An automatic culture device includes: a ventilation adapter configured to supply, in a state in which a culture vessel configured to culture cells is placed thereon, a gas containing CO.sub.2 to the culture vessel; a gas supply unit configured to supply the gas containing CO.sub.2 to the ventilation adapter; and a gas holding space forming portion configured to airtightly hold the gas containing CO.sub.2 supplied from the gas supply unit when the culture vessel is placed at a predetermined position of the ventilation adapter, an upper portion of the gas holding space forming portion directly or indirectly supporting at least a part of a gas exchange membrane provided on a lower side of the culture vessel, and a lower end portion of the gas holding space forming portion coming into contact with at least a part of an upper surface of the ventilation adapter.

Process for producing biogas from a digestate obtained from a digester, said process comprising a step of recovering the digestate at the outlet of a digester, a post-digester or a storage tank; a step of separating the digestate into a solid digestate and a liquid digestate; a step of introducing the solid digestate into at least one closed tank; a step of anaerobic digestion in the tank with neither heating nor mixing; and a step of recovering the biogas at the tank outlet.

Embodiments relate to a bioprocessing system having a main line for conduction of a main flow of a liquid medium, having a main flow pump and having a system part connected to the main line. The bioprocessing system comprises a powder transfer system and a feed line which is connected to the powder transfer system and which opens into the main line and which comprises a return flow preventer for prevention of a return flow of liquid medium, the powder transfer system feeds the additive into the feed line in powder form and drives it toward the feed point by means of a pressurized transport gas and at least some of the fed additive is input into the liquid medium within the main line downstream of the feed point before the liquid medium enriched with the additive passes through or reaches the system part connected to the main line.

Bioreactors including bioreactors having features for improved performance, sensing and ease of use. Many embodiments provide bioreactors having improved dip-tubes, magnetically coupled agitators, condensers and sensing ability. Particular embodiments provide a bioreactor including a vessel having an inner volume for liquid contents and a head plate (HP) for coupling a plurality of components to the bioreactor where the HP is coupled to a top portion of the vessel and includes a plurality of ports. A diametric magnetic (DM) drive assembly (DMDA) and agitation shaft (AS) including at least one impeller are rotatably coupled to the HP. The DMDA and AS are rotated by non-vertical magnetic forces from a rotating DM positioned above the HP. A dip tube assembly (DTA) having a plurality of inner fluidic channels is positioned through a HP port such that a DTA end extends into the vessel for delivery liquids and sparging gasses.

Aspects and embodiments relate to a biological sample culture unit, a biological sample culture module comprising at least two biological sample culture units and culturing apparatus including a biological sample culture unit or biological sample culture module. Aspects and embodiments also provide methods to culture biological samples within a biological culture unit, biological culture module or biological culture apparatus. All aspects utilise a biological sample culture unit comprising a first chamber configured to accommodate a biological sample, culture medium and a gas reservoir; a second chamber configured to accommodate a reservoir of culture medium and a further gas reservoir, the second chamber comprising a gas port configured to couple the further gas reservoir to a gas source; an inlet conduit linking the second chamber with the first chamber configured to allow flow of fluid between the second chamber and the first chamber in dependence upon a pressure difference between the gas reservoirs in the second chamber and the first chamber. Aspects support provision of a small footprint biological sample culture environment which is highly scalable, which can sustain a culture environment with controllable conditions for an extended period and in which maintenance of, and testing a biological sample within, the culture environment can be automated, thus facilitating high throughput, minimal disruption to biological samples under study and improved reproducibility.

Disclosed is a process and system for generating hydrogen from carbon dioxide. The process and system for generating a hydrogen gas stream from a carbon dioxide gas stream comprises converting a first waste carbon dioxide gas stream to an organic feedstock using an algal source in a photosynthesis step. The organic feedstock is then converted using an organism to the hydrogen gas stream and gaseous by-products in a biodecomposition step. The generated hydrogen gas may then be collected.

The present disclosure is directed to a system for anaerobic dry digestion of lignocellulosic biomass including: a dry digester having a ground sheet and a cover for the lignocellulosic biomass. The digester generates leachate and biogas from anaerobic digestion. The system includes an irrigation/port system for wetting of the lignocellulosic biomass under the cover; a sump system for monitoring the leachate; a rotary valve-connected at least to the pump and the dry digester; a gas storage bladder for collecting the biogas, the gas storage bladder connected at least to a gas treatment unit and a gas booster pump; and a hi-rate digester tank connected at least to the rotary valve for receiving the leachate therefrom.

This application discloses a device and a method for culturing Geobacter that produces ultra-high conductivity bio-nanowires. The device comprises a tank, at least two liquid color sensors, at least two dissolved oxygen sensors, a liquid level sensor, and a stirring paddle. The top of the tank is provided with an air valve; an upper part of the tank is provided with a feed inlet, a lower part is provided with a culture medium outlet, and the bottom of the tank is provided with a discharge outlet. This application can recycle ferrous citrate, add substrates multiple times, cultivate Geobacter that grow ultra-high conductivity bio-nanowires in batches, and achieve the amplification and cultivation of a large number of Geobacter metallireducens in the same volume.

A bioreactor (1, 2, 3) and use thereof for converting organic residual and/or waste materials into an organic nutrient solution with a proportion of at least 10% plant-available mineralized nitrogen relative to the total nitrogen content of the nutrient solution. A process for preparing an organic nutrient solution is also provided, as well as an organic nutrient solution, use of an organic nutrient solution as an absorbent for carbon dioxide storage, use of an organic nutrient solution as an agent for binding carbon in plants and soils and to a nutrient production and carbon dioxide storage system.

A culture container for culturing epithelial cells, includes an upper container, a lid member configured to airtightly fit with an opening portion of the upper container, and a lower container configured to accommodate the upper container and a cell culture medium, in which at least a part of an area of the upper container in contact with the cell culture medium is formed of a membrane that is permeable to at least a part of components of the cell culture medium and impermeable to a cell, and a material of the lid member has an oxygen permeability coefficient of 1.010.sup.6 cm.sup.3 cm/(cm.sup.2.Math.sec.Math.atm) or less.