The present invention provides cell assay systems and its use in cell based assays.

A cell culture evaluation device includes at least one processor. The processor is configured to acquire a cell image obtained by imaging a cell that is being cultured, to input the cell image to an image machine learning model and output an image feature amount set composed of a plurality of types of image feature amounts related to the cell image from the image machine learning model; and to input the image feature amount set to a data machine learning model and output an expression level set composed of expression levels of a plurality of types of ribonucleic acids of the cell from the data machine learning model.

A bioreactor cleaning system for cleaning a bioreactor in a rail vehicle. A suction unit, a supply unit, an electronic control unit for actuating the suction unit and the supply unit, an acid tank, a collection tank for receiving a liquid suctioned out of the bioreactor, and a freshwater connection are provided. The bioreactor cleaning system comprises a metering unit which has acid canister connections, base canister connections, an acid metering device which can be connected to the acid canister connections and the acid tank, and a base metering device which can be connected to the base canister connections and the acid tank. Additionally, a mixer is provided in order to mix the different liquids.

In a configuration for analyzing data of cells measured by a cell measuring apparatus, accuracy of cell classification is improved without requiring the cell measuring apparatus to have high information processing capability. A cell analysis method, using a cell analyzer for analyzing cells in accordance with an artificial intelligence algorithm, includes: obtaining the data regarding the cells measured by the cell measuring apparatus; analyzing the data to generate information regarding a cell type of each of the cells; and transmitting the information to the cell measuring apparatus.

A probe holder (1), for positioning at least one probe (50) such that the probe (50) at least partially engages in a system (100) for biotechnological uses, comprises a holder base (10) for positioning the probe holder (1) on the system (100) for biotechnological uses and at least one probe positioning means (20), which is designed to position the at least one probe (50) such that the probe (50) extends in a probe extension direction (S; S1, S2, S3, S4). The probe positioning means (20) is positioned on the holder base (10) such that the position of the probe (50) is variably adjustable in a probe extension direction (S; S1, S2, S3, S4).

The present disclosure aims to provide a manufacturing method of a cell structure. The manufacturing method comprises producing a coated region in which a culturing surface is coated with a temperature-responsive polymer or a temperature-responsive polymer composition, forming a droplet of a cell suspension in the coated region, and performing cell culturing in the droplet. A surface zeta potential of the coated region is 0 mV to 50 mV.

A method of performing antimicrobial susceptibility testing (AST) on a sample uses a reader device that mounts on a mobile phone having a camera. A microtiter plate containing wells preloaded with the bacteria-containing sample, growth medium, and drugs of differing concentrations is loaded into the reader device. The wells are illuminated using an array of illumination sources contained in the reader device. Images of the wells are acquired with the camera of the mobile phone. In one embodiment, the images are transmitted to a separate computing device for processing to classify each well as turbid or not turbid and generating MIC values and a susceptibility characterization for each drug in the panel based on the turbidity classification of the array of wells. The MIC values and the susceptibility characterizations for each drug are transmitted or returned to the mobile phone for display thereon.

An integrated microfluidic chip and a single-cell culture, screening, and export method applying the same are disclosed; the chip includes a base, an inlet flow channel, an outlet flow channel, a plurality of common flow channels and a plurality of functional units, wherein two ends of the common flow channel are connected to the inlet flow channel and the outlet flow channel, respectively, wherein each of the functional units includes a single-cell introduction port, a cell culturing-screening chamber, a cell export chamber, a cell export port, and a drive element, wherein the drive element is used to provide power to liquid to introduce single cells entering the common flow channels into the cell culturing-screening chamber, and after culturing and screening, to export target cell population in the cell culturing-screening chamber through the cell export port.

The present invention is related to a biochip and production method thereof. The biochip comprises a carrier, a cell or tissue culture area deposited on the carrier, and a sensor area deposited on the carrier adjacent and fluidly communicating with the cell or tissue culture area. A containing space is contained in the cell or tissue culture area comprising a simulated vascular channel, a cell or a tissue and a culture medium. At least one sensor fixation area is contained at the sensor area for placing a sensor element. The present invention can be a model for stimulating cancer of specific patient to realtimely reflecting the cancer formation, transferring status and treatment strategies. The biochip could also carry testing drugs to observe how the drugs functioning to the cells/tissue as to provide a more accurate instruction of the drugs. The present invention can perform multiple test just within on chip which can save cost and also provide a more accurate test model for the patient.

A bioreactor system (1) has a pre-sterilized single-use bioreactor (2) with a reactor wall (20) surrounding an interior chamber (21) that receives a fluid medium (M). A sensor (3) for detecting an analyte in the medium has a sensor housing (30) with an outside thread (31) and a sensor shaft (32), a distal end portion (33) of which has an end face (34) with a region (35) permeable to the analyte. A sensor receptacle (4) connected to the reactor wall receives the sensor, to maintain the sterility of the single-use bioreactor. A circumferential flange (40) fixes the sensor receptacle to the reactor wall. The flange has an inside thread (42) that receives the outside thread. A wall (43) of the sensor receptacle is connected to the flange, and together with the sensor shaft, protrudes into the interior chamber, separating the sensor from the interior chamber.