Disclosed is a cell collection device with which switching and seeing microscope images with different magnifications can be attained in one-touch without rotating a revolver. The cell collection device includes an optical system to observe cells contained in a transparent vessel under magnification. The optical system includes a first optical system to observe the cells from upper side and a second optical system to observe the cells from lower side. The first optical system and the second optical system have different magnifications, and the optical axis of the first optical system and the optical axis of the second optical system are coincident.

A perfusion plate that can be combined with pillar plates containing cell layers is disclosed. The perfusion plate can have an inflow reservoir and an outflow reservoir connected by at least one channel, which fluidly connects the perfusion wells to the reservoirs for the flow of a fluid such as growth media. A perfusion plate can be part of an assembly containing a pillar plate, a lid, and a transparent bottom for visualizing cell growth in the perfusion wells. The perfusion-pillar plate assembly can facilitate perfusion-based tissue culture and tissue communication for high throughput, high-content, drug screening.

A method of forming a high-throughput, three-dimensional (3D) microelectrode array for in vitro electrophysiological applications includes 3D printing a well plate having a top face and bottom face. A plurality of culture well each includes a plurality of 3D printed, vertical microchannels and microtroughs communicating with the microchannels. The microtroughs and the microchannels are filled with a conductive paste to form self-isolated microelectrodes in each of the culture wells and conductive traces that communicate with the self-isolated microelectrodes.

Methods, systems, and devices for non-invasive measurement of cell cultures are described that provide for remote monitoring of cell status. The system includes a cell culture vessel, at least one monitoring layer, at least one measurement device, and a communication component. The cell culture vessel may include at least one cell culture chamber configured for cell growth and for closed-system operation. The monitoring layer is external to the at least one cell culture chamber. In some cases, the communication component is configured to transmit data from the monitoring layer to a remote location.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

A composition, including a substrate having a planar array of depressions each defined by concave walls and a moat disposed around each depression of said array of depressions.

The present invention provides a cell culture vessel/sample observation cell that is capable of culturing cells or cell tissue in three dimensions and allowing a three-dimensional structure of the cells or the cell tissue to be perceived easily. The cell culture vessel/sample observation cell according to the present invention for containing a culture gel in which the cells or the cell tissue is embedded, comprises a frame, at least one window bordered by the frame, and at least one projection projecting from the frame inwardly into the cell culture vessel/sample observation cell, wherein the at least one window is light-permeable and nutrient component-permeable and is placed in such a way as to allow for multifaceted observation of the cells or the cell tissue, and the projection has a feature point to be placed at a position where the cells or the cell tissue as well as the feature point can be observed from the window.

The present disclosure describes a manufacturing method to use algae as a renewable green factory for producing biodegradable bioplastic. One or more embodiments include separation of a cultivated microalgae biomass from water before use in the wet or dried state. The lipids and proteins are extracted from the biomass which leaves starch and algae precursors in the remaining material from the microalgae cells. The starch includes amylose, amylopectin, monosaccharides kinases and cyclobutadiene and is hydrolyzed into a syrup containing oligosaccharides and polysaccharides. In some cases, the syrup is used as an ingredient in a medium containing nutrient for bacterial fermentation of plastics.

An insect culture maintenance system includes a sequence of open-ended cylindrical tubes [500, 502, 504, 506] joined pairwise alternately at their tops and bottoms using multiple dual-cap connectors. Each dual-capped connector has a channel from an inside of a first cap to an inside of a second cap. In use, connectors that cap the bottoms of the tubes [508, 512] are filled with insect food media [518, 520], while connectors that cap the tops of the tubes [510] are open. As a result of this design, adults pass from one tube to the next through the top dual-cap connectors, while larvae pass from one tube to the next through the bottom dual-cap connectors, resulting in propagation of subsequent generations of insects through the sequence of tubes.

A bioreactor includes a culture medium vessel housing culture medium. The culture medium vessel further includes a first side surface including a first transparent optical window; and a second side surface parallel to the first surface and including one or more sensor adapters to fix optical sensors. A cell retention vessel is disposed underneath and connected to the culture medium vessel, the cell retention vessel housing biological cells and having: a top surface that intersects a base of the culture medium vessel, and a second transparent optical window indented into the top surface at a first corner of the top surface. A semipermeable membrane is disposed at a bottom of the cell retention vessel, and a frame comprising a grid is disposed underneath the semipermeable membrane.