A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.

A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

A bioreactor system includes a vessel having a bottom floor, a flexible bioprocessing bag disposed within the vessel, and a flexible bladder positioned intermediate the bottom floor of the vessel and the bioprocessing bag. The flexible bladder is selectively inflatable to vary at least one of a geometry or configuration of the bioprocessing bag to provide for improved drainability or an increased turndown ratio for the bioreactor system.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

The present invention relates to the design, fabrication and applications of three-dimensional (3D) bioreactor for cell expansion and cell secreted substance production. The bioreactors have relatively low levels of potentially cytotoxic compounds, can be coated with substituted or unsubstituted poly(p-xylene) type coatings and can also be separately formed from liquid crystal photopolymerizable monomers.

A culture device and a culture method by which a substance to be cultured can be three-dimensionally cultured, and the substance thus cultured can be removed as an integrated product with multiple tubes; and a cultured organ produced by the culture method. The culture device comprises a sealed container (2) which contains the substance to be cultured (A) and is disassembled after the culture is completed; multiple ducts (3, 4, 5) arranged in the sealed container (2) and have micropore formed on the outer peripheral surface; a culture medium-feeding device (6, 7) for feeding/circulating the culture medium (B) to at least one duct (3 or 5); an excretory device (8) connected to at least one duct (4) for excreting the waste product (C) permeating into the duct (4) through the micropore of the duct (4) from the substance to be cultured to the outside of the sealed container.

A bioreactor capable of producing complex, three-dimensional tissue constructs has improved media transfer and increased controllability with regard to exposure to the external environment. The bioreactor includes an external surface, a first tissue culture side for culturing a first cell source in a first tissue culture support region, a second tissue culture side for culturing a second cell source in a second tissue culture support region, and a plurality of ports. At least one of the ports of the plurality of ports extend from the first tissue culture support region of the first tissue culture side to the external surface. The plurality of ports can include an external port that is configured to be a liquid inlet when the bioreactor is in a first orientation and a gas outlet when the bioreactor is in a second orientation.

A microplate for containing a plurality of samples includes a plurality of sample cavities, each sample cavity including at least one dividing element which divides the sample cavity into at least a donor compartment and a receiver compartment. The dividing element is configured to contain one of the samples within one of the donor or receiver compartments when the microplate is in a first orientation. The dividing element is further configured to allow the sample to be transferred at least from the donor compartment to the receiver compartment of one of the sample cavities when the microplate is in a second orientation. A method for transferring a sample between compartments of the microplate is also disclosed.

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.