One aspect of the invention provides a method of generating three-dimensional biological structures. The method includes: (a) depositing a first layer of a suspension over a substrate, the suspension including a liquid and a plurality of cells; (b) allowing the plurality of cells to attach to the substrate and form a first layer of attached cells; (c) depositing a cell-attachment agent over the first layer of attached cells; and (d) depositing a second layer of the suspension over the cell-attachment agent.

A filter apparatus is disclosed for withdrawing a fluid medium from a bioreactor during the growth of a cell culture within the bioreactor. Also disclosed is a method for culturing cells in a bioreactor. The filter apparatus includes a hollow tubular member attached to a filter member. The filter member has a pore size and volume capable of withdrawing a fluid medium at a relatively high flow rate from the bioreactor. Without withdrawing biological cells from the bioreactor and without damaging or harming the cells. The filter apparatus of the present disclosure allows for many process improvements.

The present invention relates to methods and systems for performing perfusion cell culture whereby the supernatant of the bleed stream is recovered.

The present invention relates to a method for producing compressed collagen comprising the steps of a) pressing a gelled collagen in a first pressing direction; and b) pressing the gelled collagen of step a) in a second pressing direction substantially orthogonal to the first pressing direction, wherein the pressing in step a) is carried out at a pressure in the range of 0.01 to 0.05 bar and in step b) at a pressure in the range of 0.5 bar to 2 bar.

Disclosed is an apparatus for the production of tissue from cells. The apparatus comprises an elongate body having at least one circumferential groove and being operable to extend, by close-fitting relationship, centrally through at least one trough. The troughs are extending in a closed path, such that the at least one of the circumferential grooves open into an inner edge of a trough. Also disclosed is a process for production of tissue from cells, via a transitioning intermediate which transitions from the cells into the tissue.

A bioreactor for producing hydrogen gas and other metabolites. The bioreactor utilizes light, fermentation, and other metabolic processes for the production of metabolites, derived from various microorganisms contained within the bioreactor through respective metabolic pathways. The bioreactor comprises a main reactor chamber, a semipermeable membrane, a sleeve, a power supply, a substrate medium, a heating member, a plurality of tubing members, a collection reservoir, a pressure-sealed connecter member, and an agitator.

A manual injector for cell manipulation comprising a displacement device for displacing a fluid, an injection tube connected to the displacement device, and a capillary holder connected to the injection tube for holding a microcapillary tube or a microcapillary tube connected to the injection tube, wherein the displacement device has the following: a disk-shaped main body that, on a first end face, has an hollow cylinder space which is concentric with its middle axis, a disk-shaped adjusting wheel, a helical gear, a hollow cylindrical plunger, a rotational decoupling that connects the adjusting wheel to the plunger, and a connection connected to the cylinder space to which the injection tube is connected.

The present disclosure relates to a 3D bioprinter (1) comprising a base unit (2). The base unit (2) has a support (3) adapted for mounting of at least one toolhead (4), a communication interface part (5) for communication of data with the at least one toolhead (4), when mounted, and a base unit processing element (7) adapted to communicate with a toolhead processing element (8) of the at least one toolhead over said communication interface part (5). The present disclosure relates further to a 3D bioprinter toolhead. The present disclosure relates further to a method for bioprinting a construct.

There is provided a connector, for introducing or extracting a material to or from at least one receptacle, comprising a housing extending between a distal end and a proximal end, the housing comprising, at least at one end, a pierceable seal; a hollow needle mounted, at least partially, within the housing between the distal end and the proximal end of the housing, a first end of the hollow needle being connected or connectable to a first corresponding receptacle, and a second end of the hollow needle facing the pierceable seal at an end of the housing; and an actuating mechanism acting on the housing or the hollow needle to enable the hollow needle to pierce the pierceable seal thereby forming a communication through the pierceable seal, such that material is able to transfer through the connector.

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.