A cell culture apparatus is provided. A cell culture apparatus according to one exemplary embodiment of the present invention comprises: a cell culture part comprising an accommodation space in which a plurality of supports for cell culture are disposed; a medium supply part for storing a predetermined amount of medium to be supplied to the cell culture part, and maintaining a predetermined carbon dioxide concentration of the medium stored therein by using carbon dioxide introduced from the outside through a gas supply port; and a pump, which interconnects the cell culture part and the medium supply part so as to circulate, in the cell culture part, a medium stored in the medium supply part.

A bioreactor (1) for the culture of cells (C) comprising a stack of carriers (7) for cell (C) adherence and liquid medium (M) distribution. The carriers (7) are stacked so as to define levels (6) between adjacent carriers (7) for the flow of the liquid medium (M). Adjacent levels (6) are fluidly interconnected via open spaces (2) so that the liquid medium (M) can flow from one level (6) to an adjacent level (6). The open spaces (2) between a first and an adjacent second level (6′) do not overlap with the one or more open spaces (2) between the second level (6′) and an adjacent third level (6″). One or more of the carriers may also include an area adapted to prevent cell adhesion or growth, thereby allowing for the viewing of cell growth on adjacent carriers from a vantage point external to the bioreactor. Related methods are also disclosed.

The present invention generally relates to a system and process for producing a biosurfactant, and more particularly to a system and process for producing a biosurfactant from at least one strain of Bacillus subtilis, and formulations which comprise the biosurfactant.

The method for analysis and cell culture comprises the following steps: generating a train (14) of ordered drops (16) in a carrier fluid (40), the train (14) of drops (16) comprising at least one culture drop (42), the culture drop (42) comprising a culture medium (50) and at least one cell (4), circulating the train (14) of drops (16) in a tube (10), incubating the train (14) of drops (16) in the tube (10), measuring at least one parameter indicative of the content of the culture drop (42) in the tube (10) at different times, recovering the culture drop (42) at one end (36) of the tube (10),
the steps being carried out in a controlled atmosphere (6).

The present invention relates to a biological (eg cell) processing assembly comprising an aseptic chamber with apertures adapted for gloves to fit and an incubation chamber connectable to aseptic chamber with transfer hatches, doors secured by multi-walls and inflatable seals, stepped collars for tight closure of doors and closed interaction between the chambers. From the aseptic chamber it can be reached through into incubation chamber. There is a docking spigot on outlet wall to interact with stepped collar to define transfer port between the chambers and a disinfectant delivery device to disinfect transfer port.

A substance introduction device according to the present disclosure is a substance introduction device that introduces the substance into the cell by the electroporation, the substance introduction device including: a storage unit that stores the cell suspension containing the cells and the substance; an introduction path that introduces the cell suspension into the storage unit; a filling unit that fills the storage unit with the predetermined amount of the cell suspension from the introduction path; an electrode pair that applies a voltage to the cell suspension stored in the storage unit; and a discharge path that discharges the cell suspension after the voltage is applied from the storage unit.

An apparatus, that relates to the field of in vitro fertilization, is provided for the combined incubation and vitrification of a biological material. The apparatus can be configured to allow for automatic incubation and vitrification of a viable biological material. Thereby predetermined protocols for handling the biological material can be performed precisely and accurately thus avoiding errors and deviations from the intended protocol, as caused by manual human intervention.

There is provided a cell recovery method for recovering cells which are cultured in at least one container containing a liquid medium and adhere to an inner surface of the container, the method including performing: a medium discharge step of discharging the liquid medium from the container; a peeling liquid supply step of supplying a peeling liquid for peeling the cells from the inner surface of the container to the container; a peeling liquid discharge step of discharging the peeling liquid from the container before the cells are completely peeled from the inner surface of the container; a waiting step of waiting until the cells are peeled by action of a residual peeling liquid; and a recovery liquid supply step of supplying a recovery liquid for recovering the cells to the container.

The present invention relates in a first aspect to a plug flow tubular bioreactor having an integral or multi-part tube adapted for cultivation, and, optionally, infection, viral transduction, or transfection of eukaryotic cells. In a further aspect, the present invention relates to a plug flow tubular bioreactor system comprising the plug flow tubular bioreactor according to the present invention. Further, the present invention relates to a method for preparing virus particles, vectors, cells or other molecules including toxic molecules using the plug flow tubular bioreactor or the system according to the present invention. Finally, the present invention relates to the use of a plug flow tubular bioreactor for the preparation of virus particles, vectors including viral vectors, cells including modified cells or other molecules including toxic molecules.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.