An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm.sup.1 ) defined by the quotient of the electrode gap squared (cm.sup.2) divided by the chamber volume (cm.sup.3), wherein the geometric factor is less than or equal to 0.1 cm.sup.1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 50 microSiemens/cm to 500 microSiemens/cm, wherein the suspension is enclosed in the chamber during treatment, and b. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform.

The present invention relates to a culture device for tissue cell suspension. The culture device includes a tissue cell culture body. The tissue cell culture body is a porous material. The porous material is formed by cavities classified into different levels according to the pore size of material and cavity walls surrounding to form the cavities. A lower level of small cavities is provided to surround the cavity wall which forms the upper level of large cavity. Cavities of each level are in communication with each other, and cavities between respective levels are also in communication with each other. The culture device further includes a swirler device provided therein. Such culture device can particularly facilitate normal and unrestricted growth of the suspension of cells in three-dimensional space, obstructing the formation of over-dense cell region or nutrient-rich region during the cell culturing.

The present invention relates to a culture device for tissue cell suspension. The culture device includes a tissue cell culture body. The tissue cell culture body is a porous material. The porous material is formed by cavities classified into different levels according to the pore size of material and cavity walls surrounding to form the cavities. A lower level of small cavities is provided to surround the cavity wall which forms the upper level of large cavity. Cavities of each level are in communication with each other, and cavities between respective levels are also in communication with each other. The culture device further includes a swirler device provided therein. Such culture device can particularly facilitate normal and unrestricted growth of the suspension of cells in three-dimensional space, obstructing the formation of over-dense cell region or nutrient-rich region during the cell culturing.

A means capable of simply and efficiently concentrating a cell suspension. A concentrator has a culture vessel having a first port and a second port, a server bag having a port, a case having a hollow fiber bundle in the internal space, a filtering device having an inlet port, a first outlet port, and a second outlet port, a collection vessel having a port, a liquid supply circuit connected to the first port, the inlet port, and the server bag's port so that flow passages are switchable, a liquid discharge circuit connected to the second port, the first outlet port, the second outlet port, and the collection vessel's port so that flow passages are switchable, a liquid supply mechanism having a switching mechanism, a supply pump, and a discharge pump, and a rotation mechanism rotating the filtering device.

An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm.sup.1) defined by the quotient of the electrode gap squared (cm.sup.2) divided by the chamber volume (cm.sup.3), wherein the geometric factor is less than or equal to 0.1 cm.sup.1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 50 microSiemens/cm to 500 microSiemens/cm, wherein the suspension is enclosed in the chamber during treatment, and b. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform.

A compact culture device capable of performing large-scale culture in an aseptic state. A culture device has a culture bag which has ports through which a liquid flows and enables cell culture, a bag holding portion having holding plates holding the culture bag and rotating shafts, a rotation mechanism supporting and rotating the rotating shaft, a liquid supply/discharge mechanism supplying and discharging a liquid to/from the culture bag, and a rotation control portion controlling the rotation of the rotation mechanism. The rotation control portion brings the bag holding portion into a first position in parallel with the approximately horizontal direction according to a first information, brings the bag holding portion into a second position when a culture medium in the culture bag is caused to flow out of the port according to a second information, and brings the bag holding portion into a third position in parallel with the approximately vertical direction at least when a cell suspension in the culture bag is caused to flow out of the port according to a third information.

A compact culture device capable of performing large-scale culture in an aseptic state. A culture device has a culture bag which has ports through which a liquid flows and enables cell culture, a bag holding portion having holding plates holding the culture bag and rotating shafts, a rotation mechanism supporting and rotating the rotating shaft, a liquid supply/discharge mechanism supplying and discharging a liquid to/from the culture bag, and a rotation control portion controlling the rotation of the rotation mechanism. The rotation control portion brings the bag holding portion into a first position in parallel with the approximately horizontal direction according to a first information, brings the bag holding portion into a second position when a culture medium in the culture bag is caused to flow out of the port according to a second information, and brings the bag holding portion into a third position in parallel with the approximately vertical direction at least when a cell suspension in the culture bag is caused to flow out of the port according to a third information.

In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.

Provided is an apparatus to produce cultured cell products including: an isolator configured to maintain its inside in aseptic conditions and process cell culture vessels therein; and at least one robot arm located within the isolator, wherein a taking-out step of taking out cells cultured in the cell culture vessels, a cell density-adjusting step of adjusting density of the cells in a cell-containing liquid containing the taken-out cells, and a subdividing step of subdividing and placing the cell-containing liquid with its density adjusted into a plurality of product containers are performed within the isolator by the at least one robot arm.

Provided is an apparatus to produce cultured cell products including: an isolator configured to maintain its inside in aseptic conditions and process cell culture vessels therein; and at least one robot arm located within the isolator, wherein a taking-out step of taking out cells cultured in the cell culture vessels, a cell density-adjusting step of adjusting density of the cells in a cell-containing liquid containing the taken-out cells, and a subdividing step of subdividing and placing the cell-containing liquid with its density adjusted into a plurality of product containers are performed within the isolator by the at least one robot arm.