A packaging for a bioprocessing bag includes a housing having an open interior space, and a support base attached to an external side of the housing, the support base having a recess for receiving an impeller base plate of a bioprocessing bag.

Aspects of the invention relate to cell culture vessels and method for using the vessels to agitate and maintain cells in suspension. In some embodiments, the vessel has a body configured to hold a volume of liquid containing cells to be cultured, the vessel body having a deformable portion arranged to induce movement of the liquid sufficient to maintain the cells in suspension. In some embodiments, the deformable portion is in at least one of a base and sidewall of the vessel body. In some embodiments, the deformable portion is deformed according to a deformable pattern. In some embodiments, the deformable portion is deformed via a deformation plate having one or more movable members that contact the deformable portion.

The present set of embodiments relate to a system, method, and apparatus controlling a cell culture media mixing system. The control system includes an integrated control unit capable of controlling a broad ranges of peripheral devices commonly used in bioproduction through a graphical user interface displayed on a touch sensitive screen. The bioproduction system is designed to be highly customizable through process modification and recipe creation by user with little or no knowledge of programming and is capable of controlling a wide variety of devices using a single unit. The bioproduction system allows for auto-detection, auto-calibration, and automatic of device related processes into a bioproduction workflow.

A bioreactor chamber (1) including a first end block (4), a second end block (6) and a flexible membrane (2). The flexible membrane (2) extends between the first end block (4) and the second end block (6) and defines a cavity (10) bounded by at least the flexible membrane (2). The cavity (10) is arranged to receive a substrate, for growing a culture on the substrate or a biomaterial for testing the biomaterial.

The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

A biological component cassette includes a cassette main body having a flow path for the liquid in an interior of the cassette main body. The cassette main body is flexible and has a sheet-like shape. The biological component cassette includes a frame that is less flexible than the cassette main body. The frame includes an accommodation chamber in which the cassette main body is accommodated. A side portion of the frame forms at least part of the accommodation chamber. When the cassette main body is accommodated in the accommodation chamber, one surface of the cassette main body is covered by a bottom portion of the accommodation chamber, and another surface of the cassette main body is exposed to an external environment.

An in vitro endothelial cell culture system for optimizing the pulsatile working mode of a continuous flow artificial heart belongs to the technical field of artificial organs. The system includes three parts: 1) a cell culture model on a microfluidic chip and an off-chip multielement aortic arch afterload fluid mechanics circulation loop; 2) devices for simulating the power source of a cardiovascular system: a fluid loading device is realized by a pulse blood pump, and an artificial heart device is connected in parallel to both ends of the pulse blood pump; and 3) a peripheral detection and feedback control system, comprising pressure and flow sensors, a fluorescence microscope, a CCD high-speed camera system and a proportional-integral-derivative feedback control system. The system can accurately simulate the real hemodynamics microenvironment of vascular endothelial cells in different parts of the aortic arch.

A reactor system includes a support housing having an interior surface bounding a chamber, the chamber having a vertically extending central longitudinal axis. A flexible bag is disposed within the chamber of the support housing and has an interior surface bounding a compartment. A mixing element is disposed within the compartment of the flexible bag. A drive element, such as a drive shaft, is secured the mixing element, wherein the mixing element is laterally offset from and/or is angled relative to the vertically extending central longitudinal axis of the support housing.

A bioreactor for producing hydrogen gas and other metabolites. The bioreactor utilizes light, fermentation, and other metabolic processes for the production of metabolites, derived from various microorganisms contained within the bioreactor through respective metabolic pathways. The bioreactor comprises a main reactor chamber, a semipermeable membrane, a sleeve, a power supply, a substrate medium, a heating member, a plurality of tubing members, a collection reservoir, a pressure-sealed connecter member, and an agitator.

The invented bio-electrical system is a housing-electrode which allows insertion of another electrode for various electrochemical and bio-electrical applications. Together with other invented elements as well as standard components, the system is fully scalable, modular, and allows production and collection of gases under pressure. It can be built in many shapes, such as the embodied tubular shape. The design allows operation on unstable ground, for example on ships. Flow of electrolyte can be regulated and directed in cascaded reactions by opening and closing the compartments of the outer or the inner electrodes using the provided electrode holders. The redox conditions inside the system can be controlled using off-the-shelf power supplies which are controlled using the provided algorithm. Gas collection can be regulated based on the level of liquid inside the system using the provided float switches or conductivity probes even as the system is moving or operated under zero-gravity conditions.