An operation isolator forms an aseptic space. An incubator is connected to the operation isolator, in which cells are stored and cultured. A storage chamber stores articles used in the operation isolator. In order to carry articles from the outside into the storage chamber, a decontamination pass-box is provided. The storage chamber and the operation isolator are directly or indirectly connected to each other.

A cell culture dish, comprising a dish bottom, as well as an inner side wall and an outer side wall integrated with the dish bottom, wherein the inner part enclosed by the inner side wall forms a culture area, and a water tank is formed between the inner side wall and the outer side wall, and the water tank is located on the periphery of the culture area; at least one convex ring is provided on the surface of the dish bottom in the culture area, and the inner part enclosed by the convex ring forms a culture tank, wherein a number of culture microwells are provided at the bottom of at least one culture tank. Ultra-pure water is added to the water tank, and the evaporation of water creates a saturated humidity culture environment around the culture area, to prevent the risk of osmotic pressure increase caused by the evaporation of culture medium; convex rings are provided in the culture area, wherein culture medium is added to the culture tank formed by the convex ring, to make the droplets of the culture medium relatively fixed, and not easy to move to cause the loss of cells or the connection of droplets; culture microwells are provided inside the culture tank, to make the location of the cells limited in the microwells during the culture process, and the whole process can be observed or monitored with photographic apparatus, and cells of best quality can be selected out without disturbing the process of cell culture.

A device for controlling apical flow to a cell culture includes an apical insert that defines at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port. The apical insert includes a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, and a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert.

A container for ensuring the transport and the aseptic transfer of a biopharmaceutical product from or to a closed chamber provided with a leaktight joining device, includes: an annular flange delimiting an opening, a removable cover, built-in elements for locking/unlocking the removable cover and a peripheral envelope. The built-in locking/unlocking elements include a built-in functional locking/unlocking arrangement formed by a through-housing formed in the annular flange and a blind housing formed in the removable cover, and by a pin at an inner radial position and a pin at an outer radial position. The container is capable of being in an initial locking position, in an intermediate unlocking position and in a final locking position owing to the displacements of the pin at an inner radial position and the pin at an outer radial position in the blind hole of the removable cover and in the through-housing of the annular flange.

A fermenter can have at least one hollow fluid conduit disposed at least partially within a vessel. An external circumference of the hollow fluid conduit and an interior circumference of the vessel can define a downward flow path through which a multi-phase mixture including a liquid media and compressed gas substrate bubbles flows. An interior circumference of the hollow fluid conduit can defined an upward flow path which is in fluid communication with the downward flow path. The multi-phase liquid can flow through the upward flow path and exit the fermenter. Cooling may be provided in the hollow fluid conduit or the vessel. One or more backpressor generators can be used to maintain a backpressure on the fermenter. One or more fluid movers can be used to variously create an induced and/or forced flow in the downward and upward flow paths.

Disclosed herein are devices that include a top chamber including at least one port, a bottom chamber including at least one inlet and at least one outlet, wherein the opening of the at least one inlet is smaller than the opening of the at least one outlet, and a membrane located between the top chamber and the bottom chamber, wherein the membrane is fluidly coupled with the top chamber and the bottom chamber. Also disclosed herein are systems including the disclosed devices. The systems include liquid in one or more of the chambers of the device. Methods of using the devices and systems include producing a vacuum by flowing a liquid through the bottom chamber of the system. Due to the difference in size of the inlet and outlet in the bottom chamber, a vacuum is produced in the top chamber.

A bioreactor can include a plurality of unit cells. Each unit cell can include a floor configured to support a volume of liquid, the floor being symmetric across at least one axis of symmetry, and an injection port oriented at a center point of at least one axis of symmetry and configured to inject a fluid into the volume of liquid. The bioreactor can also include a peripheral side wall surrounding the plurality of unit cells such that the volume of liquid is retained in the bioreactor.

Systems and methods are provided for growing algae and/or other microorganisms in a controlled environment while reducing or minimizing the amount of energy required for maintaining desired conditions in the growth medium. The systems can be based on a photobioreactor having a “tube-in-tube structure”, where an outer cylindrical tube contains a heat regulation fluid that surrounds one or more inner cylinders that contain microorganisms in growth media. The heat regulation fluid in the outer cylinder, as well as the outer cylinder itself, can assist with regulating the temperature of the growth media in the inner cylinder(s).

The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall. The intermediate tray is positioned between the cover and the base tray, such that the gap region of the intermediate tray bottom plate is in alignment with the port located on the cover, resulting in the port, the intermediate tray and the base tray in fluid communication with one another which provides direct access, such as by a user to remove and/or add cells, cell media, and nutrients located on each of the intermediate and/or the base trays. Alternatively, the cell cultivating flask includes a plurality of intermediate trays stacked on top of one another and the gap regions of each intermediate tray are in alignment with each other and with the port on the cover.

The invention is an automated advanced tissue engineering system that comprises a housing in which one or more tissue engineering modules are accommodated together with a central microprocessor that controls functioning of the tissue engineering modules. In one embodiment, the tissue engineering module comprises a housing supporting one or more bioreactor chamber assemblies and a fluid reservoir operationally engageable with the housing. The bioreactor chamber assemblies may be selected depending on the end product option desired and may include, for example, a cell therapy bioreactor chamber, a single implant bioreactor chamber and a multiple (mosaic) implant bioreactor chamber.