A device for culturing cells in a shaking operation with passive gas exchange, which includes at least one gas exchange opening, characterized in that the opening surface of the gas exchange opening is not oriented parallel with the movement vector of the shaking movement at at least one point in time of the shaking movement. Owing to the shaking movement and the ambient gas phase flowing around the outside of the device and the headspace gas phase flowing around the inside of the device, which flowing is associated with the shaking, the arrangement leads to the formation of a pressure gradient between the headspace gas phase and the ambient gas phase, which pressure gradient changes depending on the shaking movement and advantageously influences the gas exchange between the headspace gas phase and the ambient gas phase and the gas blending within the headspace.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.

Provided are cell culture apparatus and method. The cell culture apparatus comprises a culture column and a perfusion system, the culture column comprising a first and a second tube-connecting caps and a support for cells to attach and grow thereon. The first and the second tube-connecting caps are respectively provided with a perfusion inlet and a perfusion outlet; The perfusion system comprises a culture medium storage device, a perfusion tube and a perfusion pump. A complete and closed culture column is formed in the cell culture device after the cells are inoculated, and the culturing is completed by the perfusion system to avoid contamination.

A system for irradiating biological material includes a platform for holding the biological material and a radiation source for directing X-ray radiation to the biological material. The platform includes platform elements each including a chamber for containing the biological material. The platform includes a frame structure for mechanically supporting the platform elements and for mechanically supporting a gas-supply system for supplying gas to the platform elements. The gas-supply system can be for example a gas cartridge. The frame structure includes gas channels for receiving the gas from the gas-supply system and for conducting the gas to the platform elements so as to provide a desired gas composition in the chambers during irradiation of the biological material.

A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

A cell culture device includes a control unit that includes a first gas supply source, a second gas supply source, and a decompression source, and a culture unit that includes a first container, a second container, a first gas channel that connects a third container, the first gas supply source, the second container to a connection point, a second gas channel, a third gas channel, a first liquid channel, a second liquid channel, a gas-liquid channel, a first valve, a second valve, a third valve, a fourth valve, and a fifth valve.