A Petri dish (100) base (300) and lid (200) each having a substantially circular, substantially flat region from which sidewalls extend perpendicular to the flat region (312) forming a corner (326), (230). The base may have a base stacking ring (325) extending from the base (300) at the corner (326), and an internal channel (330) around the inside of the base (300) at the corner (326). The base (300) may have a uniform cross-sectional thickness (350) and may also have vents (322) in the base stacking ring (325). The lid (200) may have a uniform cross-sectional thickness (250). The lid (200) may have a lid stacking ring (225) at the corner (230), which forms a groove (240) in the interior (212) of the lid (200) at the corner (230). The lid may also have ribs (295) and vents (222) to enable multiple dishes to be stacked without forming a vacuum.

An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

Disclosed herein is a bioreactor system that allows active perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprise a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. In some embodiments, application of negative gage pressure to the medium collection chamber or positive pressure to the liquid medium reservoir draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

A modular automated system (10) for sample processing and/or detection of microorganisms is provided. The modular system includes modules (40a, 14, 40b, 50, 55) that perform at least one function in the process of detecting microorganisms in a culture device, such as collection of multiple samples, sample loading, sample preparation, sample incubation, and detection of microorganisms in samples. An exemplary embodiment of a modular sample processing and/or detection system can include an automated loading module, a liquid processing module, modular incubator, a second automated loading module, a reader module, and a collector.

The present disclosure relates to a cell culture apparatus, methods for cell cultivation by using the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus. The cell culture apparatus comprises a plurality of cell culture modules arranged adjacent to each other. Each cell culture module comprises two or more culture medium reservoirs connected by two or more flow channels. The flow channels go through a basal chamber arranged under an apical chamber. The apical and basal chambers are separated by a porous membrane. The bottom part of the at least two culture medium reservoirs comprise a holding member configured to be compatible with a liquid handling system. The basal chamber has a bottom part arranged higher than a bottom part of each of the culture medium reservoirs. The cell culture apparatus further comprises a cavity under the bottom part of the basal chamber.

A cell culture matrix for a perfusion-flow fixed-bed reactor is provided. The cell culture matrix includes a substrate having a porous sheet for adhering cells thereto. The sheet has a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate, arrayed in a regular pattern, and passing through the thickness of the substrate. The porous sheet is wound into a cylindrical shape having a plurality of wound layers, and the cell culture matrix does not include a spacer material or physical barrier between the plurality of wound layers of the substrate.

The present invention is directed to a system, method, use, and diagnostic kit for cell processing. The invention discloses a cell-quantifying component configured for quantifying the cells in the derived tissue. The cell quantifying component can be configured for quantifying the volume flow in the system. Based on the cell-relevant data generated by at least cell quantification, the operational parameters for cell processing are determined.

A bioreactor is provided that permits engineering of multiple different tissues. The bioreactor has a series of flow paths that permit application of tissue-specific media while simultaneously innervating the various different tissues with a common media. The flow paths for the various medias are designed to prevent mixing of the various media as they simultaneously innervate the tissue.

An arrangement (1) for the cultivation of plants and for the utilization of biomass waste and a system (1000) of at least one arrangement (1) for the cultivation and utilization of biomass are disclosed. The arrangement (1) comprises a modular greenhouse (100) and a modular, two-stage biogas plant (200). The system (1000) has at least one arrangement (1) wherein a control and monitoring unit (101) of the modular greenhouse (100) and a further a control and monitoring unit (201) of the two-stage biogas plant (200) are communicatively connected to a central control and monitoring unit (55).