Provided herein are injection molded perfusion-enabled bioreactors and systems including said bioreactors. The bioreactor can include a lid, a frame comprising an an-ay of sample wells, a membrane adhered to a bottom of the frame beneath the array of sample wells, and a skirt adhered to a bottom of the frame, such that the membrane is located between the frame and the skirt. The skirt can include a plurality of channels corresponding to the sample wells. When the sample wells are filled with a 3D cell culture support matrix, a pressure above the 3D cell culture support matrix is atmospheric pressure and a pressure below the membrane is less than atmospheric, thereby perfusing a fluid along a vertical fluid flow path from the sample wells through the

The present disclosure relates to a substrate apparatus (and methods of manufacturing the same) with one or more substrates having a substrate spacing for growing a cell mass. In particular embodiments, the disclosed substrate apparatus includes a substrate wound into a coiled configuration with an intra-coil spacing between coil layers. To provide the intra-coil spacing, certain implementations use a separator. For example, a separator is applied to a substrate surface, and the separator-substrate combination is wound together (e.g., around a spool). In turn, a locking element is attached to the substrate to maintain the coiled configuration. The separator is then removed via heat treatment, chemical treatment, or physical displacement—thereby leaving the intra-coil spacing between the coil layers. Alternatively, no separator is used to provide a substrate spacing. For example, in lieu of a separator, the locking element is actively applied to the substrate during the winding process.

A cell culture cartridge is provided comprising a plurality of zones geometrically configured to provide for symmetrical fluid flow with each of the plurality of zones to avoid dead areas in flow within each of the plurality of zones. In certain embodiments, at least eight inlets are provided, with an inlet positioned at each corner of the cell culture cartridge. In certain embodiments, a shared outlet is positioned on a top surface of the cell culture cartridge.

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

There is provided a bioreactor including: a chamber unit having an accommodating space therein; an upper fixing member and a lower fixing member that are disposed at respective planes perpendicular to a central axis of the chamber unit inside the chamber unit and are movable in a central-axis direction of the chamber unit; and a filling layer that is formed in a space between the upper fixing member and the lower fixing member inside the chamber unit and contains a plurality of carriers.

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

A structure for culturing cells includes growth medium regions on a surface of the structure. Each of the growth medium regions includes a growth medium surface configured to receive and promote growth in a cell that is being cultured. The structure includes a non-growth medium. The non-growth medium includes a non-growth medium surface configured to receive the cell that is being cultured.

A device (20) for creating cell-exclusion zones comprises a baseplate (21) having a first major face from which a plurality of exclusion reliefs (22) project, each exclusion relief (22) having at least one distal end (23) configured for contact with a bottom (11b) of a respective well (11) of a multi-well cell-culture plate (10). The baseplate (21) and the plurality of exclusion reliefs (22) are defined at least in part by an elastic or flexible material in such a way that at least the baseplate (21) is elastically deformable at least during removal thereof from a multi-well cell-culture plate (10), the elastic or flexible material preferably being an elastomer.

A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

The invention inter alia pertains to methods and kits for analysis of one or more cell released biomolecules. Furthermore, the invention relates to a plurality of sequenceable products comprising different sequence elements. The described technology is useful for a variety of applications, in particular biomolecule analysis applications, e.g. for obtaining biomolecule release profiles of single cells in a multiplexed manner, wherein the cells are provided in a matrix