The invention provides a device for growing cells—referred to as a cassette. The cell culturing device includes a housing that contains a lid having an optically clear window; a fluid distribution channel; a sample injection port fluidically connected to the fluid distribution channel; a base housing a porous media pad; and a media injection port fluidically connected to the media pad. The lid mates to the base to form a sterile seal; the fluid distribution channel is disposed over the media pad, which is viewable through the optical window; and sample fluid introduced into the fluid distribution channel is distributed evenly to the media pad, e.g., via a plurality of channels. The invention also provides kits that include cassettes of the invention and a tube set.

A container with an embedded structure mainly includes: a first plate with a first plate body, a first end surface located on one side of the first plate body; a second plate with a second plate body, a second end surface opposite to the first end surface and located on one side of the second plate body, a flexible protrusion is annularly protruded on the second end surface and abuts against the first plate to form an accommodating space between the first end surface, the second end surface, and the protrusion for a fluid to flow in the accommodating space, and the accommodating space has an accommodating height between the first end surface and the second end surface; and a shape of a portion of a free end of the protrusion abutting the first end surface is deformed.

The present invention provides a parallel bioreactor system, comprising: an oscillator for generating oscillating motion; a plurality of culture vessels mounted on the oscillator, wherein each culture vessel is provided with an inner cavity, the inner cavity comprises a cylindrical portion at the upper part and an inverted truncated conical bottom at the lower part, a cross section of the cylindrical portion is consistent with the cross section of the top of the inverted truncated conical bottom, and the bottom of the cylindrical portion is joined with the top of the inverted truncated conical bottom; disposable culture bags arranged in the inner cavities of the culture vessels and used for accommodating culture solution, wherein each disposable culture bag is provided with a multifunctional cover plate, and the multifunctional cover plate is connected to the top of the culture bag to seal the culture bag, and is provided with a plurality of connection holes leading to interior of the disposable culture bag; and a control system, wherein the control system controls the oscillating motion of the oscillator and parameters of the culture solution in the disposable culture bags.

A system for quickly determining antibiotic sensitivity of a microorganism comprising a triangular-shaped plate and cover for culturing, recovering, and re-suspending recovered microbial colonies and a second triangular-shaped plate comprising a non-liquid medium cut with concentric trenches over which the re-suspended microbial colonies are distributed by centrifugation and contacted with antimicrobial strips or disks.

Methods and apparatus of bioreactors for therapeutic cells manufacturing are provided herein. In some embodiments, a bioreactor includes: an upper bioreactor reservoir configured to perform multiple cell therapy manufacturing process steps including genetic modification and expansion to a plurality of cells disposed therein, wherein the upper bioreactor reservoir includes a plurality of ports for delivering fluids into and out of the upper bioreactor reservoir; a lower bioreactor compartment configured to hold a suspension comprising a molecular species; and a membrane disposed between the lower bioreactor compartment and the upper bioreactor reservoir, wherein the membrane includes a plurality of micro-straws extending through the membrane and into the upper bioreactor reservoir to transfect the plurality of cells with the molecular species.

A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

An apparatus for draining a bioreactor vessel includes a tubular body portion having an interior passageway, and at least one aperture in the tubular body portion providing for fluid communication with the interior passageway, the tubular body portion being configured for positioning at a bottom of a vessel, and a suction tube having a first end configured for fluid coupling with the tubular body portion, and a second end configured for fluid coupling with a port in a sidewall of the vessel.

A cell culture container includes: a container body; a cell culture insert having a tubular portion and an oxygen-permeable membrane; and a lid member. The container body is provided with an opening that communicates with an interior of the container body. The tubular portion includes an upper end and a lower end, and is inserted in the opening such that the lower end is located inside the container body. The lower end side of the tubular portion is closed by the membrane. The upper end side of the tubular portion is closed by the lid member. The lid member includes a lower surface that faces toward an interior of the tubular portion. The lid member is provided with an electrode insertion port and a culture medium outlet port that each communicate with the interior of the tubular portion at the lower surface.

A cell culture method and a cell culture device used with the cell culture method allow mass-producing a three-layer blood-brain barrier (BBB) in vitro model including a vascular endothelial cell, a pericyte, and an astrocyte. A cell culture method enhances mass production of the three-layer BBB models. A cell culture method includes injecting a suspended cell culture medium into an outer surface of a porous membrane in a cell culture insert, seeding the cell culture medium with an astrocyte to culture the astrocyte, seeding the cell culture insert being inverted with a pericyte to culture the pericyte, seeding the cell culture insert with a temperature-sensitive vascular endothelial cell embedded in a temperature-sensitive gel to culture the vascular endothelial cell, and melting the temperature-sensitive gel.

A columnar cell culture vessel is disclosed. The columnar cell culture vessel according to an embodiment of the present disclosure may include: a cylindrical body having a chamber disposed therein, an inlet disposed through one side in an axial direction, and an outlet disposed through in the axial direction; a first cap coupled to the inlet and having an inflow port disposed therethrough; a second cap coupled to the outlet and having a discharge port disposed therethrough; and a support which is filled in the chamber and to which cells are attached to be cultured, wherein a culture solution is introduced into the chamber through the inflow port and discharged to the outside of the chamber through the discharge port.