Microorganisms present within a plurality of microorganism clusters immobilized in a porous support material may collectively define a supported bio-catalyst. When the microorganisms are effective to convert methane into one or more oxidized carbon compounds (e.g., methanotrophic bacteria), the supported bio-catalysts may be utilized to remove methane from methane-containing gas streams, such as those obtained from mining ventilation. Methods for processing a methane-containing gas stream may comprise interacting the gas stream with the supported bio-catalyst in substantial absence of a liquid phase, and obtaining a methane-depleted gas stream downstream from the supported bio-catalyst. Systems for processing a methane-containing gas stream may comprise the supported bio-catalysts housed in one or more vessels fluidly coupled to a source of methane-containing gas stream. A gas concentration in the methane-containing gas stream and/or the methane-depleted gas stream may be used to determine a current state or anticipated remaining lifetime of the supported bio-catalyst.

A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.

A Cell Migration Assay Plates (CMAP) assembly for high throughput microfluidic migration assays and method of manufacturing thereof are provided. The CMAP assembly includes a top plate having a plurality of wells aligned with a trough component having a plurality of troughs. Each of the wells is defined at least in part by first and second reservoirs and a divisional wall extending between the reservoirs. The trough component is secured to the top plate to form a plurality of micro-channels, such that each one of the micro-channels is defined by a portion of one of the divisional walls and a portion of a corresponding one of the plurality of troughs. The micro-channels enable communication between the reservoirs and visualization of cells migrating through the micro-channels. In this manner, migration of cells through the micro-channels can be visualized for testing and screening applications. A sealing component includes a trough gasket which is operable to be positioned against the bottom end of the well such that the sealing component is sandwiched between the divisional wall and the trough component. The trough gasket is operable to retain the plurality of cells within the troughs such that the plurality of cells migrating towards the second reservoir are isolated within the corresponding trough. At least a portion of the trough component is reconfigurable in relation to the trough gasket and the top plate such that the troughs are exposed to permit a user to retrieve one or more of the cells from the troughs.

A system comprising a collocated thermal plant, water source, CO.sub.2 source and biomass growth module is disclosed. A method of improving the environment by utilizing the system is disclosed.

The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

What is described herein relates to a method for improving a cell culture comprising a) heating a cell culture process fluid using a single-use dense membrane hollow fiber module wherein said single-use dense membrane hollow fiber module comprises a plurality of dense membranes arranged as hollow fibers in a housing and/or b) wherein the cell culture has at least one cell culture chamber and at least the bottom or at least the top of said cell culture chamber is covered with an insulation.

The invention herein describes a novel photobioreactor (PBR) system for culturing algae. The PBR system utilizes vertical transparent tubes that can be cyclically filled with an algae culture. The vertical tubes further feature pipe pigs deployed therein to disrupt formation of biofilm on the inner walls during the cyclic filling and emptying of the cultures.

A cell culture apparatus includes: a cylindrical member that includes a body cylindrical portion and a culture membrane arranged at an opening end portion of the body cylindrical portion; and a cylindrical covering member that is fitted to the body cylindrical portion and includes (i) a first cylindrical portion which covers an outer circumferential surface of the body cylindrical portion when fitted to the cylindrical member and (ii) a second cylindrical portion which is adjacent to the first cylindrical portion in an axial direction of the covering member and forms a container with the culture membrane serving as a bottom surface when fitted to the cylindrical member, and the first cylindrical portion includes a first part in which a length in the axial direction is a first length and a second part in which the length is a second length shorter than the first length.

The present invention relates to a CO2 incubator device and a cloud system connecting the CO2 incubator device with a cloud server. The device includes a plurality of removable incubation unit trays wherein each tray acts as a separate incubator and individual bioreactor. The device includes an inlet valve and an outlet valve for each tray for inflow and outflow of CO2 gas. A microprocessor is coupled to a microcontroller for controlling temperature and CO2 level in the trays based on excess CO2 level present in the trays. A cloud server is connected to the device using a communication protocol for enabling the microprocessor to transmit incubator data to the server and to modify the incubation conditions. The data is stored in a cloud database for enabling users to remotely access the incubator data. The multiple trays are accessed independently enabling multiple users to interact with the incubator.

The present invention relates to systems and methods involving interconnected plate components, including bases, lids and covers, interconnected in a manner such that a continuous strip of each component is prepared. The continuous strips may be stored as rollstock in a reel style. The physical properties of each continuous strip allow the base, lid and/or cover to include means for positive control. In one embodiment, the continuous strip of bases is advanced and processed through an automated system with positive control, and remains in the form of a continuous strip until agar in the bases is cured, at which time the bases are singulated. When a lid is applied to a base using methods described herein, an airtight seal is formed improving the quality of culture media used in testing.