Embodiments relate to a unique design of a cell growth vessel stack system that provides for inter-communication of cell growth levels arranged in a parallel configuration so to facilitate environmental uniformity of the inter-connected growth surface tiers. The unique design also facilitates a fine-tuning of optimized gas and media flow to each cell growth level within the vessel stack. The unique architecture facilitates media refreshment at conveniently scheduled intervals and/or allows constant perfusion from a media reservoir that can be replenished without interrupting the cell proliferation rate. The perfusion rate, nutrient medium condition, CO2 content and osmolality can be controlled to optimize the desired cell proliferation rate, thereby improving cell quantity, quality, and viability.

A microfluidic cell culture device is described. The device comprises a microfluidic network comprising a base, a microfluidic channel, and a cover, and at least one perfusion compartment and at least one support compartment inside the microfluidic channel The base and cover each comprise an aperture, thereby defining a conduit through the microfluidic channel. The aperture is in fluidic contact with the at least one support compartment, and with the at least one perfusion compartment through the at least one support compartment. Methods for creating a fluid-fluid interface and for investigating a cellular response to a stimulant using the device are also described.

A component management apparatus for a bioprocessing system includes a frame having a plurality of segments, including at least a first segment and a second segment pivotably connected to the first segment such that at least the second segment is movable between a closed position and an open position, and at least one mounting bracket connected to the frame for connection of a bioprocess component.

A method for evaluating an adherent cell includes: obtaining an image of an adherent cell accommodated within a culture container; and determining whether the adherent cell is adhering to the culture container based on at least a profile shape of a contact portion of the adherent cell that is in contact with the culture container, the profile shape being specified from the image.

A bioreactor includes a culture medium vessel housing culture medium. The culture medium vessel further includes a first side surface including a first transparent optical window; and a second side surface parallel to the first surface and including one or more sensor adapters to fix optical sensors. A cell retention vessel is disposed underneath and connected to the culture medium vessel, the cell retention vessel housing biological cells and having: a top surface that intersects a base of the culture medium vessel, and a second transparent optical window indented into the top surface at a first corner of the top surface. A semipermeable membrane is disposed at a bottom of the cell retention vessel, and a frame comprising a grid is disposed underneath the semipermeable membrane.

This sample treating device 1000 is provided with: a treating stage 110 for treating a sample by mixing the sample and a plurality of solutions; a first motor 812 for rotating the treating stage 110 around a first rotation shaft 810; a second motor for rotating the treating stage 110 around a second shaft 820; and a control unit for controlling the rotations of the treating stage driven by the first rotary motor 812 and the second motor. The control unit combines the operation of rotating the treating stage 110 around the first rotation shaft 810 and the operation of rotating the treating stage 110 around the second rotation shaft, thereby treating the sample.

The invention relates to a conveying device (IO) for positioning and providing laboratory vessels (12; 12a, 12b, 12c) for nutrient media, samples, microorganisms, cell cultures, or the like for analysis, sample preparation, and/or sample manipulation at an associated apparatus (64), comprising at least one first conveying unit (32) for conveying the laboratory vessels (12) between an initial region (30) and a provision region (60), where the laboratory vessel (12) is held for the analysis or preparation. According to the invention, a plurality of conveying units is present, which perform only a translational motion of the laboratory vessel (12) along an axis, wherein the first conveying unit (32) vertically conveys the laboratory vessel (12) from the initial region (30) to a predetermined height region (50) and vice versa, and a second conveying unit (56) is provided, which horizontally conveys the laboratory vessel (12) from the height region (50) to the provision region and vice versa.

Tissue culture platforms that may be configured for tissue culture or biological cell culture, and methods for use thereof, are described. In general, the tissue culture platforms include multiple well chambers that are fluidically coupled by one or more channels Flow between the different well chambers is controlled via one or more selector valves, enabling a single tissue culture platform that can provide multiple integrated culture subsystems, multiple non-interacting culture subsystems, or combinations thereof.

A system for processing biological material includes a first module and at least a second module, in which each of said first and second modules has at least the following identical characteristics: a size and a shape of a housing; a storage device being rotatably located within said housing; and a plurality of plate slots located in the storage device for receiving plates or containers carrying pipette tips, cell cultures or liquids. The first and second modules are selected from a process module, a storage module, an incubator, a freezer, a plastic-ware store, a refrigerator or a centrifuge.

3D cell cultures and devices for 3D cell culture, and methods of use thereof are provided. In some embodiments, the 3D cell culture comprise pancreatic β cells and can be generated in multi-well plates, allowing for high throughput assays on the cell culture.