A sterile bioreactor is formed of a combination of a bag and a bubble diffuser retained in the bag. The bubble diffuser is formed of a microporous sheet material that produces substantially uniform micro gas bubbles for enhancing plant organism growth within the bag. The bag is made in a cost-effective manner and with desired sterility by sealing coupling devices and/or ports, the tube ends sealed and gamma irradiated. The coupling devices/ports are established in one side wall of the bag. Contents may be inserted into and removed from the bag through the coupling devices/ports, which may be surface mounted portals.

Methods of enhancing biomass and secondary metabolite accumulation of microalgal species are described herein. A cell culture of the microalgal species were elicited using a combination of techniques for a period of time. Experimental studies compared biomass dry weight production, chlorophyll dry weight content and astaxanthin dry weight content between controls and elicitation treatments. The present invention demonstrated that combined elicitation is an effective method for improving cell biomass growth and astaxanthin production.

Provided is a continuous microalgae culture module, including an outdoor culture unit, a high-density culture unit, a pigment induced unit, and a harvesting unit. A method of culturing microalgae containing macular pigment is also provided, including sequentially culturing microalgae with medium in the outdoor culture unit and the high-density culture unit, producing macular pigment in the pigment induced unit through different light irradiation, and collecting the microalgal biomass containing macular pigment in the harvesting unit.

Devices, systems and methods for biological sensing and communication using optogenetics and electronics are described. One example method includes generating a light beam incident on multiple regions in a device, wherein each region comprises an optogenetic system to generate, upon interacting with the light beam, biosensors, wherein an interaction between the biosensors and stimulus molecules in each region is associated with a threshold for a production of an output molecule or an alteration of an output property of the output molecule, the biosensors, or the stimulus molecules, wherein the production or the alteration is based on a value associated with an information source, detecting an output received from one or more of the multiple regions corresponding to the output molecule or the output property in that region, and generating an electric signal associated therewith, and processing the electrical signal to determine the value associated with the information source.

A method for forming a product from a gas includes flowing a gas through a bioreactor for contacting the gas with polymer-immobilized whole cells and collecting the product from the bioreactor. The bioreactor includes a plurality of printed three-dimensional structures, each printed three-dimensional structure having at least one sidewall being a lattice, and the polymer-immobilized whole cells being present in the lattice.

The present invention provides for a method and transport system for conditioning micro algae grown in a commercial farm and transporting to a final destination while maintaining conditions that allow the microalgae to remain alive and healthy during transit.

The present disclosure is related to biosynthetic methods of forming monosaccharides, and systems for generating the same. A benefit of the methods and systems disclosed herein can include the sustainable production of monosaccharides in an automated process. A benefit of the methods and systems herein can be the generation of monosaccharides from renewable source materials. An additional benefit of the methods and systems herein can include the use of abundant feedstocks, such as carbon dioxide, for the efficient generation of select monosaccharides for use as nutrients and for other useful applications. Another benefit of the methods and systems disclosed herein can include reduction of excess carbon dioxide from the environment.

The present disclosure is related to electrochemical methods of forming monosaccharides, and systems for generating the same. A benefit of the methods and systems disclosed herein can include the sustainable production of monosaccharides in an automated process. A benefit of the methods and systems herein can be the generation of monosaccharides from renewable source materials. An additional benefit of the methods and systems herein can include the use of abundant feedstocks, such as carbon dioxide, for the efficient generation of select monosaccharides for use as nutrients and for other useful applications. Another benefit of the methods and systems disclosed herein can include reduction of excess carbon dioxide from the environment.

A technique for learning and steering evolutionary dynamics may include initializing a bioreactor including a population of evolving organisms; determining selection pressures; (a) applying the selection pressures to the population; (b) determining the population state and storing it in a population dataset; (c) detecting whether the population has reached a stable state; (d) if the population has reached the stable state: obtaining data representing the stable state, redetermining the selection pressures based on a selection pressure policy, and storing the data and the redetermined selection pressures in a stable state dataset; (e) determining whether one or more stopping criteria have been met; and repeating steps (a)-(e) until at least one of the stopping criteria is met.

An apparatus for enhancing bacterial sporulation can include a laminar flow chamber including multiple laminar flow tubes that smooth flow of coolant, a coolant input port that is coupled to the laminar flow chamber that supplies the coolant to the laminar flow chamber, a transparent tube section that is coupled to the laminar flow chamber and includes light-emitting diode (“LED”) light modules, the transparent tube section receives the smoothed flow of coolant from the laminar flow chamber, a culture solution tube that traverses through the laminar flow chamber and the transparent tube section, wherein a portion of the culture solution tube that is within the transparent tube section is surrounded by the coolant and is exposed to light from the LED light modules, and a culture solution input port that is coupled to the culture solution tube and supplies a culture solution to the culture solution tube.