A flow cell system for an optical fluid analysis comprises a disposable flow cell having at least one flow chamber comprising a fluid pathway, and at least one pair of opposed light transmitting windows along the fluid pathway, an external flow cell holder for holding the flow cell, at least one light source, and an external detection device couplable with at least one of the flow cell holder and the flow cell for bringing the external detection device in optical communication with the flow cell, the device having at least one optical detection unit. The external detection device is configured to conduct optical measurements of the fluid that flows in the flow cell through at least one pair of windows from externally under illumination by the at least one light source.

Devices, systems, and methods can be used for the automated production of dendritic cells (DC) from dendritic cell progenitors, such as monocytes obtained from peripheral blood, and the automated generation of immunotherapeutic products from those dendritic cells, all within a closed system. The invention makes it possible to obtain sufficient quantities of a subject's own DC for use in preparing and characterizing vaccines, for activating and characterizing the activation state of the subject's immune response, and to aid in preventing and/or treating cancer or infectious disease.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A device for mechanically stabilizing a sensor port on a flexible bag including a rigid, substantially plate-shaped support part, which has a receptacle for a rigid connecting part integrated in the bag, in particular a sensor, and a holding mechanism on the receptacle. The device further includes a fastening element adapted to be attached to the holding mechanism of the support part such that it fixes the support part to the connecting part. The device may include a rigid support part which can be assembled from at least two parts, preferably struts. The parts surround a free area or an opening in which a rigid connecting part integrated in the bag can be placed. In an assembled state of the parts, the free area or the opening has a smaller diameter than the connecting part, so that the parts form a load-receiving mechanism for the connecting part.

A small-volume liquid container for dispensing a liquid medium, especially biological medium, into a bioprocessing system, especially into a bioreactor, wherein the liquid container provides an accommodation volume for the liquid medium, wherein the liquid container comprises two dimensionally stable container parts, which are movable relative to one another between an initial position and an end position, and an outlet connection for discharge of the liquid medium. It is proposed that the liquid container comprises a shape-changeable delimitation means which at least sectionally delimits the accommodation volume and which is connected to the dimensionally stable container parts in such a way that a relative movement of the dimensionally stable container parts to one another, causing a change in volume of the accommodation volume, is associated with a change in shape of the delimitation means.

A cell culture bag includes a body formed of a composite film including a first fluoropolymer and a second fluoropolymer. The body defines a cell culture compartment configured to hold a cell culture. The first fluoropolymer has a first thickness and the second fluoropolymer at least partially impregnates the first thickness of the first fluoropolymer. The composite film has a second thickness from 0.01 mm to 0.059 mm and a tensile strength in one direction of 10,000 psi to 92,000 psi. The composite film also has O.sub.2 flux of 2,000 cm.sup.3/M.sup.2/atm/day to 20,000 cm.sup.3/M.sup.2/atm/day and a total transmittance from 70% to 100%. A cell culture assembly includes a plurality of the cell culture bags connected in series. A cell culture container includes a cell culture compartment and a composite film connected to the cell culture compartment, the composite film including a first fluoropolymer and a second fluoropolymer.

A single-use chamber comprising a primary subchamber containing at least one primary component configured to perform an operation comprising at least one of generation and handling of a biological material; and a secondary subchamber containing at least one of a connection to equipment external to the single-use chamber and a secondary component, wherein the external equipment and the secondary component are configured to support the operation of the at least one primary component; wherein the primary subchamber and the secondary subchamber are configured to be connected to each other so that the at least one primary component is operatively coupled to at least one of the connection and the secondary component.

This cell production system is provided with: a robot that assists cell production; and a plurality of closed system cell production devices which are affected by the robot in a one-to-many manner, wherein the closed system cell production devices are each provided with a double-sided structure of a dangerous region side affected by the robot and a safe region side on the reverse side of the dangerous region side.

Systems for monitoring and controlling cell culture comprise a cell culture apparatus operably associated with a controller. The controller comprises a hardware processor coupled to memory containing instructions executable by the processor to cause the controller to receive data associated with cells to be cultured; connect to one or more databases to receive cell culture protocol data; and determine a cell culture protocol for the cells to be cultured. Methods of determining a cell culture protocol comprise receiving data associated with cells to be cultured; connecting to one or more databases to receive data about cell culture protocols; and determining a cell culture protocol for the cells to be cultured.

An extra-capillary fluid cycling unit for maintaining and cycling fluid volumes in a cell culture chamber includes a housing and a first flexible reservoir extra-capillary fluid reservoir disposed in the housing. The extra-capillary fluid reservoir is in fluid communication with a cell culture chamber. A second flexible reservoir is also located in the housing, the second flexible reservoir being in fluid communication with a pressure source. A sensor plate is movably disposed in the housing between the extra-capillary reservoir and the second reservoir, wherein the second reservoir is pressurized to move the sensor plate in relation to the extra-capillary reservoir to cause fluid cycling and maintain fluid volumes in the cell growth chamber.