2D and 3D cell culture containers formed from blends of poly-L-lactide and poly-D-lactide provide growth surfaces for adherent cells and do not require surface treatment or coating to support mammalian cell growth. The cell culture containers are transparent, heat tolerant, and are environmentally degradable and suitable for composting in landfills.

The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.

Microfluidic chips, systems, and methods of making and using thereof are described. The microfluid chip includes a disc-shaped transparent layer containing a plurality of cell traps, pillars, and filers, and a support layer attached thereto. The microfluid chip has at least one inlet port in center of the transparent layer for receiving a sample of liquid and cells, and optionally a plastic cover. The microfluid chip can be designed to be suitable for the forces used for cell pairing/fusion in stationary and spinning format, or suitable for a particular cell fusion method such chemical and electrical methods. The microfluid chip is particularly suited for fusing dendritic cells and tumor cells for immunotherapy, or for generating hybridoma.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

A bioreactor system for receiving a disposable bioreactor bag comprises a receiving container having a container wall which defines a receiving space in which the disposable bioreactor bag is received in an operating state of the bioreactor system. A stirring system projects at least partially into the receiving space and is designed and configured to stir a biomedium present in the disposable bioreactor bag in the operating state of the bioreactor system. At least one baffle, which makes the receiving space smaller and differs from the container wall, serves to reduce a laminar flow of the biomedium. A temperature control medium flows through at least part of the at least one baffle, said temperature control medium controlling the temperature of the baffle.

A cell culture system or assembly, flask, plate and dish for growing, viewing and evaluating cells; and methods of use.

Systems and methods for culturing and/or analyzing cells are provided. The system can include a microfluidic layer, a multi-well grid layer and a base layer. An electrode layer can optionally be provided. The system can also include an alignment feature for aligning microchannels of the microfluidic layer with electrodes of the electrode layer to achieve a predetermined organized architecture of the microchannels relative to the electrodes. The system can include a plurality of microfluidic layers and a microfluidic layer engaging frame engageable with the plurality of microfluidic layers to form a unitary structure engageable with a multi-well plate comprising a plurality of wells each comprising an electrode layer. A well identification feature associated with a microfluidic unit can be provided on an upper surface of the multi-grid layer to enable visual identification of a well of the multi-well grid layer that is in fluid communication with a microfluidic unit.

A cell culture device comprising a fixed bed arranged in a housing, the fixed bed comprising a continuous pleated porous medium comprising a top end, a bottom end, a front side, a rear side, a right side, and a left side, and a plurality of pleats folded on the right side and the left side, and having vertical fluid flow channels along the longitudinal axis between adjacent pleats, is disclosed.

In some variations, a UV-sterilizable bioreactor system comprises: a chamber configured to carry out a reaction; a component configured for introducing a gas into the chamber; and one or more UV light sources configured to expose ultraviolet light to surfaces within the chamber. Some variations provide a method of cleaning and sterilizing a bioreactor, comprising: providing a bioreactor system with a chamber to carry out a reaction, a component for introducing a gas into the chamber, and one or more UV light sources to expose ultraviolet light to chamber surfaces; cleaning the chamber; and exposing the chamber to UV radiation to sterilize the chamber. The disclosed technology is a fundamental advance in the field of sparged bioreactors, for many types of commercial fermentations. The bioreactor may incorporate materials that are expressly incompatible with steam sterilization, which allows for the use of lower-cost materials, among many other benefits from UV sterilization.

The present invention relates assembly and a system for improved, high-throughput cost-effective cell culture, cultivations, fermentations and assays. Taking advantage of an assembly which can adopt two different configurations, efficient methods for monitoring a plurality of reaction systems and/or for cultivating and/or screening cell populations are provided.