A system that includes a cartridge housing and a hollow transfer module, according to an embodiment is described herein. The cartridge housing further includes at least one sample inlet, a plurality of storage chambers, a plurality of reaction chambers, and a fluidic network. The fluidic network is designed to connect the at least one sample inlet, a portion of the plurality of storage chambers and the portion of the plurality of reaction chambers to a first plurality of ports located on an inner surface of the cartridge housing. The hollow transfer module includes a second plurality of ports along an outer surface of the transfer module that lead to a central chamber within the transfer module. The transfer module is designed to move laterally within the cartridge housing. The lateral movement of the transfer module aligns at least a portion of the first plurality of ports with at least a portion of the second plurality of ports.

Provided are multi-layer bioreactors for growing, maintaining, stimulating, monitoring and testing organoids and tissues derived from or representing hollow organs in organoid chambers. Also provided are uses of those bioreactors in modeling a disease process for monitoring disease progress and/or for assessing a biological effect, such as therapeutic efficacy and/or toxicity, e.g., organotoxicity. Also disclosed are bioreactors comprising organoid chambers that are useful as systems for measuring the volume, pressure, contractility, pump function, or electrophysiology of an organoid chamber as well as systems for controlling the pressure experienced by an organoid or tissue in an organoid chamber.

Some fluid coupling devices described herein are configured for use in fluid systems. For example, some embodiments described in this document are single-use, aseptic fluid coupling devices that can be coupled to create a sterile flow path therethrough. Some such aseptic couplings are genderless couplings such that two identical aseptic couplings can be coupled together and then latched to form a robust connection between the aseptic couplings.

The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

A rigid cartridge for housing and removably coupling with a soft body bioreactor is provided. The rigid cartridge includes rigid walls defining a rigid housing. The rigid walls expose a fixed interior configured to house the soft body. An interior surface of a first rigid wall includes a first mating mechanism to engage a second mating mechanism of the soft body to removably coupled with the rigid cartridge and accommodate the soft body in the fixed interior of the rigid cartridge. A rigid wall includes a planar upper surface including an aperture. Each aperture is encompassed by an annular hood protruding upwardly from the substantially planar upper surface and integrally formed with the at least one rigid wall. The aperture is configured to receive and fixed engage a corresponding connector. Each connector includes a rigid coupling mechanism that provides communication with a corresponding plurality of ports of the soft body.

A biological component treatment system includes a biological component kit having tubes that allow a liquid to flow therethrough and a biological component cassette, and a biological component treatment device. The biological component cassette includes flow paths in the interior thereof through which the liquid is allowed to flow, and is equipped with a cassette main body formed in a sheet shape that possesses flexibility. The cassette main body includes a liquid level target detection unit that causes a liquid level of the liquid to be detected. A cross-sectional area of a storage space of the liquid level target detection unit is greater than a cross-sectional area of the flow paths.

A biological component treatment system includes a biological component kit, and a biological component treatment device in which the biological component kit is set. The biological component kit includes tubes and a biological component cassette. The biological component cassette includes a cassette main body having flow paths in the interior of the cassette main body, and the cassette main body includes flow path opening/closing units which are capable of switching between opening and closing of the flow paths. The flow path opening/closing units include cutouts provided at positions adjacent to the flow paths.

There are provided a microscopic mirror imaging device, a system and a method for calibrating a posture of a microneedle. The microscopic mirror imaging device includes a motion actuator, a mirror image former support and a mirror image former. The motion actuator is fixedly mounted on a microscope stage. One end of the mirror image former support is connected to the motion actuator. The mirror image former includes a plane mirror mounted on the other end of the mirror image former support. An angle formed between a mirror surface of the plane mirror and a horizontal plane of the microscope stage is equal to 45°, and an angle formed between the mirror surface of the plane mirror and a coronal plane of the microscope stage is equal to 45°.

The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.

A microplate assembly for a plurality of samples includes a donor microplate having a plurality of sample donor cavities. The microplate assembly further includes a receiver microplate having a plurality of sample receiver cavities each sample receiver cavity having a transparent receiver bottom configured to enable microscopic imaging. In addition, the microplate assembly includes a leak-tight connecting structure configured to assemble the donor microplate and the receiver microplate, with at least one of the sample donor cavities being in communication with at least one of the sample receiver cavities. Further aspects are a receiver microplate and a leak-tight connecting structure for a microplate assembly as well as a method for transferring samples by means of a microplate assembly.