Tissue culture platforms that may be configured for tissue culture or biological cell culture, and methods for use thereof, are described. In general, the tissue culture platforms include multiple well chambers that are fluidically coupled by one or more channels Flow between the different well chambers is controlled via one or more selector valves, enabling a single tissue culture platform that can provide multiple integrated culture subsystems, multiple non-interacting culture subsystems, or combinations thereof.

An alga growing apparatus that includes a gas dissolving portion, an alga tank, first and second LEDs, a supplying portion, and a circulation pump portion. Gas dissolving portion dissolves carbon dioxide and oxygen into deep-ocean water to form growing water. The circulation pump portion sucks out and delivers the growing water and the nori thalli from the alga tank 30 to the gas dissolving portion and injects the growing water and the nori thalli into the alga tank upon passage through the gas dissolving portion and a supply pipe of the supplying portion. The supplying portion discharges the growing water and the nori thalli along a direction obliquely intersecting a curving direction of an inner side surface of the alga tank and the growing water flows inside the alga tank as an eddy flow.

There is disclosed a fluid distribution system for distributing fluid from a single source to a plurality of downstream receptacles. The system has a distribution manifold with a single inlet and a plurality of outlets arrayed around a circumferential outer periphery. The outlets may be directed to the different receptacles which each have their own vent filter, or each receptacle connects back to the distribution manifold for common venting.

The present invention provides a system and a method for the production of cells and/or cell products. The system comprises at least one cell culture unit comprising at least one bioreactor for culturing cells, at least one technical control unit for controlling a cell growth parameters, said technical control unit is at least fluidly connected to the cell culture unit, and at least one air treatment unit for treating ambient air, said air treatment unit is fluidly connected to the cell culture unit. The system is characterized in that the system is autonomous and the bioreactor total volume is at most 1000L.

A bioreactor system for conditioning of pluripotent cells or cell media is provided. In further aspects, conditioned pluripotent cells and methods for making such cells are provided.

The invention relates to a device for incubating cultures, comprising a culture chamber storing the cultures, the cultures being fumigated with an incubation gas. The invention is characterized in that the incubation gas source is used when incubating the cultures with the incubation gas source which is free of liquid incubation gas.

The invention relates to a bioprocessing system having a main line (2) for conduction of a main flow (3) of a liquid, in particular biological, medium (45), having a main flow pump (5) for generation of the main flow (3) and having a system part (46) connected to the main line (2). What is proposed is that the bioprocessing system (1) comprises a powder transfer system (8) for metered delivery, in particular dust-free metered delivery, of an additive (47) and a feed line (9) which is connected to the powder transfer system (8) and which opens into the main line (2) at a feed point (10) and which comprises a return flow preventer (11) for prevention of a return flow of liquid medium (45), that the powder transfer system (8) feeds the additive (47) into the feed line (9) in powder form and drives it toward the feed point (10) by means of a pressurized transport gas (12) and that at least some of the fed additive (47) is input into the liquid medium (45) within the main line (2) downstream of the feed point (10) before the liquid medium (45) enriched with the additive (47) passes through or reaches the system part (46) connected to the main line (2).

A bioreactor may comprise a head-plate configured to provide coupling features to attach selected components to the bioreactor. The bioreactor may also comprise impeller and sparger, and the sparger is positioned at a pre-determined distance from the impeller based at least in part on a spatial configuration of the head-plate. The bioreactor further comprises a vessel defining a hollow enclosure for receiving a culture medium, and an opening of the vessel is covered by the head-plate.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Some aspects of the disclosure are related to systems and methods for controlled oxygen release from biomaterials in vessels and unit operations or components of cell culture, cell containment, and/or bioreactor. Vessels, unit operations, devices, and/or components of the invention may be used to perform all or part of a biological and/or chemical process involving biologicals (e.g., a plurality of cells) in the presence of oxygen-releasing agents. In some embodiments, a system comprises a vessel comprises an oxygen-releasing agent configured to generate in-situ and release oxygen in a sustained manner. The presence of an oxygen-releasing agent may advantageously allow for high cell density fermentation and cell cultivation in a vessel and provide an alternative for supplemental gassing means (e.g., sparger, etc.). Some embodiments of the disclosure are directed to employing the oxygen-releasing agents in microfluidic or millifluidic systems.