Disclosed is a cell culture system comprising a first cell culture bioreactor system (10) for culturing cells to a predetermined cell density or quantity, the system including a bioreactor volume (20), a process controller (30), process control devices (32) which provide inputs (16) for the culture volume and culture parameter measurement devices (14), wherein the process controller is operable according to plural control program steps to control the process devices to provide inputs for a suitable cell culture environment in the bioreactor volume, and is further operable according to control program steps modified by feedback values from the culture parameter measurement devices, and comprises a memory (36) operable to record data indicative of the progress of the control program steps. Failure of the system can be rectified by moving the bioreactor volume to another similar system which has access to data indicative of any incomplete program steps which steps may have been modified by feedback from the measurement devices.

A process is provided for isolating and analyzing microorganisms contained in a sample by collecting a determined volume in a sample, the determined volume representing all or part of this sample, likely to contain at least one microorganism. The collected volume is then split up into a plurality of compartments having a culture medium, the volume of each compartment being smaller than 10 μL, each compartment being isolated from the other compartments and having no interface with the ambient atmosphere. At least one microorganism is incubated in the compartments for determined durations, and compartments are detected that contain at least one microorganism. The incubation may be extended after detecting compartments so that at least one microorganism having a defined quantity can be detected, and then the content of the detected compartments can be recovered. Finally, at least one functional parameter relative to a microorganism in the compartments is determined.

A movable cell incubator contains: a body, a first lid, a second lid and an electric control unit. The body includes a first internal space, a refrigeration room, and an airtight culture room. The first lid airtightly covers the culture room, the second lid airtightly covers the refrigeration room, and the control unit includes a microprocessor, a power module, a digital/analog conversion module defined between a microprocessor and the power module, a heating module controlling temperature of the culture room, a cooling module supplying cold source to the refrigeration room, a peristaltic pump module, a flow sensing module, a CO2 detective supply module supplying CO2 to the culture room, and a setting display module exposing and fixed on the first lid, with the peristaltic pump module aseptically connected between cell culture media and cell culture bag by multiple conveying tubes.

The technology is provided to effectively visualize culture statuses related to a plurality of culture targets. Provided is a control device including a display control unit that controls dynamic display related to a culture status of a culture target including a cell having a division potential, the culture status being estimated along a time series by morphological analysis using a learned model generated on the basis of a machine learning algorithm, in which the display control unit controls comparative display of the culture statuses of a plurality of the culture targets. Furthermore, provided is a control method including controlling, by a processor, dynamic display related to a culture status of a culture target including a cell having a division potential, the culture status being estimated along a time series by morphological analysis using a learned model generated on the basis of a machine learning algorithm, and controlling the display further including controlling comparative display of the culture statuses of a plurality of the culture targets.

A method for automated microbial monitoring in an isolator having a transfer lock, the method comprising the steps: first providing at least one nutrient medium carrier holder at, in each case, a first position within the isolator; second providing at least one nutrient medium carrier within the transfer lock; first robot-assisted transferring of an individual nutrient medium carrier from the transfer lock to a free nutrient medium carrier holder; and first robot-assisted placing of the transferred nutrient medium carrier in the free nutrient medium carrier holder. Further, a system for automated microbial monitoring in an isolator and a computer program are also disclosed.

An in vitro endothelial cell culture system for optimizing the pulsatile working mode of a continuous flow artificial heart belongs to the technical field of artificial organs. The system includes three parts: 1) a cell culture model on a microfluidic chip and an off-chip multielement aortic arch afterload fluid mechanics circulation loop; 2) devices for simulating the power source of a cardiovascular system: a fluid loading device is realized by a pulse blood pump, and an artificial heart device is connected in parallel to both ends of the pulse blood pump; and 3) a peripheral detection and feedback control system, comprising pressure and flow sensors, a fluorescence microscope, a CCD high-speed camera system and a proportional-integral-derivative feedback control system. The system can accurately simulate the real hemodynamics microenvironment of vascular endothelial cells in different parts of the aortic arch.

Provided are a cell culture process search method, a cell culture process search program, and a cell culture process search device capable of searching for an optimal culture condition. A cell culture process search method includes a process condition generation step of generating a plurality of process conditions for culturing a cell, a culture result prediction step of acquiring a culture prediction result of the cell for each of the plurality of process conditions generated in the process condition generation step, and an optimized process condition acquisition step of finding out an optimal process condition from the culture prediction result obtained in the culture result prediction step. A cell culture process search program to be executed and a cell culture process search device are provided. The process condition includes at least either of a plurality of culture medium compositions or a plurality of culture conditions.

Disclosed herein is an instrument suitable for processing cells for example culturing, concentrating or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump; and a disposable processing kit complementary to the mechanical elements within the housing and comprising a fluid circuit including a fluid reservoir and plural fluid paths capable of carrying fluid flow caused by said pump(s), the instrument further including a mechanism for determining the quantity, or change in quantity of the fluid in the reservoir resulting from said fluid flow, the instrument yet further comprising a controller operable to control at least the pump and operable to perform a fault determination process, which includes the steps of determining the expected flow rate of said pump(s) calculated from the speed of the pump(s) and comparing that expected flow with the change in quantity of the fluid in the reservoir as determined by said mechanism.

A system for an automated production of high cell density seed culture, HCDC, preparations, for cryo-preservation comprising a cell expansion device for growing a HCDC in a medium inside a device volume of the cell expansion device, at least one sensor for collecting data by at least temporally monitoring at least one parameter being indicative of the physiological state of the HCDC; and a control unit for processing the collected data to determine whether at least one predetermined criterion is fulfilled by the monitored parameter to control, based on whether the at least one predetermined criterion is fulfilled by the monitored parameter, at least a partial exchange of the medium by a cryo-protective agent, CPA.

Systems (100) and methods for perfusion control in a bioreactor (120) are provided. The system (100) comprises a media container (110) connected to the bioreactor (120) and weighing scales (113, 123) measure weight of the bioreactor (120) and the media container (110). A plurality of controllers (225, 245) are connected to the weighing scales and configured to continuously feed the media from media container (110) to the bioreactor (120) at a user defined rate using a motor pump (112). A filter (130) is provided to receive feed from the bioreactor (120) through a recirculation line (121) and a permeate flow line (141) is connected to the filter (130) to flow out the permeate from the filter (130). When weight of the bioreactor (120) goes beyond the upper (U) or lower (L) permissible weight limit, a controller (225) connected to the weighing scale of the bioreactor (120) sends a signal to operate the permeate motor pump (142) either to flow out the permeate from the filter (130) or to stop the motor pump (142).