Provided is a culture bag accommodating a culture fluid, the culture bag is capable of suppressing foaming in the culture fluid when oscillating the culture fluid to perform a culture, and performing a culture with high efficiency. The culture bag includes a culture space accommodating a culture fluid, the culture space being an endless space to allow the culture fluid to circulate therein, in which the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a first surface formed of a fine structure and a second surface formed of a structure different from that of the first structure.

An object of the present invention is to provide a method for producing a product by cell culture, which makes it possible to suppress the clogging of an exhaust filter due to bubbles generated on an upper surface of a culture solution, and a cell culture device that makes it possible to suppress the clogging of an exhaust filter due to bubbles generated on an upper surface of a culture solution. According to the present invention, there is provided a method for producing a product, which includes culturing a cell using a cell culture device to produce a product from the cell, where the cell culture device includes a culture container accommodating a cell suspension containing cells and at least one or more bubble trap containers capable of accumulating bubbles, the one or more bubble trap containers being connected to an outside of the culture container.

The aspects of the disclosed embodiments generally relate to an apparatus which allows for the controlled addition of antifoam to the foam present in the headspace of a disposable single-use bioreactor in a reliable manner. The aspects of the disclosed embodiments also generally relate to a method of using such apparatus which allows for the controlled addition of antifoam to the foam present in the headspace of a disposable single-use bioreactor in a reliable manner. The aspects of the disclosed embodiments generally relate to antifoam systems, methods and apparatus, and more particularly, to an antifoam device operably connected to a single use biobag.

A vessel configured to contain a liquid is provided. The vessel includes a collapsible bad, a reusable support structure, a sensor, and a controller. The collapsible bag is operative to contain the liquid. The reusable support structure is operative to support the collapsible bag. The sensor is operative to measure a parameter within the collapsible bag. The controller is in electronic communication with the sensor and operative to introduce an antifoaming agent into the collapsible bag based at least in part on a signal received from the sensor.

An adjustable foam sensor system is disclosed. The adjustable foam sensor system can include an adjustable foam sensor coupled to a transition member with a foam probe extending from the transition member. The adjustable foam sensor system can also include an adjustable housing that can be coupled to a container interface for interfacing with a container of a fluid processing system. The adjustable housing can be configured to be compressed or extended in response to a force, which in turn can reposition or move the adjustable foam sensor in the container. A controller can automatically adjust the adjustable foam sensor based on sensed and/or determined fluid levels, as well as control the delivery of anti-foam for reducing foam in the container.

A bioreactor configured to contain a volume of liquid is provided. The bioreactor includes a collapsible bag able to contain the volume of liquid, a first sparger connected to the collapsible bag, and a second sparger connected to the collapsible bag.

Systems for containing and manipulating fluids including systems and methods involving supported collapsible bags that may be used as reactors for performing chemical, biochemical and/or biological reactions contained therein are provided. Generally, a series of improvements and features for fluid containment systems such as gas delivery configurations, foam control systems and bag molding methods and articles for supported collapsible bag vessels and bioreactors are provided. For instance, in one aspect, fluids contained in a vessel can be sparged, e.g., such that a fluid is directed into a container of the vessel, and in some cases, the sparging can be controlled by rapidly activating or altering the degree of sparging as needed. Multiple spargers may be used in some cases. In another aspect, the vessel includes a seamless collapsible bag. In some cases, the collapsible bag may be injected, blown, or spin cast molded. In yet another aspect, the vessel includes a device which can reduce the foam produced or contained within the vessel. Sensors and/or controllers may optionally be used to monitor and/or control foaming.

Biorcactor apparatus and methods for bioreactor processing are disclosed. A bioreactor apparatus has a vessel for containing liquid media and a head space gas and a Venturi pump assembly. The Venturi pump assembly has a media outlet conduit; a media inlet conduit; a pump device, configured to draw media from the vessel through the media outlet conduit and pass it to the media inlet conduit; and a foam collector member. The foam collector member has a mouth portion disposed within the vessel at a liquid media surface position; and a narrowed throat portion. The media inlet conduit is adapted to direct pumped media through the throat portion of the foam collector member, to generate therein a region of reduced pressure for aspirating surface foam into the mouth portion of the foam collector member.

A system for enabling a wireless single use sensing sub-system by optimizing electrical power is presented. The system includes the sensing sub-system configured to be employed in a bio process environment. Further, the sensing sub-system includes a sensing unit configured to measure at least one parameter of the bio process environment. Also, the sensing sub-system includes a power source electrically coupled to the sensing unit and configured to transmit an electrical power to the sensing unit. Furthermore, the sensing sub-system includes a switch configured to electrically couple or decouple the sensing unit from the power source. In addition, the system includes a control sub-system including a first controller configured to determine at least one power control parameter based on a user-input data and a sensing sub-system data, and optimize consumption of the electrical power in the sensing sub-system, based on the power control parameter.

A device and method for detecting the level of foam in a reactor vessel monitors the intensity of the light or the movement of the light detected by a camera which monitors at least one light source positioned inside the reactor vessel or viewable through the reactor vessel.