The present application relates to a bioreactor system for culturing cells, especially cells without cell walls; the bioreactor system includes: a container containing a hollow cylinder with a diameter of D1 and a height of H1, and a hollow circular truncated cone with an upper diameter of D2, a lower diameter of D3, and a height of H2, where the hollow cylinder is connected to a top surface of the hollow circular truncated cone, and D1=D2; an oscillator, configured to cause the container to make an eccentric motion according to a certain eccentricity and rotational speed; a ventilation device, configured to introduce an oxygen-containing gas from an upper portion of the container to the inside of the container, and a culture solution filled in the container, of which a top surface is exposed to the oxygen-containing gas; where the oscillator is configured to maintain the eccentric motion of the container, such that a ratio of the total liquid surface area to the volume (S/V) of the culture solution when in a steady state of motion is 5.65 or more, the turbulence kinetic energy is 2.73E−03 m.sup.2/s.sup.2 or more, and the flow field shear rate is 20.27/s or less, where the total liquid surface area is the sum of the contact area between the culture solution and the reactor wall surface and the contact area between the top surface and the gas.

The present disclosure provides methods and materials for the cultivation and/or propagation of a photosynthetic organism. Such methods may comprise the use of a lamp assembly that comprises a plurality of circuit boards, each comprising at least three edges, arranged in a substantially spherical shape defining an interior lamp assembly volume, wherein the plurality of circuit boards comprise a first planar surface in contact with the interior lamp assembly volume and an opposing second planar surface comprising light emitting diodes (LEDs); and a barrier that surrounds the plurality of circuit boards forming the substantially spherical shape.

A photobioreactor is particularly suited for producing micro-organisms such as microalgae. The photobioreactor is a closed reactor with reactor vessels which have an open top that is closed by a top wall of the photobioreactor and in which a nutrient medium can be held. At least some of the reactor vessels are individual vessels. Adjacent reactor vessels form a gap between a front wall and a rear wall, the gap being closed at the top side by an overflow wall region and having a vessel overflow opening between the adjacent reactor vessels. A lighting element is held in the gap. Each of the reactor vessels has a partition which divides the reactor vessel into a front reactor chamber and a rear reactor chamber. At least one partition through-flow opening between the front and rear reactor chambers is formed in the partition close to the bottom wall.

A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.

A heating assembly for a bioreactor is disclosed. The heating assembly includes a holder having a plurality of segments coupled to each other to define a unitary structure and a heating component coupled to at least one segment of the plurality of segments. The unitary structure includes a top end, a bottom end, and a side wall. The side wall extends between the top end and the bottom end and along a circumferential direction of the heating assembly to define a cavity. At least a portion of the unitary structure has a gradually varied perimeter along a direction perpendicular to a plane which intersects the side wall and is parallel to the top end and the bottom end.

A variable diameter bioreactor vessel is provided that includes a first vessel section having a first diameter configured to hold a liquid media and biologic material, and a second vessel section having a second diameter that is greater than the first diameter such that the liquid media can be increased from a first volume to a second volume within the vessel.

The present invention is a composting bioreactor system that continually receives biodegradable solid wastes, waste waters and exhaust gases, automatically recycles the biodegradable wastes into nutrients and heat energy, and automatically supplies the nutrients and heat into an integrated hydroponic or aquaponic system. This invention together with integrated food growing system may be installed onsite such as balconies, backyards and premises of restaurants and food factories etc. therefore may lead to zero mileage targets both for recycling wastes and for supplying foods. This invention integrates composting process and aquaponic technology together and may establish a closed-loop recirculation of both water and gases therefore upgrades aquaponics into compoponics. A compoponic system has both soil and soilless growing beds and mimics nature recirculating nutrients, carbon and energy among human being, animals, microorganisms and plants by way of photosynthesis, slow burning by cellular respiration and burning by combustion.

The present invention relates to systems and methods involving interconnected plate components, including bases, lids and covers, interconnected in a manner such that a continuous strip of each component is prepared. The continuous strips may be stored as rollstock in a reel style. The physical properties of each continuous strip allow the base, lid and/or cover to include means for positive control. In one embodiment, the continuous strip of bases is advanced and processed through an automated system with positive control, and remains in the form of a continuous strip until agar in the bases is cured, at which time the bases are singulated. When a lid is applied to a base using methods described herein, an airtight seal is formed improving the quality of culture media used in testing.

A variable diameter bioreactor vessel is provided that includes a first vessel section having a first diameter configured to hold a liquid media and biologic material, and a second vessel section having a second diameter that is greater than the first diameter such that the liquid media can be increased from a first volume to a second volume within the vessel.

To provide a closed system culture vessel for realizing culturing in which an aseptic state is maintained at the time of culturing, and making the reduction of the shear stress generated during conveyance after manufacturing possible. A closed system culture vessel 201 holds a second vessel 302 consisting of an insert vessel on the inside of a first vessel 301, and a lid portion 303 of the closed system culture vessel 201 forms a closed space with the first vessel 301 and has a convex portion 305 contacting the entire outer circumference of the second vessel 302. During conveyance, the closed system culture vessel is conveyed in a state in which the culture medium for conveyance is filled within the second vessel 302.