An apparatus comprising an actuator for moving an actuator rod and a bioreactor vessel is disclosed herein. The bioreactor vessel comprises a container for holding a liquid, a mounting for mounting a tissue sample in the container, and, an actuator coupling to enable the actuator rod to be connected for applying mechanical force to the tissue sample. The apparatus also comprises a seat, fixed with respect to the actuator and configured for locating the reactor vessel in a location selected so that the actuator can be connected for applying said force via the actuator coupling. The reactor vessel is removable from the apparatus.

The present disclosure provides for methods of delivering cargo material to a cell(s) in vivo or in vitro, devices configured to deliver cargo material to the cell, systems configured to deliver cargo material to the cell, and the like. The present disclosure can synergistically combine multiple technologies to improve the rate of cargo material introduction and utility of physical cellular modification in a transformative way compared to the current microinjection state-of-the art.

In accordance with the present disclosure, exposure of a sample to one or more electric pulses via capacitive coupling is described. In certain embodiments, the sample may be a biological sample to be treated or modified using the pulsed electric fields. In certain embodiments, the electric pulses may be delivered to a load using capacitive coupling. In other embodiments, the electric pulses may be bipolar pulses.

The present invention relates to an operating liquid for Piezo-mediated microinjection. In particular, the present invention relates to use of purified perfluoron-octane or a composition comprising purified perfluoro-n-octane as operating liquid for Piezo-mediated intracytoplasmic sperm injection (Piezo-ICSI). The present invention further relates to methods of fertilising an oocyte in vitro by Piezo-ICSI using purified perfluoro-n-octane or a composition comprising purified perfluoro-noctane as operating liquid and methods of assisted reproduction using methods of the invention.

Described herein are nano straw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane from a fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., >70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.

An in vitro endothelial cell culture system for optimizing the pulsatile working mode of a continuous flow artificial heart belongs to the technical field of artificial organs. The system includes three parts: 1) a cell culture model on a microfluidic chip and an off-chip multielement aortic arch afterload fluid mechanics circulation loop; 2) devices for simulating the power source of a cardiovascular system: a fluid loading device is realized by a pulse blood pump, and an artificial heart device is connected in parallel to both ends of the pulse blood pump; and 3) a peripheral detection and feedback control system, comprising pressure and flow sensors, a fluorescence microscope, a CCD high-speed camera system and a proportional-integral-derivative feedback control system. The system can accurately simulate the real hemodynamics microenvironment of vascular endothelial cells in different parts of the aortic arch.

A manual injector for cell manipulation comprising a displacement device for displacing a fluid, an injection tube connected to the displacement device, and a capillary holder connected to the injection tube for holding a microcapillary tube or a microcapillary tube connected to the injection tube, wherein the displacement device has the following: a disk-shaped main body that, on a first end face, has an hollow cylinder space which is concentric with its middle axis, a disk-shaped adjusting wheel, a helical gear, a hollow cylindrical plunger, a rotational decoupling that connects the adjusting wheel to the plunger, and a connection connected to the cylinder space to which the injection tube is connected.

The invented bio-electrical system is a housing-electrode which allows insertion of another electrode for various electrochemical and bio-electrical applications. Together with other invented elements as well as standard components, the system is fully scalable, modular, and allows production and collection of gases under pressure. It can be built in many shapes, such as the embodied tubular shape. The design allows operation on unstable ground, for example on ships. Flow of electrolyte can be regulated and directed in cascaded reactions by opening and closing the compartments of the outer or the inner electrodes using the provided electrode holders. The redox conditions inside the system can be controlled using off-the-shelf power supplies which are controlled using the provided algorithm. Gas collection can be regulated based on the level of liquid inside the system using the provided float switches or conductivity probes even as the system is moving or operated under zero-gravity conditions.

Disclosed herein are methods of inducing endothelial cell reorganization or differentiation to form micro- and macrovessel structures using an extracellular matrix, such as one derived from small mobile stem (SMS) cells, and a substrate, which can also be coated in molecules or otherwise physically manipulated to cause localized effects on reorganization Also disclosed is a kit implementation for performing endothelial cell reorganization.

A substance introduction device according to the present disclosure is a substance introduction device that introduces the substance into the cell by the electroporation, the substance introduction device including: a storage unit that stores the cell suspension containing the cells and the substance; an introduction path that introduces the cell suspension into the storage unit; a filling unit that fills the storage unit with the predetermined amount of the cell suspension from the introduction path; an electrode pair that applies a voltage to the cell suspension stored in the storage unit; and a discharge path that discharges the cell suspension after the voltage is applied from the storage unit.