The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.

An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

Disclosed are a spheroid array and more particularly, a droplet trapping structure array capable of isolating all or selected spheroids into an isolated droplet array environment and the use thereof. The droplet-trapping structure array and the method and device for transferring spheroids using the same have the advantages of transferring droplets or spheroids with very high efficiency and very small variation between users by simply contacting two arrays. The spheroid transfer method and device enable mass-production of spheroid arrays in an isolated environment. In particular, the droplet trapping structure array and the spheroid transfer method can be useful for the treatment of spheroids with various reagents and the exchange of culture media.

A drug screening platform simulating hyperthermic intraperitoneal chemotherapy including a dielectrophoresis system, a microfluidic chip and a heating system is disclosed. The dielectrophoresis system is used to provide a dielectrophoresis force. The microfluidic chip includes a cell culture array and observation module and a drug mixing module. The cell culture array and observation module are used to arrange the cells into a three-dimensional structure through the dielectrophoresis force to construct a three-dimensional tumor microenvironment. The drug mixing module is coupled to the cell culture array and observation module and used to automatically split and mix the inputted drugs and output the drug combinations into the cell culture array and observation module. The heating system is used for real-time temperature sensing and heating control of the drug combinations on the microfluidic chip to simulate high-temperature drug environment when performing hyperthermic intraperitoneal chemotherapy on the three-dimensional tumor microenvironment.

A method for separating a suspension includes dispensing a liquid suspension into a compartment of a first bag assembly. The first bag assembly is then rotated using a centrifuge so as to at least partially separate the suspension, the first bag assembly being disposed within a first insert that is housed within a first cavity of a rotor of the centrifuge, the first insert having an annular lip portion that freely projects out of the first cavity of the rotor by a distance of at least 1 cm.

A device (30) includes a base connector (32) having an opening (33) and an impeller connector (64) coupled to the base connector (32). The impeller connector (64) has a through-passage (66) aligned with the opening (33) of the base connector (32). Further, the device (30) includes a flexible tube (34) having a first end (36) and a second end (40), where the first end (36) of the flexible tube (34) is coupled to the impeller connector (64). Furthermore, the device (30) includes a seal component (38) and an impeller (42) coupled to the second end (40) of the flexible tube (34). Additionally, the device (34) includes an enclosure (46) disposed enclosing the impeller (42), the flexible tube (34), the impeller connector (64), and the base connector (32).

A new pumpless internal circulation photobioreactor for hydrogen production, including: an outer reaction barrel and an inner reaction barrel made of transparent materials, a gas collecting device, and an air duct arranged between the inner reaction barrel and the outer reaction barrel. An outer ring-shaped reaction chamber is formed between the inner reaction barrel and the outer reaction barrel. Liquid permeation holes and air holes are arranged on the inner reaction barrel. The gas collecting device is communicated with the outer ring-shaped reaction chamber. The air duct is communicated with the interior of the inner reaction barrel and the outer ring-shaped reaction chamber. The present invention has a simple and compact structure and is easy to operate, and gas produced by the reactor can be re-introduced into the reaction solution to provide aerodynamic power for stirring the reaction solution, which realizes low energy consumption and high-efficiency hydrogen production.

In some embodiments, an engineered tissue construct (ETC) transport and containment method is provided and includes storing a bioreactor and an ETC contained therein within a transport package via an opening in the package, and sealing the opening. Some embodiments are also directed at methods for sterilizing packaging and bioreactors contained therein.

In some embodiments, an engineered tissue construct (ETC) transport and containment method is provided and includes storing a bioreactor and an ETC contained therein within a transport package via an opening in the package, and sealing the opening. Some embodiments are also directed at methods for sterilizing packaging and bioreactors contained therein.

There is provided a three-dimensional structure in which a multilayer film is three-dimensionally curved to form an interior space. The multilayer film includes a layer containing a carbon monoatomic layer substance, a support layer, and a curve induction layer that induces a curved structure, where the layer containing the carbon monoatomic layer substance is in contact with the interior space, and the support layer is positioned between the layer containing the carbon monoatomic layer substance and the curve induction layer.