Provided is a biological fluidic system that provides a high degree of sterilization by performing the biological processing in a closed system in which the fluid is contained in chambers that are isolated from the environment in a manner that does not permit ingress of contaminants.

The present invention relates to a fungible suspension cell culture device. In particular, the invention relates to a fungible device with a configuration which allows modifying the volume of the cell culture area by automatically transferring said cell culture between different cell culture chambers which have different volumes. Furthermore, the present invention provides a suspension cell culture system.

A bio-assembly, a kit for a bio-assembly and methods of using the same are provided. The bio-assembly includes a substrate and a bio-scaffold affixed to the substrate. In accordance with various embodiments, a loader plate having a partition or a loader and a plate having a partition are provided. In accordance with various embodiments, the loader plate includes a partition outlet and a partition inlet, wherein the partition outlet and the partition inlet in fluid communication with the gel. In accordance with various embodiments, the partition comprising an internal volume and shaped to receive the bio-scaffold, and the loader includes a loader inlet and a loader outlet in fluid communication with the gel. In accordance with various embodiments, a bio-compatible adhesive positioned between the substrate and the loader plate or plate. In accordance with various embodiments, a fluid mixture is injected into the bio-scaffold.

Electroporation systems and methods are provided that include a processing assembly including a housing, a lid rotationally connectable to the housing, an opening in a top surface of the housing, an electroporation chamber below the opening in the housing, wherein the electroporation chamber comprises (i) two or more electrodes coated with an electrically conductive, non-cytotoxic material, and (ii) a gasket forming the shape of the electroporation chamber and defining the volume of one or more wells within the electroporation chamber. The system may include a docking station, the docking station comprising, a housing, a port in the housing configured to receive the processing assembly, a lid connected to the housing, one or more contacts configured to connect the docking station to an electroporation system housing.

A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

The invention provides a device for growing cells—referred to as a cassette. The cell culturing device includes a housing that contains a lid having an optically clear window; a fluid distribution channel; a sample injection port fluidically connected to the fluid distribution channel; a base housing a porous media pad; and a media injection port fluidically connected to the media pad. The lid mates to the base to form a sterile seal; the fluid distribution channel is disposed over the media pad, which is viewable through the optical window; and sample fluid introduced into the fluid distribution channel is distributed evenly to the media pad, e.g., via a plurality of channels. The invention also provides kits that include cassettes of the invention and a tube set.

The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

System and method for providing biocompatible, nutrient filled media to the Human Cells, Tissues, and cellular and tissue-based Products (HCT/P) while removing wastes. The present teachings provide for sensing the characteristics of the media, and modifying the characteristics when necessary. The present teachings can also provide components that can provide fluid pumping integrated with fluid gas exchange, and sensing of fluid characteristics at consistent times during the fluid flow cycle. System and method control multiple bioreactors from a centralized media reservoir, while fluidically isolating the bioreactors from cross-contamination.

A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.