The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression and nucleotide production. The invention generally relates to systems and processes that are suitable or comprise a two stage production process, in which the growth of the bacterial host cell is spatiotemporally separated from the production of the protein or nucleic acid of interest. Accordingly, the present invention relates to a system and a process for use in continuous production of a protein of interest or a nucleotide of interest by a bacterial host cell.

A process comprising (i) providing a gaseous stream including greater than 1% by volume carbon dioxide; (ii) providing water; (iii) converting the carbon dioxide and the water to an organic intermediate and oxygen gas in the presence of light; (iv) separating the oxygen gas from the organic intermediate; and (v) converting the organic intermediate to ethylene and carbon dioxide after said step of separating the oxygen gas from the organic intermediate.

An arrangement (1) for the cultivation of plants and for the utilization of biomass waste and a system (1000) of at least one arrangement (1) for the cultivation and utilization of biomass are disclosed. The arrangement (1) comprises a modular greenhouse (100) and a modular, two-stage biogas plant (200). The system (1000) has at least one arrangement (1) wherein a control and monitoring unit (101) of the modular greenhouse (100) and a further a control and monitoring unit (201) of the two-stage biogas plant (200) are communicatively connected to a central control and monitoring unit (55).

A microfluidic device in which microfluidic channels are embedded in a culture medium chamber and have open sides. The microfluidic device is patterned with a fluid moved along a hydrophilic surface due to capillary force, and the fluid may be rapidly and uniformly patterned along an inner corner path and a microfluidic channel. In the microfluidic device, the microfluidic channel is connected to facilitate fluid flow with a culture medium through open sides thereof and openings, and thus may provide a cell culture environment in which high gas saturation is maintained. In addition, several microfluidic devices formed on one common substrate are described. Such microfluidic devices may be manufactured of a hydrophilic engineering plastic by injection molding.

The present invention concerns a method for cleaning a reactor (4) for treating a lignocellulosic biomass (P), said method comprising the following steps: a) draining the reactor of the reaction mixture containing the biomass, b) filling the reactor with a basic aqueous solution (EB), c) draining the reactor, d) injecting steam (V) into the reactor, e) filling the reactor with an aqueous solution (E), f) draining the reactor.

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

Reactor for enzymatic hydrolysis of a raw material comprising in sequence: i)—a first heat exchanger adapted to heat the raw material supplied to the reactor to a temperature within a range that favours enzymatic hydrolysis, ii)—a reactor comprising plural in reactor chambers connected in series, separated by closable valves, iii)—a second heat exchanger adapted to heat the reaction mixture to a temperature higher than the temperature range favouring enzymatic hydrolysis, the reactor being formed with inclined tubular reactor chambers assembled to form a reactor with vertical axis, the first reactor chamber being the vertically uppermost chamber of the reactor, while at least one reactor chamber is adapted to be stirred with a through-flowing inert gas.

A method for the continuous flow synthesis of (R)-4-halo-3-hydroxy-butyrate using a micro-reaction system. The micro-reaction system includes a micro-mixer, a certain number of micro-reaction units that are successively connected in series, a pH regulating system and a back pressure valve. The micro-reaction unit is composed of a micro-channel reactor and a pH regulator that are sequentially connected with each other. A substrate solution containing halogenated acetoacetate and a biocatalyst solution are simultaneously pumped into the micro-reaction system to enable continuous flow biocatalytic asymmetric reduction reaction of the halogenated acetoacetate to obtain the target product (R)-4-halo-3-hydroxy-butyrate.

A continuous flow system for passing fluid over a biofilm to simulate an oral environment is described. In one embodiment, the continuous flow system includes a plurality of channels fluidly connected by one or more channel connectors, an inflow conduit defining an inflow channel and an outflow conduit defining an outflow channel. The plurality of channels can receive the fluid via the inflow conduit from a reservoir positioned upstream of the flow cell housing and the outflow channel can receive the fluid from the plurality of channels.

A production process monitoring system is used to monitor a set of production parameters in a production process of a plurality of production units that perform parallel production operation. The production units comprise at least one reference production unit and at least one distribution production unit. The reference production unit comprises a first sensor, a second sensor, and a reference control unit. The distribution production unit comprises a second sensor and a distribution controller. The first and second sensors of the reference production unit is configured to monitor a first and second parameters in the production parameters in the reference production unit and output a first and second signals corresponding to the first and second parameters. The second sensor of the distribution production unit is configured to monitor the second parameter in the distribution production unit and output a second parameter distribution signal corresponding to the second parameter.