A container for storing, mixing and/or cultivating a medium, in particular a bioreactor for a medium, comprises a line system as a discharge line, feed line and/or bypass line of the bioreactor. The system can comprise a line body formed as a single piece for a medium to flow therethrough. The has a first and a second connection region for connecting in particular to the container and/or the line system and at least a first and a second coupling apparatus in the region between the connection regions.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

The invention relates to a carrier which is suitable for the cultivation of cells and is preferably a plate-shaped carrier, e.g. made of glass, silicon or plastic or a combination thereof, in which carrier at least one first recess is formed which extends over a first thickness portion of the carrier, wherein an array of second recesses extends from the first recess into a second thickness portion of the carrier adjacent to the first thickness portion. The first thickness portion may have a thickness of a few micrometres to several centimetres. The second recesses, which extend from a first recess into the second thickness portion and each form a second array, form cups which are suitable for receiving cells and/or synthetic particles, e.g. made of plastic or glass.

Digital microfluidic (DMF) methods and apparatuses (including devices, systems, cartridges, DMF readers, etc.), and in particular DMF apparatuses and methods adapted for large volume. For example, described herein are methods and apparatuses for DMF using an air gap having a width of the gap that may be between 0.3 mm and 3 mm. Also described herein are DMF readers for use with a DMF cartridges, including those adapted for use with large air gap/large volume, although smaller volumes may be used as well.

The present invention is for a closed environment process for the growth and differentiation of cells and the culturing of cells to confluency for the production of tissue. The tissue may be a clean meat product.

An in vitro tissue plate may include a well plate, a fluidic plate disposed on a bottom surface of the well plate, and a media manifold disposed on a bottom surface of the fluidic plate. The well plate may have at least two wells, including a tissue well and a waste well. The fluid plate may include a fluid channel extending between and fluidly connecting the tissue well to the waste well. The media manifold may include a one or more media outlets fluidly connected to the fluid channel. A tissue layer may be deposited in the tissue well. The tissue layer may include human cells such as neurovascular cells.

A substance introduction unit, used for introduction of a substance into a cell by electroporation, includes an accommodation container configured to accommodate a cell suspension containing the cell and the substance; and a pair of electrodes which has electrode surfaces exposed to an internal space of the accommodation container and is configured to apply a voltage to the cell suspension accommodated in the accommodation container, the accommodation container including a cell-impermeable member configured to partition the internal space of the accommodation container.

An alga growing apparatus that includes a gas dissolving portion, an alga tank, first and second LEDs, a supplying portion, and a circulation pump portion. Gas dissolving portion dissolves carbon dioxide and oxygen into deep-ocean water to form growing water. The circulation pump portion sucks out and delivers the growing water and the nori thalli from the alga tank 30 to the gas dissolving portion and injects the growing water and the nori thalli into the alga tank upon passage through the gas dissolving portion and a supply pipe of the supplying portion. The supplying portion discharges the growing water and the nori thalli along a direction obliquely intersecting a curving direction of an inner side surface of the alga tank and the growing water flows inside the alga tank as an eddy flow.

A flow distribution device for bioprocess systems, comprising: ⋅—a flow distribution manifold (12; 112; 212; 312) comprising: ⋅o at least four fluid connection tubes (14), wherein each fluid connection tube (14) comprises a first end (18) for fluid connection and an opposite second end (20) ⋅o a central common compartment (30; 130; 230; 330) to which the second ends (20) of each of the fluid connection tubes (14) are connected, whereby the first ends (18) of each of the fluid connection tubes (14) can be in fluid communication with the central common compartment (30; 130; 230; 330) ⋅—at least three pinching members (41) which are provided in connection with one fluid connection tube (14) of the flow distribution manifold (12; 112; 212; 312) each, wherein each of said pinching members (41) can be controlled into at least a first and a second position, wherein in the first position for each of the pinching members (41) the pinching member pinches one of the fluid connection tubes (14) such that fluid flow is prevented between the first end (18) and the second end (20) of this fluid connection tube (14)