The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface in some embodiments. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber in a bioreactor, by controlling the movement of a fluid in which a coating agent is suspended, by changing flow rates, by changing flow directions, by rotation of the bioreactor, and/or combinations thereof.

Provided is a drive mechanism capable of withstanding sterilization treatment using a sterilization gas such as hydrogen peroxide and capable of performing positioning operation with heightened accuracy. In a drive mechanism according to the invention, a movable block disposed in the internal space of a partition wall for blocking a sterilization gas is coupled to a movable platform disposed in the external space of the partition wall by means of a magnet coupling mechanism. Further, biasing magnet units are disposed on surfaces, of the movable block and the movable platform, on which no magnet coupling mechanism is disposed, such that the biasing magnet units serve as magnetic poles repelling each other. Repelling force generated by the biasing magnet units serves as biasing force that biases the movable platform toward the partition wall.

A fluidic cartridge comprises a fluidic disk having a plurality of alignment openings; a fluidic chip comprising a body, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a plurality of protrusions formed on the body and received in the alignment openings of the fluidic disk for aligning the fluidic chip to the fluidic disk; an actuator operably engaging with the one or more channels for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flow rates; and a tube member defining a cylindrical housing for accommodating the fluidic disk, the fluidic chip and the actuator therein.

To continue a series of cell treatments even in a case where a treatment has become impossible in any isolator, a cell treatment system is provided with at least two isolators that perform different treatments, such that a sequential series of cell treatments is performed in each of the isolators. The cell treatment system is provided with a first isolator that performs a first treatment, a second isolator that performs a second treatment different from the first treatment, and a common isolator capable of performing the first treatment and the second treatment.

The invention discloses a first unit (1) for treatment of a bioprocess liquid comprising a first lateral face (2), a second lateral face (3) and a front face (4) which meets the two said lateral faces. The front face comprises: a plurality of valves (7) adapted to receive and act upon one or more legs (8) of a disposable flow path (6); optionally one or more pumps (10) adapted to receive and act upon one or more legs of the disposable flow path; optionally one or more sensors (11) adapted to receive and to measure one or more parameters in one or more legs of the disposable flow path; wherein the plurality of valves and optional pumps and sensors are vertically offset from each other to give one or more legs of a disposable flow path received by said valves and optional pumps and sensors a slope of at least 3.0 degrees from the horizontal plane (h).

The present disclosure provides a fluid delivery consumable for delivering a fluid dose to a bioreactor. The fluid delivery consumable comprising a vial for holding the fluid dose, the vial having an outlet and an open end opposite to the outlet, a plunger engaged with the open end and operable to urge the fluid dose toward the outlet, and a connector proximal to the outlet, the connector being attachable to the bioreactor and adapted to move the fluid dose from the vial to the bioreactor based on operation of the plunger.

A bioprocessing system comprising a series of processing stations for performing operations for bioprocessing is disclosed. The bioprocessing system includes an automated system comprising means for manipulating a fluid connection between a first container and a separable second container whereby to create an aseptic connection that enables a controlled transfer of fluid or cell material between the first container and the second container, wherein the means for manipulating a fluid connection is configured to create an aseptic connection that can be disconnected after the transfer of fluid or cell material is complete to enable a further such fluid connection to be manipulated between the first container and a separable third container, and means for controlling an automated sequence of operation of the processing stations.

A bottle includes a body, a cap mounted to a cap neck of the body. The cap has an outer peripheral portion which is provided with a protrusion. Around the cap, there is provided a position indicator indicating whether the cap is at a correctly closed position, based on the positional relationship between the protrusion and the position indicator.

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower portion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.