CO.sub.2, and other gases are utilized with mineral feedstock to synthesize products. The synthesized products, as the result of liquid, solid, gas photo-chemical reactions within the advanced bioreactor of the disclosed embodiment, are precipitated raw material for multiple consumer and industrial products. Waste heat, pressure and torque produced from the bioreactor are utilized for generating electricity and/or heat through a combination of energy recovery devices. Energy recovery devices offsets and lower the cost of operating the reactor as the disclosed reactor integrates photolysis via ultra-violet light, as an integral component, of a reactor system, composed also of an active mixer-agitator assembly, pressure and vacuum vessel chamber, heat source, and ports for media ingestion. The disclosed reactor is designed be conducive to transforming gaseous, solid, and liquid feedstock, like carbon dioxide —CO.sub.2, and other feedstocks that are inorganic and/or organic in an aqueous medium, into inorganic and organic products.

A resealable microbial culture and observation device provides a growth platform for the growth, transportation, and inspection of a biologic organism. The device provides a tray having at least one growth medium depression for containing a growth medium and a biologic, at least one anerobic member depression for containing an anerobic member that creates an anerobic environment for the biologic, and a vent to enable filtered gas exchange with the ambient environment, so as to supplement the microbial growth conditions in the growth medium depression during single use or in stacked position of multiple devices; and further having a resealable label with a nontransparent portion for labeling, and a transparent portion for viewing the biologic.

Through sourcing net-primary productivity additive algae-based biomass feedstock, the exclusive use of renewable energy in processing, and the appropriate formulation and processing, a novel algae-derived bio-based plastic is both carbon-negative and provides some performance advantages over existing algae-based film plastics especially with regard to optical clarity. A system may be provided that produces a carbon-negative bioplastic. The production of the bioplastic in a process chamber may be controlled by an electronic controller. The electronic controller may be controlled by a host system, such a server. The electronic controller may be configured to direct production of the bioplastic in the process chamber using hydrocolloid, which is derived from algae.

Multiplexable microfluidic culture chamber for imaging monolayer growth of single cells The present invention relates generally to a microfluidic device (1a, 1b), particularly for use in single cell analysis. More specifically, the present invention relates to a microfluidic device (1a, 1b) comprising at least one chamber (10), in particular at least two chambers (10) comprising a deformable membrane (16) and having a structure and geometry configured to enable formation of two-dimensional cell culture, in particular two-dimensional cell growth area (29), and imaging thereof over a growth period or a time period sufficient to analyze cells, in particular to monitor cell growth. The microfluidic device (1a, 1b) allows for multi-condition operation of single-cell screening at high spatiotemporal resolution. The present invention also relates to methods for fabrication and use of such devices.

A microplate assembly for a plurality of samples includes a donor microplate having a plurality of sample donor cavities. The microplate assembly further includes a receiver microplate having a plurality of sample receiver cavities each sample receiver cavity having a transparent receiver bottom configured to enable microscopic imaging. In addition, the microplate assembly includes a leak-tight connecting structure configured to assemble the donor microplate and the receiver microplate, with at least one of the sample donor cavities being in communication with at least one of the sample receiver cavities. Further aspects are a receiver microplate and a leak-tight connecting structure for a microplate assembly as well as a method for transferring samples by means of a microplate assembly.

A cell culture chip comprises a body having a first flow channel and a second flow channel at least part of which overlaps the first flow channel when seen from an angle parallel with a predetermined direction, a cell separation membrane having first and second principal surfaces facing away from each other, the cell separation membrane being disposed between the first flow channel and the second flow channel so that the first flow channel is located on the first principal surface and the second flow channel is located on the second principal surface, a first electrode which is in contact with the first flow channel and extending through the first flow channel along the first flow channel, and a second electrode which is in contact with the second flow channel and extending through the second flow channel along the second flow channel.

An exemplary embodiment of the present disclosure provides A method and system for forming a microscale cell-laden matrix using an aqueous two-phase system (“ATPS”) comprising a mixture of a first material and a second material having a phase boundary between the first and second materials. The method can comprise mixing an enzyme with the first material, mixing a protein with the second material, and mixing a suspension comprising cells with one of the first material or the second material, wherein the enzyme, protein, and suspension comprising cells generate the cell-laden matrix and wherein the first material comprises a first polymer comprising polyethylene glycol and the second material can be a second polymer selected from the group consisting of dextran, polyvinyl pyrrolidone, polyvinyl alcohol, or ficoll.

The invention relates to a process and equipment for cultivating and producing a biomass from microalgae, primarily plankton. A microalgal biomass is cultivated in a single bioreactor chamber in the shape of a vertically oriented parallelepiped. The culture mixture is illuminated by artificial light sources mounted on the inner side of one of the broad walls of the bioreactor chamber in horizontal rows throughout the height of the bioreactor chamber. The culture mixture is illuminated cyclically. Spray heads are mounted on the inner side of the opposite wall of the bioreactor chamber so that 4 spray heads are arranged symmetrically around each artificial light source. The pH value of the culture mixture is maintained in a range of 8.5-9.5 by the addition thereto of a lactic acid bacteria-containing solution at the beginning of each light cycle in an amount of 1-3 ml per litre of culture mixture, said solution having a pH value selected in a range of 4.0-5.0.

A cell culture bag includes a body formed of a composite film including a first fluoropolymer and a second fluoropolymer. The body defines a cell culture compartment configured to hold a cell culture. The first fluoropolymer has a first thickness and the second fluoropolymer at least partially impregnates the first thickness of the first fluoropolymer. The composite film has a second thickness from 0.01 mm to 0.059 mm and a tensile strength in one direction of 10,000 psi to 92,000 psi. The composite film also has O.sub.2 flux of 2,000 cm.sup.3/M.sup.2/atm/day to 20,000 cm.sup.3/M.sup.2/atm/day and a total transmittance from 70% to 100%. A cell culture assembly includes a plurality of the cell culture bags connected in series. A cell culture container includes a cell culture compartment and a composite film connected to the cell culture compartment, the composite film including a first fluoropolymer and a second fluoropolymer.

Disclosed is a process for growing adherent cells in a containment box of a multi-parallel bioreactor, including: seeding the adherent cells on a carrier held in a culture dish; transferring the adherent cells on the carrier to a containment box of the multi-parallel bioreactor; and growing the adherent cells at a containment box while agitating the media at an impeller speed between 200 rpm to a 1200 rpm.