Provided is a microorganism culture sheet having an excellent efficiency for collection of environmental microorganisms and a method for measuring the environmental microorganisms with an excellent collection efficiency. Provided is a microorganism culture sheet including a base sheet, a dry culture layer formed on the base sheet, and a cover sheet covering the dry culture layer, wherein an adhesive layer and a release film are laminated on the inner surface of the cover sheet. Because the environmental microorganisms are collected using the adhesive layer, an excellent collection efficiency is provided.

A cell cultivation method includes cultivating dedifferentiated kidney cells in a state of being non-adherent to a culture vessel for a period of 5 days or longer, forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates, during a portion of the period, and thereby restoring the physiological functions of the kidney cells.

Techniques for providing in vivo environments to in vitro cells by periodically applying mechanical stimuli to in vitro cells are provided for effective studies on cell culture. A first embodiment of the present invention provides a cell culture apparatus including: a first layer including an electroactive polymer material and elongated in at least one direction by external force applied thereto; a first fixing part fixing both ends of the first layer in the direction in which the first layer is elongated, so as to maintain the elongation of the first layer; a second layer including the electroactive polymer material and spaced apart from a surface of the first layer, the second layer being elongated in at least one direction by external force applied thereto; a second fixing part fixing both ends of the second layer in the direction in which the second layer is elongated, so as to maintain the elongation of the second layer; a first electrode part applied to a region of the first layer; and a second electrode part applied to a region of the second layer, wherein the second layer is provided in such a manner that a cell to be cultured is sandwiched between the surface of the first layer and a surface of the second layer.

Techniques for providing in vivo environments to in vitro cells by periodically applying mechanical stimuli to in vitro cells are provided for effective studies on cell culture. A first embodiment of the present invention provides a cell culture apparatus including: a first layer including an electroactive polymer material and elongated in at least one direction by external force applied thereto; a first fixing part fixing both ends of the first layer in the direction in which the first layer is elongated, so as to maintain the elongation of the first layer; a second layer including the electroactive polymer material and spaced apart from a surface of the first layer, the second layer being elongated in at least one direction by external force applied thereto; a second fixing part fixing both ends of the second layer in the direction in which the second layer is elongated, so as to maintain the elongation of the second layer; a first electrode part applied to a region of the first layer; and a second electrode part applied to a region of the second layer, wherein the second layer is provided in such a manner that a cell to be cultured is sandwiched between the surface of the first layer and a surface of the second layer.

The present invention relates to the application of a three-dimensional (3D) bioreactor for T-cell expansion for immunotherapy.

The present invention relates to the application of a three-dimensional (3D) bioreactor for T-cell expansion for immunotherapy.

The present disclosure provides a detection chip, including: a micro-cavity-defining layer having a plurality of micro-pores extending through the micro-cavity-defining layer are arranged; and a ventilative liquid-resistant layer on a side of the micro-cavity-defining layer and completely covering openings on a side of the plurality of micro-pores. The ventilative liquid-resistant layer is configured to allow gas to pass therethrough and to block liquid from passing therethrough. The present disclosure also provides a method for manufacturing a detection chip and a sample introduction method for the detection chip.

A cultivation bag assembly for the cultivation of microbes, fungi and other organisms includes a bag having first and second walls joined along side edges to define a bag interior for containing a food substrate and an organism to be cultivated. At least one of the first and second walls is constructed of a layer of water-vapor permeable material to allow the passage of water vapor therethrough. A bag wall overlay layer formed from gas impermeable layer is releasably coupled to the bag. The overlay layer overlays the layer of water-vapor permeable material to prevent the passage of gases therethrough. The bag wall overlay layer has an opening with a gas filter patch covering the opening to allow the passage of oxygen and carbon dioxide gases through the gas filter patch to and from ambient air to facilitate incubation of the organism within the interior of the bag. The bag wall overlay layer is removable from the bag to allow water vapor to pass through the layer of water-vapor permeable material to ambient air facilitate drying of the bag contents after incubation is complete.

Provided herein are devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.

Provided herein are devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.