A cylindrical flow distributor (100) for performing, by means of solid reaction members, a biological or chemical transformation, or physical or chemical trapping from, or release of agents to, a fluidic media is provided. The flow distributor (100) comprises: a top wall (1); a bottom wall (2) comprising a central through going opening (13); and an outer wall (3) extending between the top wall (1) and the bottom wall (2). The top wall (1), the bottom wall (2) and an inner envelope surface (5) of said outer wall (3) together define a confinement (7) configured to contain solid reaction membersor a rigid body of a reaction member material. The outer wall (3) comprises a first plurality of longitudinally extending ribs (20) arranged side by side with longitudinal gaps (21) extending in the circumferential direction between two adjacent ribs (20), and a circumferentially extending first scaffold (22) encircling and being fixedly attached to a peripheral outer surface (29) of said plurality of longitudinally extending ribs (20). Further, a reactor using such flow distributor (100) is provided.

This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

Methods and systems for processing suspensions of biological cells and microcarriers are disclosed. The biological cells are separated from the microcarriers by introducing the suspension into a spinning membrane separator whereby the biological cells pass through the membrane and the microcarriers do not pass through the membrane.

The present disclosure suggests a food waste treatment apparatus. A food waste treatment apparatus in accordance with an exemplary embodiment of the present disclosure includes: a housing including a food inlet opening at its top surface; an inner chamber where food introduced through the food inlet opening is collected; a stirring unit provided within the inner chamber and configured to stir food waste; an outer chamber positioned outside the inner chamber; and a rotation driving unit configured to rotate the stirring unit and the inner chamber, and the inner chamber and the stirring unit are rotated by the rotation driving unit in opposite directions.

An apparatus and method for preparing and culturing cells comprises a gas permeable chamber having at least an inlet and an outlet for introducing culture medium and cells. A cell growth substrate placed at the end(s) of the gas permeable chamber for cells anchored or embedded. The present invention discloses a simple but efficient cell culture method by slowly rotating the cell culture apparatus horizontally combining with a partially filled culture media in the gas permeable container in order to be able to expose the carriers and submerge the carriers intermittently, to achieve an optimal cell culture environment through the efficient oxygen transfer, CO2 balance, with sufficient nutrient supply, without vigorous agitation to achieve oxygen transfer. The horizontally rotation movement with the present cell growth apparatus efficient the nutrition, carbon dioxide and oxygen transfer during cultured process for production of cellular protein products, viruses or harvesting the cells.

An in situ probe, comprising reusable and disposable components, can be employed to measure cell viability in a rocking bioreactor.

Described herein are methods and systems for cell processing or, more specifically, for introducing various payloads into cells. These methods and systems use a mechanoporation approach in which cells are rapidly compressed and then released to relax while absorbing the payload. More specifically, these methods and systems enable high-throughput mechanoporation with various clogging mitigation features. A cell processing apparatus comprises a shell with an inner shell cylindrical surface, a core with an outer core cylindrical surface, and ridges, supported on and protruding away from one of the inner shell cylindrical surface and the outer core cylindrical surface. The core is disposed inside the shell. The outer core cylindrical surface is concentric with the inner shell cylindrical surface. Each of the ridges forms a ridge gap with the other one of the inner shell cylindrical surface and the outer core cylindrical surface.

Described herein is a modular continuous flow bioreactor for various cells. In one embodiment, the modular cell culture bioreactor apparatus may comprise a plurality of cell chambers disposed between an upper flow chamber and a lower flow chamber; a plurality of lower conduits fluidly connecting the lower flow chamber with one or more lower reservoirs and a plurality of upper conduits fluidly connecting the upper flow chamber with one or more upper reservoirs; one or more pumps fluidly connected through the plurality of conduits with the one or more reservoirs and with the upper and lower flow chambers; wherein each individual cell chamber comprises a lower permeable membrane in fluid communication with the lower flow chamber, a three-dimensional distribution of cells, and an upper permeable membrane in fluid communication with the upper flow chamber.

Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.

Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.