Disclosed herein are systems and devices for wound therapy and related methods of use. The systems and devices for wound therapy and related methods of use disclosed herein may comprise one or more cartridges for preparation of a regenerative epidermal suspension. One or more cartridges for preparation of a regenerative epidermal suspension may comprise a cover with a raised processing opening, a cup that can be received within the raised processing opening and has a screen, and a well plate that is positioned under the cover, has a well, and rotates relative to the cover such that the well aligns with the raised processing opening. One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with an opening that can receive a tissue sample.

An apparatus and method using the apparatus for disruption of cell wall free cells without disrupting comprising biologically active compartments enclosed by a lipid bilayer. A shear force generating device generates a region of a shear force suitable for disrupting the cells. Optionally, a pump for pumping an aqueous medium including the cells with an adjustable flow rate or flow velocity, preferably adjusting the residence time and the shear force separately, and a separation device are combined for separating the at least one released biologically active compartment and/or its surrounding biologically active liquid phase from debris. The apparatus is a standalone system or an integral part of a production line. The apparatus and method enable the production of products including a high yield of biologically active compartments exhibiting a high biological activity, in particular in energy regeneration and/or protein synthesis.

One or more methods for automated preparation of a regenerative epidermal suspension comprises a processor receiving an initiation signal that indicates that a cartridge that has a cover, a cup containing a tissue sample, and a well plate situated beneath the cover that has a first well containing an enzyme solution and a second well containing a buffer solution, has been placed on a sensor, executing a sequence that actuates a tissue disaggregator against the tissue sample in the cup positioned in the first well, raises the cup to an upper position, rotates the well plate to position the second well beneath the cup, lowers the cup to a position within the second well, and actuates the tissue disaggregator against the tissue sample in the second well.

One or more methods for automated preparation of a regenerative epidermal suspension comprises a processor receiving an initiation signal that indicates that a cartridge that has a cover, a cup containing a tissue sample, and a well plate situated beneath the cover that has a first well containing an enzyme solution and a second well containing a buffer solution, has been placed on a sensor, executing a sequence that actuates a tissue disaggregator against the tissue sample in the cup positioned in the first well, raises the cup to an upper position, rotates the well plate to position the second well beneath the cup, lowers the cup to a position within the second well, and actuates the tissue disaggregator against the tissue sample in the second well.

Disclosed herein are systems and devices for wound therapy and related methods of use. The systems and devices for wound therapy and related methods of use disclosed herein may comprise one or more cartridges for preparation of a regenerative epidermal suspension. One or more cartridges for preparation of a regenerative epidermal suspension may comprise a cover with a raised processing opening, a cup that can be received within the raised processing opening and has a screen, and a well plate that is positioned under the cover, has a well, and rotates relative to the cover such that the well aligns with the raised processing opening. One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with an opening that can receive a tissue sample.

Disclosed herein are systems and devices for wound therapy and related methods of use. The systems and devices for wound therapy and related methods of use disclosed herein may comprise one or more cartridges for preparation of a regenerative epidermal suspension. One or more cartridges for preparation of a regenerative epidermal suspension may comprise a cover with a raised processing opening, a cup that can be received within the raised processing opening and has a screen, and a well plate that is positioned under the cover, has a well, and rotates relative to the cover such that the well aligns with the raised processing opening. One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with an opening that can receive a tissue sample.