Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

A bioreactor can include: a tissue culture chamber; at least one inlet port into the tissue culture chamber; an inlet port member located in each inlet including an inlet tube extending into the tissue culture chamber; at least one outlet port into the tissue culture chamber; an outlet port member located in each outlet including an outlet tube extending into the tissue culture chamber; an optical cover; and a hydrogel can be located in the tissue culture chamber having at least one lumen fluidly coupling the inlet tube to the outlet tube, wherein an inlet interface region of the hydrogel is constrained around the inlet tube and an outlet interface region of the hydrogel is constrained around the outlet tube.

A cell culture apparatus is provided. A cell culture apparatus according to an exemplary embodiment of the present invention comprises: a housing having an internal space which is filled with a culture medium for cell culture; and a support assembly which is provided in a plate form having a predetermined area, comprises a plurality of supports provided in multiple stages at a certain distance apart from each other in one direction, and is inserted in the internal space in the state where the plurality of supports are coupled to each other via at least one fastening bar.

The present invention relates to a computer implemented method for controlling a fluid coupling network (170), the fluid coupling network (170) being configured to be fluidly couplable to bioreactor (110), a gas source (120), a buffer source (140), a waste port (150), a processing system (160) and a conduit reservoir (208), the fluid coupling network (170) comprising a barrier unit (220) configured to sterilely separate fluid paths within the fluid coupling network (170), the fluid coupling network (170) being controllable to sample the bioreactor (110), the method comprising obtaining (510) a fluid sample from the bioreactor (110), wherein the step of obtaining the fluid sample comprises controlling a flow of fluid from the bioreactor (110) via the conduit reservoir (208) and the fluid coupling network (170) to the waste port (150), to fill the conduit reservoir (208) with the fluid from the bioreactor (110), providing (530) the fluid sample, wherein the step of providing the fluid sample comprises to provide the fluid sample to the processing system (160) from the conduit reservoir (208) via the fluid coupling network (170), and returning (540) residual fluid sample to the bioreactor (110), wherein the residual fluid sample is comprised by a part of the fluid coupling network (170) separated by the barrier unit (220), wherein the step of returning residual fluid sample comprises controlling a flow of gas from a gas source (120) to the bioreactor (110) via the part of the fluid coupling network (170).

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

A system and method for producing cellulosic ethanol by biomass saccharification and fermentation is provided. The system includes a first reactor configured to carry out an enzymatic hydrolysis reaction of a biomass feedstock, a second reactor configured to carry out a fermentation reaction of the biomass feedstock, and a collection tank configured to collect the cellulosic ethanol. The first reactor and second reactor are provided with an enzymatic hydrolysis dosing pipe and a fermentation dosing pipe, respectively. An outlet of the first reactor is connected to an inlet of the second reactor through a fermentation pipe. An outlet of the second reactor is connected to an inlet of the first reactor through a discharge pipe that is provided with a first discharge pipe configured to discharge material from the second reactor. The first reactor is connected to an inlet of the collection tank through an ethanol pipe.

A fluid distribution system includes single use distributor manifolds each having a distributor manifold inlet and a plurality of distributor manifold outlets in fluid communication with the distributor manifold inlet. A set of single use filler manifolds can be sequentially connected to a respective one of the outlets of a single use distributor manifold immediately upstream of the connection point of the set of single use filler manifolds. In embodiments, an upstream distributor manifold can be used to feed fluid to a set of intermediary distributor manifolds where the set of intermediary distributor manifolds corresponds to the number of outlets of the upstream distributor manifold.

A cell culturing system includes a reactor, a flow channel that allows a culture medium to flow into and out of the reactor, a waste liquid channel that discharges the culture medium from the flow channel, and a waste liquid container capable of storing the culture medium passing through the waste liquid channel. The waste liquid channel includes a temporary storage unit capable of temporarily storing the culture medium. The waste liquid container is located below the temporary storage unit) in a gravity direction, and the temporary storage unit is provided above the flow channel in the gravity direction. The temporary storage unit temporarily stores the culture medium discharged from the flow channel and allows the culture medium to flow out toward the waste liquid container.

A system for culturing cells includes a bioreactor including a scaffold on which the cells tend to adhere. The system further includes a circulatory system that intermittently flows fluid over the scaffold.