A culture plate including: an array of light sources formed inside and on top of a semiconductor substrate; a transparent planarization layer coating the array of light sources; and an array of wells formed on said layer, each light source being located vertically in line with a well.

A method for directly bonding a metal to a transparent substrate includes providing a substrate; placing a metal foil directly on a face of the substrate; irradiating a portion of the metal foil with a laser beam so that metal corresponding to the portion melts and bonds directly to the substrate and forms a metal pad; and pumping a gas above the portion to prevent oxidation of the melted metal.

In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.

A method for manufacturing a skin chip according to an exemplary embodiment of the present disclosure may include: a step of forming first and second PDMS layers disposed on both surfaces of a porous membrane and each having a microfluidic channel through which a culture medium is transferred to both surfaces of the porous membrane; a step of forming first and second MEA substrate layers disposed on the outer surfaces of the first and second PDMS layers, respectively, and having metal electrodes for measurement of TEER arranged at positions corresponding to the channels; and a step of forming first and second PMMA layers disposed on the outer surfaces of the first and second MEA substrate layers, respectively. In the method for manufacturing a skin chip according to in an exemplary embodiment of the present disclosure, the porous membrane may be made of a polycarbonate having pores of a predetermined size.

The present invention relates to poly(alkylene terephthalate) polyesters having long poly-methylene segments and their use in a wide variety of applications. Particularly, said PAT polyesters are used in biotechnological or biomedical applications, wherein the extent of cell adhesion, cell growth or cell interaction, in particular endothelial cells, depends on the odd or even number of carbon atoms in the aliphatic segments. Also provided are methods for the preparation of poly(alkylene terephthalates) (PAT) having long poly-methylene segments, wherein the bifunctional monomers, in particular terephthalic acid (or a derivative thereof) and an aliphatic diol, are dissolved in a solvent and the polycondensation reaction takes place in solution.

A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing an effective wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.

The present invention provides methods for optically micropatterning hydrogels, which may be used for, e.g., regenerative medicine, synthetic or cultured foods, and in devices suitable for use in high throughput drug screening assays.

Systems and methods are provided including a housing configured to receive and engage a hollow tissue structure within a fluid chamber of the housing. A first pair of flow channels and a second pair of flow channels of the housing are fluidly coupled to the fluid chamber. The housing fluidly couples the first pair of flow channels and fluidly couples the second pair of flow channels via a second flow path such that a change in a fluid pressure differential between a first fluid in the first flow path and a second fluid in the second flow path deflects at least a portion of the hollow tissue structure causing a change in flow of the first fluid through the first pair of flow channels or a change in flow of the second fluid through the second pair of flow channels.

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.