A high throughput microdroplet-based system for single cell assays in microdroplets is provided. The system can include microdroplet sorting. Methods of use of the system are provided.

A microdroplet treatment device, comprising: a sample introduction system, a microfluidic chip system (46), a temperature control system, a droplet recognition system, a droplet detection system and a control system (45); the sample introduction system is used for introducing, into the microdroplet treatment device, samples of aqueous phase and oil phase, and the sample introduction system comprises at least: a sample introduction system I (41) and a sample introduction system II (42), the sample introduction system I (41) being used for introducing, into the microdroplet treatment device, samples of oil phase, and the sample introduction system II (42) being used for introducing into the microdroplet treatment device, samples of aqueous phase; the microfluidic chip system (46) comprises a substrate (4), pipes (1a, 1b, 1c, 1d, 2, 3) formed in the substrate, and a first detection window (9) and a second detection window (10); the temperature control system comprises: a temperature sensor and a temperature control member; the droplet recognition system comprises: a laser light source (55) and a photoelectric sensor (56); the droplet detection system comprises: an optical fiber (57), a spectrometer (58) and a halogen light source (59); and the control system (45) is used for controlling each of the systems in the microdroplet treatment device.

An object of the present invention is to provide a technique for precisely arranging nerve cells on a substrate while suppressing the migration of nerve cells.

A manufacturing method for a substrate on which nerve cells are arranged is provided, the method including a step of arranging, on a substrate, a plurality of liquid droplets containing nerve cells by an inkjet method to form one or a plurality of liquid pools, the substrate having a region in which a cell adhesive material is arranged and a region in which a cell non-adhesive material is arranged; and a step of incubating the liquid pool until the nerve cells sediment and temporarily adhere onto the substrate to form a cell aggregate. The diameter per one liquid pool is 500 μm or less, and the density of nerve cells per one liquid pool is 10.sup.5 cells/cm.sup.2 or more.

Provided is a sample processing method that liquefies a medium solution by making a liquid that liquefies the medium solution act on a sample formed by gelating or solidifying the medium solution that is supported by a substrate while an observation subject is included therein, while maintaining a state in which the medium solution is supported by the substrate while the observation subject is included therein.

A droplet forming device is provided. The droplet forming device includes a liquid holder configured to hold a liquid, a film having a discharge hole, two or more vibration generators configured to vibrate the film, and a driver configured to apply a driving signal to the vibration generators. One or more of the vibration generators are disposed in each region on the film where a polarity of bending moment differs.

The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets.

A microfluidic device provides high throughput generation and analysis of defined three-dimensional cell spheroids with controlled geometry, size, and cell composition. The cell spheroids of the invention mimic tumor microenvironments, including pathophysiological gradients, cell composition, and heterogeneity of the tumor mass mimicking the resistance to drug penetration providing more realistic drug response. The device is used to test the effects of antitumor agents.

A microfluidic chip system for generating droplets is provided by the present disclosure. The microfluidic chip system includes a droplet generation device for generating the droplets, a power generation device for supplying power to the droplet generation device, a collection container for collecting the droplets flowing out of the droplet generation device, a connection device connecting the droplet generation device, the power generation device, and the collection container to each other, and a preparation platform holds together the droplet generation device, the power generation device, and the collection container. A method for preparing the droplets is also provided by the present disclosure.

Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Micro-Organosphers, including Patient-Derived Micro-Organospheres (PMOS s), apparatuses and methods of making them, and apparatuses and methods of using them. Also described herein are methods and systems for screening a patient using these Patient-Derived Micro-Organospheres, including personalized therapies.