Control of humidity in chemical reactors, and associated systems and methods, are generally described. In certain embodiments, the humidity within gas transport conduits and chambers can be controlled to inhibit unwanted condensation within gas transport pathways. By inhibiting condensation within gas transport pathways, clogging of such pathways can be limited (or eliminated) such that transport of gas can be more easily and controllably achieved. In addition, strategies for purging condensed liquid from chemical reactor systems are also described.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

A cell culture device includes a control unit that includes a first gas supply source, a second gas supply source, and a decompression source, and a culture unit that includes a first container, a second container, a first gas channel that connects a third container, the first gas supply source, the second container to a connection point, a second gas channel, a third gas channel, a first liquid channel, a second liquid channel, a gas-liquid channel, a first valve, a second valve, a third valve, a fourth valve, and a fifth valve.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

The present disclosure relates to bioreactors and bioreactor facilities that include a plurality of bioreactor tiles. One example system includes a system that includes a first bioreactor. The first bioreactor include a first housing. The first bioreactor also includes a plurality of first bioreactor tiles positioned within the first housing. Additionally, the first bioreactor includes a first fluid management system. Further, the first bioreactor includes a first illumination system. In addition, the first bioreactor includes a first environmental control system. Still further, the first bioreactor includes one or more first sensors positioned within the first housing. Yet further, the first bioreactor includes a first controller configured to receive data from the one or more first sensors and provided, based on the received data, one or more operating parameters to the first fluid management system, the first illumination system, or the first environmental control system.

Described is an apparatus for culturing cells or tissue, the apparatus comprising a container comprising a bottom and at least one sidewall, wherein at least a part of the bottom comprises a gas permeable material or is adapted to engage with a gas permeable material and is perforated to allow gaseous exchange; a detachable top adapted to engage with the container to define a chamber, wherein at least a part of the top comprises a gas permeable material or is adapted to engage with a gas permeable material and is perforated to allow gaseous exchange; and a scaffold adapted to receive a substrate for cells to reside upon. The apparatus is configurable between (a) a first mode in which the substrate is not disposed in gaseous communication with a gas permeable material, and (b) a second mode in which the substrate is disposed in gaseous communication with a gas permeable material.

An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm.sup.2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm.sup.2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×10.sup.6 cells/cm.sup.2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×10.sup.5 cells/cm.sup.2.