on which a culture is placed, flanges respectively formed by being bent upward, at bent portions in end portions on left and right sides of the base surface, and sliding members respectively provided to outer surfaces of the flanges, at least on a front side in a direction of movement when the shelf plate is being inserted, the sliding members made of a material different from a material of the inner case.

The present invention discloses the active substances for preventing hearing deterioration, its preparation method, the pharmaceutical composition containing the active substances, and the preparation method of the pharmaceutical composition. The preparation method of the active substances is performed by plate cultivation, flask cultivation and fermentation tank cultivation, to obtain the active substances of Hericium erinaceus mycelia in powder form. The powder of H. erinaceus mycelia is proved to have the effect of preventing hearing deterioration.

This disclosure relates to equipment utilized to manufacture chemical agents, particularly biopharmaceuticals, using Disposable Containers (DCs).