A method of dissociating single cells from a biological tissue sample is described herein, along with systems for performing such methods. The method includes generating one or more ultrasonic wave pulses using a transducer comprising one or more Fresnel Annular Sector Actuator (FASA) elements; and applying energy from the generated one or more ultrasonic wave pulses to a sample container holding the biological tissue sample through a coupling medium that couples the one or more FASA elements to the sample container to dissociate single cells from the biological tissue is described herein.

A method of dissociating single cells from a biological tissue sample is described herein, along with systems for performing such methods. The method includes generating one or more ultrasonic wave pulses using a transducer comprising one or more Fresnel Annular Sector Actuator (FASA) elements; and applying energy from the generated one or more ultrasonic wave pulses to a sample container holding the biological tissue sample through a coupling medium that couples the one or more FASA elements to the sample container to dissociate single cells from the biological tissue is described herein.

Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.

Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.

A cell capture apparatus includes a cell separation unit having a flat plate shape and a cell capture unit having a flat plate shape located therebelow. A liquid sample and a carrier liquid flow in a cell-separation flow passage of the cell separation unit. The liquid sample contains multiple large cells, multiple small cells, and a sample liquid component. The cell-separation flow passage separates a set of large cells and the carrier liquid from a set of small cells and the sample liquid component. The cell capture unit includes a large-cell flow passage in which the set of large cells and the carrier liquid flows, and multiple electrode wires for attracting the large cells by means of dielectrophoresis. Multiple cell capturing wells are formed in the large-cell flow passage. Each of the multiple cell capturing wells has a size that can capture one of the large cells attracted by the electrode wires.

A device for manufacturing Antarctic chionodraco rastrospinosus skin antifreeze protein includes a de-frozen and cleaning device for de-frosting frozen grass carp skin and then cleaning carp skin; a skin slurry for making de-fat skin slurry; upper fat on the skin slurry being removed to get de-fat skin slurry; an ultrasonic generator for ultrasonic treatment to change organizational structure of skin antifreeze proteins of the slurry; a composite protease adder for adding composite protease added to the slurry; a collector receiving the slurry from the adder, and then the slurry are retained a time period and cooled to room temperature; then a 4000 g centrifugation is performed through 10 min; and a two-step filter connected to the collector for receiving the supernatant from the collector for performing a two-step ultra-filtration process so as to get skin antifreeze protein with molecular weight of 800010000 Dalton and.

A device for manufacturing Antarctic chionodraco rastrospinosus skin antifreeze protein includes a de-frozen and cleaning device for de-frosting frozen grass carp skin and then cleaning carp skin; a skin slurry for making de-fat skin slurry; upper fat on the skin slurry being removed to get de-fat skin slurry; an ultrasonic generator for ultrasonic treatment to change organizational structure of skin antifreeze proteins of the slurry; a composite protease adder for adding composite protease added to the slurry; a collector receiving the slurry from the adder, and then the slurry are retained a time period and cooled to room temperature; then a 4000 g centrifugation is performed through 10 min; and a two-step filter connected to the collector for receiving the supernatant from the collector for performing a two-step ultra-filtration process so as to get skin antifreeze protein with molecular weight of 800010000 Dalton and.

One or more methods for treating a tissue site comprises collecting a tissue sample that has keratinoytes and placing the tissue sample in a cartridge that has a well plate, positioning the cartridge within a base unit that has a tissue disaggregator, activating the base unit to rotate the well plate to align wells in the well plate with the tissue sample and the tissue disaggregator, operate the tissue disaggregator to separate the tissue sample to form a regenerative epidermal suspension, and provide the regenerative epidermal suspension to a tissue site to enhance healing of the tissue site.

Provided is a container for cell culture, provided with a container main body and injection/discharge ports, in which an inside of the container main body is divided into a plurality of cisterns 1st and 2nd by a filter member. Thus, various operations performed upon the cell culture can be simply and efficiently performed.

Provided is a method for extracting plasmid DNA in bacteria, realizing lysis and neutralization during plasmid production in two mixing assemblies connected in series, and comprising the following steps: (1) mixing, (2) lysing, and (3) neutralizing. Step (1) is completed in a first mixing assembly; step (2) is completed in a lysis helical tube; step (3) is completed in a second mixing assembly; and the first mixing assembly, the lysis helical tube and the second mixing assembly are sequentially connected in series. A device used in the plasmid preparation process is simple, is convenient to operate, is low in costs, can remove a large amount of impurities during cell lysis without professional customized device and expensive device, has safe components, realizes automatic continuous lysis, and facilitates industrial production.