A method for separating cells may include providing a sample having at least two different types of cell, contacting the sample with a cell culture substrate comprising a stimulus-responsive polymer layer, subjecting the cell culture substrate and the cells to medium conditions to where the cells adhere to the cell culture substrate, and modifying the medium conditions to decrease the adherence of one of the cells types to the cell culture substrate. The method may further include separating the cells released from the cell culture substrate from the cells still adhered to the cell culture substrate.

A cell culture bag includes a body formed of a composite film including a first fluoropolymer and a second fluoropolymer. The body defines a cell culture compartment configured to hold a cell culture. The first fluoropolymer has a first thickness and the second fluoropolymer at least partially impregnates the first thickness of the first fluoropolymer. The composite film has a second thickness from 0.01 mm to 0.059 mm and a tensile strength in one direction of 10,000 psi to 92,000 psi. The composite film also has O.sub.2 flux of 2,000 cm.sup.3/M.sup.2/atm/day to 20,000 cm.sup.3/M.sup.2/atm/day and a total transmittance from 70% to 100%. A cell culture assembly includes a plurality of the cell culture bags connected in series. A cell culture container includes a cell culture compartment and a composite film connected to the cell culture compartment, the composite film including a first fluoropolymer and a second fluoropolymer.

The invention relates to a cell culture bottle for adherent cells (e.g. human mesenchymal stem cells), comprising: a vessel; an internal cylinder, which has an internal Archimedes screw; an internal central tube, through which liquid can flow; and at least one wall arranged around the central tube. This arrangement provides an enlarged inner surface for the growth of the cells and for the reliable mixing of the fluid. The cell culture bottle is formed as a single piece and can be simply and economically produced as a disposable device by means of additive manufacturing.

A scaffold material for cell culture having excellent cell colonization is provided. A scaffold material for cell culture according to the present invention includes a synthetic resin having a main chain and a graft chain, wherein the graft chain has a polydentate hydrogen-bonding group represented by the following formula (1) in a side chain: *—R-(A.sub.i).sub.n (1) wherein R represents a group having 50 or less carbon atoms, A.sub.i represents an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, i represents a natural number, n represents an integer of two or more and four or less, and * represents a bonding position with another atom constituting the graft chain; and A.sub.i may be identical or different from each other.

This is to provide a coating film having a function of inhibiting adhesion of a biological substance, a method for manufacturing the coating film, a copolymer obtainable by polymerizing a specific monomer mixture, a composition for forming a coating film having a specific composition, a method for manufacturing a varnish containing a copolymer to be used as a raw material of the composition for forming a coating film which is used for forming said film, and a sol for forming the coating film. In particular, this is to provide a coating film obtained by the method comprising a process of applying a composition for forming a coating film which comprises a copolymer comprising a recurring unit containing an organic group of the formula (a) and a recurring unit containing an organic group of the formula (b) and a solvent onto a substrate; and a process of drying at a temperature of −200° C. to 200° C. (wherein U.sup.a1, U.sup.a2, U.sup.b1, U.sup.b2 and U.sup.b3, and An.sup.− are as defined in the present specification and the claims). ##STR00001##

The present disclosure relates to a nanofiber structure for cell culture, a method for manufacturing the structure, and a cell analysis device including the nanofiber structure for cell culture. The structure includes a cell culture layer made of nanofibers; and a spacer protruding upward from a surface of the cell culture layer, wherein the spacer divides a region on the cell culture layer into at least two culturing regions, wherein the spacer is made of the same nanofibers as the cell culture layer and thus has a cell migration channel defined therein.

A cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. In an embodiment, the device is used as a point-of-care and/or portable device. Further, the present disclosure describes software that, when executed by a processor, causes the device to perform the disclosed functions.

A cell culture cartridge is provided comprising a plurality of zones geometrically configured to provide for symmetrical fluid flow with each of the plurality of zones to avoid dead areas in flow within each of the plurality of zones. In certain embodiments, at least eight inlets are provided, with an inlet positioned at each corner of the cell culture cartridge. In certain embodiments, a shared outlet is positioned on a top surface of the cell culture cartridge.

Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.

Provided are a method for producing undifferentiated cell spheroids, the method comprising the step of culturing undifferentiated cells on a cell-adhesive surface of a cell culture sheet; a method for maintaining cell spheroids in an undifferentiated state, the method comprising the step of culturing undifferentiated cell spheroids on a cell-adhesive surface of a cell culture sheet; and a cell spheroid obtained by the method.