The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135−45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g.

Furthermore, the invention relates to a method for modifying cells comprising the steps introducing cells in a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135−45° to the rotational axis of the centrifugation chamber wherein and comprising at least one input/output port, immobilizing the cells on the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g maintaining the rotation of the rotation of the centrifugation chamber until the cells are modified.

Nano- to microscale liquid coacervate particles are provided. The liquid coacervate particles are produced by a process including stimulating a population of liquid droplets containing one or a mixture of components to induce a phase separation point of a first component, and maintaining stimulation at the phase separation point to form a coacervate domain of the first component within each of the droplets to form the liquid coacervate particles. The self-assembled nano, meso, micro and macro liquid coacervate particles and related coated substrates can have utility in drug delivery, bioanalytical systems, controlled cell culture, tissue engineering, biomanufacturing and drug discovery.

The tissue regeneration platform of one embodiment may exhibit excellent biocompatibility and tissue culture property on various supports, comprising a support and a coating layer disposed on the support, and comprising a hyaluronic acid-catechol compound.

Polymer hydrogels and methods for selective retrieval of microbial targets from microwells and other cell culture devices. The methods use semi-permeable, photodegradable hydrogel membranes that permit exchange of nutrients and waste products but seals motile bacteria and other microbes within microwells. Light exposure can be used to degrade the hydrogel membrane in a targeted manner and release the microbes from targeted microwells for further study.

The present invention relates to photobioreactors and more particularly to the use of a transparent composition based on at least one methacrylic polymer for constructing installations for the culture of photosensitive organisms. This composition can be in the form of films, plates, profiled elements or cylinders such as tubes. The invention also relates to a transparent multilayer structure comprising at least one layer of a methacrylic polymer and one layer comprising at least one antifouling additive, and to the use thereof for constructing installations for the culture of photosensitive organisms.

A cell aggregate culture vessel (1) is a vessel for culturing a cell aggregate. The cell aggregate culture vessel (1) includes a well (21) having a culture space capable of storing the cell aggregate and culture fluid and a tubular body (3) which is disposed on the well on a plane at which the well has an opening and have an inner cavity communicating with the culture space, and one or more communication portion (3a) capable of discharging the culture fluid without allowing passage of the cell aggregate to the outside of the tubular body (3) is formed in a tubular wall of the tubular body (3). The communication portion (3a) may be, for example, a slit having a width of 0.1 mm or larger and 0.5 mm or smaller.

This disclosure provides a cell culture media extending material capable of releasing nutrients into the cell culture environment slowly over time. In embodiments, this material is a part of a cell culture vessel. In embodiments, the material is a coating or a film on a surface of a cell culture material. In additional embodiments, the material is a surface upon which cells are cultured, such as a cell culture vessel or a microcarrier.

A cell culture vessel including an accommodating part which is an inner space accommodating a cell culture support therein, a fixing member configured to fix the cell culture support to a lower surface of the accommodating part, wherein the fixing member includes a first adhesive layer attached to the lower surface of the accommodating part, a second adhesive layer attached to a lower surface of the cell culture support, and a support film interposed between the first adhesive layer and the second adhesive layer and configured to perform a support function, and an adhesive force between the second adhesive layer and the cell culture support is higher than an adhesive force between the first adhesive layer and the lower surface of the accommodating part.

An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

The invention relates to a biocompatible scaffold for three dimensional cultivation of cells, said scaffold comprise one or more fibers randomly oriented to form a scaffold with open spaces for cultured cells. The one or more fibers are also coated with a bio-active coating and have a diameter of 100-3000 nm.