Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

A perfusion module and a perfusion culture system are provided, including: a bioreaction module, including at least one bioreactor; a perfusion module, including at least one perfusion chamber, each perfusion chamber is connected to a pressure regulating pipe for inflow or outflow of an airflow; when there are multiple perfusion chambers, every two adjacent perfusion chambers are connected to each other; at least one bioreactor, the perfusion module, the filtration module are communicated in sequence, if there are more than two perfusion chambers, all of perfusion chambers are set side by side; a cell culture fluid in at least one bioreactor flows into at least one perfusion chamber, flows out from at least one perfusion chamber into at least one filter, and a permeate from at least one filter flows into a harvesting device. The present disclosure avoids high shear forces in the perfusion culture system.

Electrochemical sensing devices and methods of using thereof employs a set of one or more sensor-integrated sampling wells or containers that operates with a pressure differential micro-valve to move controlled volume of sampled fluid within a controlled cell-growing environment. The differential micro-valve can be integrated into an instrumented well having one or more sensors to provide a high-throughput smart well plate platform for use in automation operation in diagnostics and drug discovery.

The bioreactor can use a number of different culture growing structures for culturing cells including a lattice structure and a support matrix structure. The culture growing structure whether it be a support matrix using wicking and/or a lattice structure may be coated with a thermal responsive polymer. The material of interest growing on the culture growing structure can be removed by changing the temperature that the thermal responsive polymer on the surface of the culture growing structure is exposed to and thus in some cases release the thermal responsive polymer along with the material of interest from the remainder of the culture growing structure.

A system for growing algae includes a photobioreactor comprising a tubular structure having inner and outer surfaces, an annular space defined between the inner and outer surfaces, an inlet to allow an algae slurry to enter the annular space, and a mechanism configured to produce radially-directed contractions and expansions in the tubular structure. At least one of the inner and outer surfaces is transparent to at least some wavelengths of light useful for growing algae within the algae slurry.

A bioreactor includes a reservoir container for holding a liquid medium, a duct providing a flowpath in a generally vertical direction upward from the reservoir container, a plurality of fiber assemblies located within the duct, a top of which is higher than a top of the plurality of fiber assemblies, and a circulation system. The upper end of the duct comprises an overflow wall surrounded by a moat, a bottom of which is lower than a top of the overflow wall. The upper end of the duct and moat contact a pocket region that is bounded by a structure that is connected to the duct and that is isolated from fluid communication with an exterior of the pocket region. The liquid medium flows over the overflow wall within the pocket region, contacts gas in the pocket region, overflows into the moat and is removed therefrom by the circulation system.

The present disclosure provides a fluid delivery consumable for delivering a fluid dose to a bioreactor. The fluid delivery consumable comprising a vial for holding the fluid dose, the vial having an outlet and an open end opposite to the outlet, a plunger engaged with the open end and operable to urge the fluid dose toward the outlet, and a connector proximal to the outlet, the connector being attachable to the bioreactor and adapted to move the fluid dose from the vial to the bioreactor based on operation of the plunger.

A multi-connector port provides an air connection for sterile air, at least one access arranged on a connecting piece, and at least one line arranged on the connecting piece. The access comprises a self-closing diaphragm.

Microfluidic device comprising at least one cell culture unit for forming, culturing, growing and/or maintaining a 3D tissue structure such as a 3D strip of cardiac tissue, wherein the at least one cell culture unit comprises: a respective culture chamber for culturing cells having a chamber outlet opening; and a cell supply channel arranged to guide a microfluidic flow of liquid holding cells between a channel inlet and a channel outlet, wherein the cell supply channel is provided with a flow inhibitor which is operable to selectively provide a flow inhibiting state or a flow permitting state depending on a fluid pressure at the flow inhibitor, wherein, in the flow inhibiting state, the flow inhibitor is configured to substantially inhibit liquid flow between the cell supply channel and the culture chamber, wherein, in the flow permitting state, the flow inhibitor is configured to permit such liquid flow such that the cell supply channel is in liquid communication with the culture chamber to supply the culture chamber with cells, wherein the culture chamber is provided with at least two mutually spaced apart elastic support structures which extend in the culture chamber and which are configured for elastically supporting a tissue formed in the culture chamber, in particular a cultured 3D tissue formed from the cells, wherein the elastic support structures are elastically deformable, in particular flexible, in particular to vary a mutual distance of said support structures under influence of a varying contraction force between said support structures.