The invention features devices and kits for capturing and culturing microorganisms (e.g., bacteria, fungi, or protists) and methods of using the devices and kits to detect microorganisms in environmental and other samples. The device includes a nutrient media having a flat growth area on which microorganisms can grow. Samples are collected by contacting the device with any environmental sample, e.g., rolling device on a work surface or exposing device to air, or by filtering a sample through a membrane. Microorganisms deposited on the membrane derive nutrients from the underlying media and grow into colonies that can then be detected using methods known in the art. The detected colonies can be imaged digitally or with film.

An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.

The present document discloses a method of processing a sample obtained from a livestock animal, comprising applying at least some of said milk to a test surface of a growth medium test plate, waiting for a time sufficient to allow microbial growth to form on said test surface, acquiring a visual spectrum image depicting at least part of the test surface, using an image capture device, and providing a computer-implemented pre-trained image classifier algorithm, said image classifier algorithm being pre-trained to determine a microorganism type based on a visible spectrum image depicting a growth pattern of a known microorganism, and applying said image to the pre-trained image classifier algorithm to determine a microorganism type based on a microorganism growth pattern visible on the image. The document also discloses a method of training an image classifier algorithm, an image capture support for use in acquiring the image, a system comprising the image capture support, a user device and a central processing device, and the use of a pre-trained image classifier algorithm for determining a microorganism type based on a visible spectrum image depicting a microorganism growth pattern on a growth medium containing test plate.

A continuous automated perfusion culture analysis system (CAPCAS) comprises one or more fluidic systems configured to operate large numbers of biodevices in parallel. Each fluidic system comprises an input reservoir plate for receiving media; a biodevice plate comprising an array of biodevices fluidically coupled to the input reservoir plate, configured such that each biodevice has independent media delivery, fluid removal, stirring, and gas control, and each biodevice is capable of continuously receiving the media from the input reservoir plate; and an output plate fluidically coupled to the biodevice plate for real-time analysis and sampling. The operations of the CAPCAS are automated and computer-controlled wirelessly. The CAPCAS can also be used for abiotic and biotic chemical synthesis processes.

The present invention relates to the use of warmers, or autonomous heat packs, for heating and maintaining a solution at a suitable temperature, for the period of time required to accomplish a chemical, biochemical or biological reaction, in particular in molecular biology or cell biology applications. Biology kits containing warmers are also part of this invention.

The invention relates to a conveying device (IO) for positioning and providing laboratory vessels (12; 12a, 12b, 12c) for nutrient media, samples, microorganisms, cell cultures, or the like for analysis, sample preparation, and/or sample manipulation at an associated apparatus (64), comprising at least one first conveying unit (32) for conveying the laboratory vessels (12) between an initial region (30) and a provision region (60), where the laboratory vessel (12) is held for the analysis or preparation. According to the invention, a plurality of conveying units is present, which perform only a translational motion of the laboratory vessel (12) along an axis, wherein the first conveying unit (32) vertically conveys the laboratory vessel (12) from the initial region (30) to a predetermined height region (50) and vice versa, and a second conveying unit (56) is provided, which horizontally conveys the laboratory vessel (12) from the height region (50) to the provision region and vice versa.

One or more light sources may apply stimuli to a colony of organisms. The stimuli may include visible and non-visible light. The stimuli, taken together, may tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from infrared or ultraviolet light. In some cases, the set of stimuli may include illuminating the entire colony of organisms with green light, illuminating only a first portion of the colony with pulsed ultraviolet light, and illuminating only a second portion of the colony with pulsed infrared light.

The invention provides a device for growing cells—referred to as a cassette. The cell culturing device includes a housing that contains a lid having an optically clear window; a fluid distribution channel; a sample injection port fluidically connected to the fluid distribution channel; a base housing a porous media pad; and a media injection port fluidically connected to the media pad. The lid mates to the base to form a sterile seal; the fluid distribution channel is disposed over the media pad, which is viewable through the optical window; and sample fluid introduced into the fluid distribution channel is distributed evenly to the media pad, e.g., via a plurality of channels. The invention also provides kits that include cassettes of the invention and a tube set.

A cell culture system or assembly, flask, plate and dish for growing, viewing and evaluating cells; and methods of use.

A columnar cell culture vessel is disclosed. The columnar cell culture vessel according to an embodiment of the present disclosure may include: a cylindrical body having a chamber disposed therein, an inlet disposed through one side in an axial direction, and an outlet disposed through in the axial direction; a first cap coupled to the inlet and having an inflow port disposed therethrough; a second cap coupled to the outlet and having a discharge port disposed therethrough; and a support which is filled in the chamber and to which cells are attached to be cultured, wherein a culture solution is introduced into the chamber through the inflow port and discharged to the outside of the chamber through the discharge port.