A device and a method for clinically providing a preparation of autologous phages, namely, those that can verifiably be traced back to originating from a very specific person and preferably are also only intended for use in this one specific person includes a phage culturing device which is a fluid line system that is sealed off with respect to the outside environment The phages in the fluid line system obtained after at least one-time culturing are separated from bacteria by way of filtration, and preferably by way of tangential flow filtration. The phages separated by way of filtration are transferred into a collection vessel that is connected to the fluid line system and are preferably removed from the fluid line system, using the collection vessel, as a usable, preferably autologous preparation.

Provided are: a production device by which a cell structure having a three-dimensional structure is produced using a plurality of linear members; a production system therefor; and a production method therefor. The production device 100 comprises a top plate 110, pins 120A to 120D, a first slide plate 130, a second slide plate 140, a stopper 150, a base plate 160, an outer peripheral needle-shaped member 170 and an inner peripheral needle-shaped member 180. Cell aggregates 400 are put into a three-dimensional tubular space S1 that is defined by the outer peripheral needle-shaped member 170 and the inner peripheral needle-shaped member 180. Then, the top plate 110 is pressed downward on the accumulated cell aggregates 400. Thus, the cell aggregates 400 are immersed in a culture solution 210 and stuck together so that a tubular cell structure 500 is produced using the three-dimensional space S1 as a mold.

A fluid distribution system includes single use distributor manifolds each having a distributor manifold inlet and a plurality of distributor manifold outlets in fluid communication with the distributor manifold inlet. A set of single use filler manifolds can be sequentially connected to a respective one of the outlets of a single use distributor manifold immediately upstream of the connection point of the set of single use filler manifolds. In embodiments, an upstream distributor manifold can be used to feed fluid to a set of intermediary distributor manifolds where the set of intermediary distributor manifolds corresponds to the number of outlets of the upstream distributor manifold.

A fluid distribution system includes single use distributor manifolds each having a distributor manifold inlet and a plurality of distributor manifold outlets in fluid communication with the distributor manifold inlet. A set of single use filler manifolds can be sequentially connected to a respective one of the outlets of a single use distributor manifold immediately upstream of the connection point of the set of single use filler manifolds. In embodiments, an upstream distributor manifold can be used to feed fluid to a set of intermediary distributor manifolds where the set of intermediary distributor manifolds corresponds to the number of outlets of the upstream distributor manifold.

A genetically modified cell that can be mass-produced efficiently. The genetically modified cell production system includes a cell-processing isolator for seeding a T cell in a well plate (culture vessel) a cell culture incubator (cell storage) for holding the culture vessel in which the T cell has been seeded and culturing the T cell a virus-processing isolator (nucleic acid preparation isolator) for providing, to the well plate, a virus having a nucleic acid containing a gene to be introduced into a cell a virus storage (nucleic acid storage) for holding the well plate from the virus-processing isolator and a virus infection isolator (nucleic acid introduction isolator) for introducing the nucleic acid into the T cell by infecting the T cell with the virus.

A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.

An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.

The invention relates to the production of microalgae biomass. The microalgae contained in a suspension of water and microalgae are continuously phototrophically or mixotrophically cultivated in a cultivation module (1), which is passed multiple times by the suspension and has a gas part and a liquid part with a liquid supply (3), by supplying light from at least one artificial light source (5) and nutrients. According to the turbidity established by sensors, volume fractions of the suspension are repeatedly discharged from the cultivation module (1) for the harvest of microalgae and removed by means of a centrifuge (7). The cultivation of the microalgae occurs in an climate chamber forming the cultivation module (1), which is operated using water. Alongside a regulating of the temperature of the suspension, there also occurs a regulating of its pH value via the controlled addition of buffer ions and a regulating of the redox potential of the suspension and thereby also of its microbial contamination by controlling the light and nutrient supply, as well of a metered addition of oxygen. In addition, after the removal of microalgae, the remaining suspension is irradiated with UV light in order to kill unwanted microbial contamination before being returned into the cultivation module (1).

Disclosed herein is an instrument suitable for processing cells for example culturing, concentrating or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump; and a disposable processing kit complementary to the mechanical elements within the housing and comprising a fluid circuit including a fluid reservoir and plural fluid paths capable of carrying fluid flow caused by said pump(s), the instrument further including a mechanism for determining the quantity, or change in quantity of the fluid in the reservoir resulting from said fluid flow, the instrument yet further comprising a controller operable to control at least the pump and operable to perform a fault determination process, which includes the steps of determining the expected flow rate of said pump(s) calculated from the speed of the pump(s) and comparing that expected flow with the change in quantity of the fluid in the reservoir as determined by said mechanism.

There is provided a connector, for introducing or extracting a material to or from at least one receptacle, comprising a housing extending between a distal end and a proximal end, the housing comprising, at least at one end, a pierceable seal; a hollow needle mounted, at least partially, within the housing between the distal end and the proximal end of the housing, a first end of the hollow needle being connected or connectable to a first corresponding receptacle, and a second end of the hollow needle facing the pierceable seal at an end of the housing; and an actuating mechanism acting on the housing or the hollow needle to enable the hollow needle to pierce the pierceable seal thereby forming a communication through the pierceable seal, such that material is able to transfer through the connector.