Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.

A closed tubular bioreactor with clear corrugated or noncircular tubes having a constant cross-sectional area over their length, resulting in increased production.

The invention relates to a method for culturing and detecting microorganisms, comprising the steps of providing a liquid sample (S) in a barrel (10) of a device (1) for culturing and detecting microorganisms, passing the liquid sample (S) through a first filtering membrane (40) such that microorganisms contained in the liquid sample (S) are retained at a first side (41) of the first filtering membrane (40), contacting said first side (41) with a first growth medium (210) capable of supporting growth of microorganisms, incubating the first filtering membrane (40) and the first growth medium (210) at an incubation temperature, arranging a sensing surface (51) of a gas sensor (50) in fluid connection with a second side (42) of the first filtering membrane (40), detecting a metabolic gas released by the microorganisms by means of the gas sensor (50). The invention further relates to a device (1) for culturing and detecting microorganisms, comprising a barrel (10) enclosing a barrel compartment (13) for receiving a liquid sample (S), a first piston (20) which (20) is movable in said barrel (10), wherein said barrel compartment (13) is configured to be brought in fluid communication via a first filtering membrane (40) with a sensing surface (51) of a gas sensor (50) configured to detect a metabolic gas released by microorganisms, wherein the first filtering membrane (40) is configured to retain microorganisms contained in the liquid sample (S) at the first side (41) of the first filtering membrane (40). Furthermore, a sensor cartridge (4) and a kit of parts comprising the device (1) are provided.

In a first aspect, the present invention relates to a method of producing a library of microorganisms, the method comprising the steps of: a. providing a first fluid comprising at least one single cell, b. dispersing said first fluid comprising at least one single cell in a second fluid, thereby obtaining a plurality of single-layer microfluidic droplets, wherein at least one single- layer microfluidic droplet comprises at least one single cell, wherein the second fluid is immiscible with the first fluid, c. optionally, adding to said at least one single-layer microfluidic droplet a third fluid comprising a sensing compound, wherein the third fluid is miscible with said first fluid, and wherein the third fluid is immiscible with said second fluid, d. injecting said at least one single-layer microfluidic droplet optionally comprising the sensing compound into a fourth fluid, wherein said fourth fluid is immiscible with said second fluid, thereby obtaining at least one double-layer microfluidic droplet, e. dispensing said at least one double-layer microfluidic droplet into a culture medium based on the viability of the cell, f. incubating said culture medium, thereby obtaining said library. In a second aspect, the present invention relates to a system comprising: a. a first microfluidic chip for producing a plurality of single-layer microfluidic droplets wherein at least one single-layer microfluidic droplet comprises at least one single cell, b. a first microfluidic device for collecting said plurality of single-layer microfluidic droplets, c. a second device for adding a sensing compound into said at least one single-layer microfluidic droplet comprising at least one single cell, d. a second microfluidic chip for producing a double-layer microfluidic droplet, and e. a dispensing unit. In a third aspect, the present invention relates to the use of the method according to the first aspect of the present invention in a system according to the second aspect of the present invention.

The present invention relates to a process for the production of in vitro engineered tissues, also known in the art as cultured meat, cultivated meat, cell based meat, cellular meat and/or clean meat, and a device for the production of the same. The process comprises the steps of: loading sterilizable 3D scaffolds into a seeding chamber, sterilization of the scaffolds in the seeding chamber, seeding the scaffolds by loading a first volume of culture medium into the seeding chamber, wherein the first volume of culture medium has a density of cells in suspension of 5,000 to 25,000 cell/cm.sup.2, more preferably from 10,000 to 16,000 cm.sup.2 such that the scaffolds are immersed in the culture medium, leaving the scaffolds and the first volume of culture medium in the seeding chamber for a period of 2-24 hours at 18-37° C., loading a second volume of culture medium into a bioreactor, the second volume being greater than the first volume of the culture medium wherein an incubation position of one or more movable grids inside the bioreactor confines the scaffolds to the second volume of the culture medium such that they remain immersed during an incubation step, wherein the scaffolds and the second volume of culture medium are incubated in the bioreactor for a period of 10-60 days at 18-37° C. and at a pH between 6.5-8.0, more preferably 7.0-7.4. The invention also relates to a device for the production of cultured meat.

A cell culture apparatus includes: a substrate having a first surface; a pair of structures each having a wall surface intersecting the first surface, the wall surfaces facing each other; and an electrode disposed on the first surface and traversing a space between the wall surfaces, the electrode and each of the wail surfaces forming an angle other than 90 degrees.

A system includes a bioprocessing vessel formed of a material insulative to a liquid medium when present therein. At least one sensor is for sensing a parameter of the bioprocessing vessel and generating a signal indicative of the parameter. At least ne conductor is adapted to provide electrical communication between the liquid medium in the bioprocessing vessel and an external structure to achieve equipotential for reducing noise in the signal produced by the at least one sensor. Related apparatus and methods are also disclosed.

A device for growing microorganisms. The device can include a self-supporting, water-proof substrate having an inner-facing surface and an outer-facing surface; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the substrate; a microstructured surface on the inner-facing surface of the coversheet, wherein the microstructured surface comprises a plurality of microstructures; a substantially dry, gelling agent and a substantially dry, microbial growth nutrient composition disposed on the microstructured surface of the microstructured film or on a portion of the inner-facing surface of the substrate.

A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention includes: a main body comprising a culture space having one surface that is open; and a surface-modified sheet-type culture plate which is fixed to the main body to cover the opened one surface of the culture space, and thus forms a culture surface on which cells are cultured, and which forms the culture surface so that the cells can be attached to the culture surface.

A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention includes: a main body comprising a culture space having one surface that is open; and a surface-modified sheet-type culture plate which is fixed to the main body to cover the opened one surface of the culture space, and thus forms a culture surface on which cells are cultured, and which forms the culture surface so that the cells can be attached to the culture surface.