The present disclosure provides a method of preparing a high density culture of bacteria. The method provides a bioreactor comprising a fermentation tank, a perfusion system, a medium, and a cell separator; preparing an inoculum comprising a bacteria consortia with at least two bacteria species; inoculating the fermentation tank with the inoculum to form a fermentation culture; and growing the bacteria consortia until it has reached a target density of at least about 10.sup.12 CFU/mL.

The aspects of the disclosed embodiments generally relate to an apparatus which allows for the controlled addition of antifoam to the foam present in the headspace of a disposable single-use bioreactor in a reliable manner. The aspects of the disclosed embodiments also generally relate to a method of using such apparatus which allows for the controlled addition of antifoam to the foam present in the headspace of a disposable single-use bioreactor in a reliable manner. The aspects of the disclosed embodiments generally relate to antifoam systems, methods and apparatus, and more particularly, to an antifoam device operably connected to a single use biobag.

The present invention relates to the field of fermentation. In particular, the present invention relates to a method and apparatus for production of products by use of living cells or active components derived from such cells using a bioreactor having a built in gas distributor and a specific system/device for handling foam formed in the process. The invention also relates to a bioreactor comprising: a reaction chamber having a fermentation zone and a foam settling zone, wherein the reaction chamber is arranged such that the foam settling zone is spaced from or physically separated from the fermentation zone so as to reduce the effect of activity in the fermentation zone on foam in the settling zone. The separation of the fermentation zone from the activity of the fermentation zone encourages settling of the foam. The invention also provides a method of forming a bioreactor comprising: overlapping two flat, flexible sheets of material; and causing said two sheets to adhere to each other in selected areas so as to define a reaction chamber. This is a particularly cost-effective way to produce a single use, disposable reactor.

A system for enabling a wireless single use sensing sub-system by optimizing electrical power is presented. The system includes the sensing sub-system configured to be employed in a bio process environment. Further, the sensing sub-system includes a sensing unit configured to measure at least one parameter of the bio process environment. Also, the sensing sub-system includes a power source electrically coupled to the sensing unit and configured to transmit an electrical power to the sensing unit. Furthermore, the sensing sub-system includes a switch configured to electrically couple or decouple the sensing unit from the power source. In addition, the system includes a control sub-system including a first controller configured to determine at least one power control parameter based on a user-input data and a sensing sub-system data, and optimize consumption of the electrical power in the sensing sub-system, based on the power control parameter.

Provided is a device which is capable of ongoing measurement of variable foaming tendencies in a fluid system and provide a signal to regulate the feed rate of defoamer accordingly to maintain foaming at an acceptable target level. The regulation of feed can be accomplished automatically or manually.

A multisensor and a process for the production of the multisensor for a bioreactor for use in cell culture and/or in microbiology is disclosed. The multisensor comprises at least three measurement arrangements configured to measure at least three parameters, where a first of the three measurement arrangements is configured to carry out an impedance measurement and/or a capacitive measurement, and where the first measurement arrangement has at least two electrodes which comprise an electrically conductive plasti.

A bioreactor configured to contain a volume of liquid is provided. The bioreactor includes a collapsible bag able to contain the volume of liquid, a support structure surrounding and containing the collapsible bag, a first sparger connected to the collapsible bag, the first sparger having a first aperture size, and a second sparger connected to the collapsible bag, the second sparger having a second aperture size that is different from the first aperture size.

A bioprocessing apparatus includes a flexible bag having an interior volume configured to contain a fluid, and an integral fluid conduit within the flexible bioprocessing bag. The integral fluid conduit includes a panel of material joined to an interior sidewall of the flexible bag so as to define a fluid channel between the interior sidewall of the flexible bag and the panel of material. The integral fluid conduit may include a bottom outlet opening, a top outlet opening, or both. The apparatus may further include a port in a top of the flexible bag, a port in the bottom of the flexible bag, or both, wherein the integral fluid conduit is fluidly connected to the ports.

A bioreactor for culturing of microorganisms or cells comprising: a casing defining an interior space of the bioreactor, wherein said interior space is configured to receive and hold a culture medium with microbial or cellular liquid cultures; and at least one coupling element provided on the casing for coupling with a foam mitigation device for mitigation of foam built-up in the interior space of the bioreactor during use of the bioreactor for culturing of microorganisms or cells; wherein the coupling element is configured to allow ultrasonic waves generated by the foam mitigation device act upon foam built-up in the interior space of the bioreactor.

A foam identification system including a thermal imaging camera and a controller connected to the thermal imaging camera. The thermal imaging camera images a surface of a liquid in a vessel that is exposed to a headspace of the vessel. The headspace being either warmer or cooler than the liquid. The camera and the controller detect a change in temperature of the exposed surface of the liquid to identify foam on the exposed surface of the liquid.