fermelnters or other processes for the treatment or conversion of organic substances, and also for improving viscosity, in which specifically required, technological or biological procedures are combined in one mixing and combi-hydrolysis tank. The device has one or more descending walls for separating the gas chamber in the top part of the tank, one descending wall of which separates the gas chamber of the tank opening from the other tank, and optionally a further descending wall, the lower end of which is permanently located above the lowest fluid level and which separates the gas chamber of the pump chamber from the other tank. The device optionally has an ultrasonic module for treating a recirculated material from an advanced fermentation stage or from the device according to claim 1, and also a gas supply system in the lower zones of the feed and/or pump chamber for hydrogenous gases, produced in the process or supplied externally, for stimulating methane formation by means of hydrogen-oxidising archaea.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.

Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N−1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N−1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.

A fluid module having a valve node is specified, which includes a base body delimited by an outer surface and having a central fluid space, and at least four fluid channels which are fluidically separated from each other and each open into the central fluid space, wherein at least two valve connections are provided on the outer surface, and wherein a coupling point is provided on the valve node, to which a coupling module is fastened, in which a connection channel is formed, which is fluidically connected to one of the fluid channels in the valve node, and wherein the coupling module includes a coupling valve which selectively unblocks and closes the connection channel. A fluid system having at least two fluid modules, and a method of cleaning a fluid system or a fluid module are furthermore specified.

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

The invention relates to the production of biogas by means of multi-stage fermentation in a mono-tank. The device for biogas production by means of multi-stage fermentation in a single tank according to the invention is characterised in that the inside of the tank is provided with additional partitions, which are arranged in such a manner that the total volume of the tank is divided into at least two or more partial volumes, and partitioned tank sections are thus present, in which the various fermentation stages occur and which can be controlled according to the actual reaction progression. The device optionally has an ultrasonic module for treating a recirculated material from an advanced fermentation stage or from the device according to the invention.

Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.

The invention relates to a modular, mobile, compact, multi-stage and highly efficient biogas facility, a method for operating a modular biogas facility, and a system for the computer-assisted, decentralized monitoring and control of at least one modular biogas facility. The system can be equipped with modular, local intelligence and a local control unit. The modular biogas facility is provided with a plurality of tanks for accommodating biomass. The tanks can be fluidically connected to one another. Furthermore, at least one gas reservoir is provided for the biogas produced in the modular biogas facility. Each of the tanks is a module in the biogas facility. Each tank can be positioned in a rigid and cuboidal frame, with the cuboidal frame having six side faces. The side faces of the cuboidal frame define an envelope for the tank.