Provided is a cell wall or cell membrane disrupting device whereby cell walls and/or cell membranes of microorganisms, algae and the like contained in organic sludge and the like are disrupted, the device comprising a fixed disc, a rotating disc, a rotating shaft for driving of the rotating disc, a pressure reducing means and a housing, wherein at least one pair of the fixed disc and rotating disc are disposed facing each other, the center section of the fixed disc has a hollow section that is larger than the outer diameter of the rotating shaft passing through the center section, shearing force generated between the rotating disc and the fixed disc is applied to a target fluid having a water content of 89% or higher that has been loaded into the device, and the pressure inside the cell wall or cell membrane disrupting device is reduced to no greater than −0.08 MPa by the pressure reducing means. The device can contribute to increasing biogas, reducing sludge, culturing of algae, plant cultivation and culturing of marine products, and also to separation of CH.sub.4 and CO.sub.2, for example, as resources.

Provided are techniques that may include a disposable magnetic cell isolation holder having one or more passages that accommodate a magnetic retention material to facilitate magnetic cell isolation without adjustment of magnetic field parameters between isolation procedures using different magnetic particle sizes. When the magnetic cell isolation holder is coupled to a magnetic field generator, a first passage corresponding to a smaller particle size is positioned in a magnetic field where the magnetic field characteristics are different relative to a second passage, of a second holder or in the same holder, for use with another bead size.

Disclosed is a device (100, 200) for the disaggregation of tissue samples into individual cells or cell clumps in a closed flexible tissue sample bag (10); the device including two or more resilient feet (134/136, 234/236) which tread sequentially a tissue sample bag receiving area (148,248). Also disclosed is a heat transfer plate (150, 250) for transferring heat energy to or from the area (148,248), the plate having one plate surface (151,251) adjacent the area (148,248) and an opposing surface (152,252) exposed to external thermal influence which faces away from the area (148,248). Further disclosed is a tissue sample receiving bag (10) comprising one or more flexible plastics cavity (12) formed from two layers of the plastics sealed around their edges to form a generally rectilinear periphery with the cavity or cavities (12) within the periphery, and at one side of the periphery is formed one or more sealable access ports (16). One part of the bag is left unsealed to provide a tissue sample receiving opening.

A cell clump dispersing device for dispersing cell clumps is provided, including a dispersion structure module that includes at least one structure for providing fluid flow shear force. Each structure for providing liquid flow shear force includes an inlet for cell clumps to be dispersed to enter the structure, and at least one outlet for dispersed cell clumps to flow out of the structure. A diameter of the outlet is smaller than that of the inlet, and an end of at least one of the structure is capable of being connected to an end of another of the structure.

The present invention provides a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), methods of using the device to generate a stromal vascular fraction (“SVF”) and methods of the SVF.

Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.

Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.

The invention relates to a method and a biogas plant for producing biogas, preferably from rice straw, wherein a substrate is fermented in two reactors (1, 2) in a circulating manner, so that a methane production from cellulose-and/or lignocellulose-containing substrate can be improved.

The present invention provides a stem cell manufacturing system comprising: a pre-transfer cell solution sending channel 20 through which a solution containing cells flows; an inducer solution sending mechanism 21 which sends a pluripotency inducer into the pre-transfer cell solution sending channel 20; an inducer transfer apparatus 30 which is connected to the pre-transfer cell solution sending channel 20 and transfers the pluripotency inducer into the cells to produce cells harboring the inducer; a cell cluster production apparatus 40 which cultures the cells harboring the inducer to produce a plurality of cell clusters consisting of stem cells; and a packaging apparatus 100 which sequentially packages the plurality of cell clusters.

A trituration device for tissue disaggregation includes a device housing having a hollow cylinder defining an opening extending from a first end of the device housing to a second end of the device housing, a rotor block positioned in the opening of the device housing, and a trituration grater positioned within the opening of the device housing to contact the rotor block. The rotor block includes a solid cylinder having a central channel and at least one surface that defines a rotor blade, and the trituration grater includes multiple angled cutting holes. The device also includes a collection vessel positioned at the second end of the device housing to collect disaggregated tissue of a tissue specimen, and an actuating stem extending through the central channel of the rotor block to rotate the rotor block in response to rotation of the actuating stem.