A method of lithographic masking for spatially localized biochemical stimulus delivery, comprising the steps of providing a group of cells on a substrate, coating a layer of gelatin on a portion of the cells, creating a mask layer on a portion of the layer of gelatin on a portion of the cells, and creating an area of masked cells and an area of unmasked cells. Further, the method can include delivering a biochemical signal to the area of unmasked cells, removing the mask layer, and allowing the cells with the biochemical signal and the cells without the biochemical signal to interact freely.

A microfluidic structure includes a first media channel or well and a second media channel. A removable membrane is provided between the first media channel or well and the second media channel to permit diffusion. One or more plates is used to form the second media channel. A method of creating a microenvironment using the microfluidic structure is also provided.

Provided is a cell culture sheet. A cell culture sheet according to an embodiment of the present invention includes: a fiber web which has a 3-dimensional network structure formed through the accumulation of support fibers having an average diameter of at most 1.5 μm, and has a basis weight of 1 to 15 g/m.sup.2; and a functional coating layer which is coated on the support fibers exposed on at least one surface of the fiber web, and has a function of promoting one or more of the adhesion, movement, proliferation, and differentiation of cells. Accordingly, cell adhesion is improved due to the large specific surface area and the surface morphology suitable for cells, and the adhered cells are stably supported. Moreover, the cells can be cultured at high density at a high culture efficiency, and can be cultured and recovered without agglomeration caused by forming a thin film.

The present invention relates to a bioreactor for cell culture. According to an embodiment of the present invention, vibration is provided to a cell culture solution (solution) to facilitate cell separation, and, since the vibration is transmitted to the entire cell culture solution without dead zones, cell separation efficiency is excellent.

[Problem] An object of the present invention is to produce cancer cell clusters with intrinsic biological properties as cancer tissues, such as morphological polarity and tissue motion polarity, in vitro. [Solution] The present invention relates to a cell culture substrate including a base material and a biocompatible polymer layer, the substrate including a plurality of rough sections on the surface of the substrate, wherein the rough sections are not covered with the biocompatible polymer layer, have a predetermined surface structure with a predetermined shape, and are disposed at predetermined intervals. With the present invention, it is possible to obtain a live cancer cell aggregate having morphological polarity and tissue motion polarity similar to that observed in vivo, by a very easy operation of culturing cancer cells on a cell culture substrate having a predetermined structure, thereby performing live imaging of microtumors in vitro is enabled, which has been conventionally impossible. Moreover, since such a cancer cell aggregate is considered to reproduce a series of flow of development, proliferation, infiltration, metastasis, and recurrence of cancer in vivo, the cancer cell aggregate can be utilized as a research tool in cancer research, or for screening for an anticancer drug.

Medical instrument includes a substrate, in which cells are in contact with or held on a surface of the substrate, and at least the surface of the substrate which holds the cells is formed of a fluorine-containing cyclic olefin polymer which contains a repeating structure unit represented by Formula (1), ##STR00001##
wherein in Formula (1), at least one of R.sup.1 to R.sup.4 is fluorine, an alkyl with 1 to 10 carbon atoms which contains fluorine, an alkoxy with 1 to 10 carbon atoms which contains fluorine, or an alkoxyalkyl with 2 to 10 carbon atoms which contains fluorine, R.sup.1 to R.sup.4 are selected from hydrogen and certain non-fluorinated groups when R.sup.1 to R.sup.4 do not contain fluorine, R.sup.1 to R.sup.4 may be the same as or different from each other, and R.sup.1 to R.sup.4 may be bonded to each other to form a cyclic structure.

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

A method of growing algae in a cultivation container is disclosed. In some the method may include: circulating, via the cultivation container, in a closed loop, a first predetermined amount of gas mixture comprising a first type of gas and at least one second type of gas, the gas mixture may enter the container via one or more entrance spargers and exit via at least one exit pipe, the first type of gas may contain CO.sub.2 at a known first amount; receiving signal indicative of the amount of CO.sub.2 or H.sub.2S, in the gas mixture; when the signal indicates that the amount of CO.sub.2 drops below a first predetermined level or when the signal indicates that the amount of H.sub.2S rises above a first predetermined level, extracting a second predetermined amount of the gas mixture from the cultivation container: and adding an amount of the first type of gas to the gas mixture, equal to the second predetermined amount.

Provided is a culture vessel for adherent cells that has a large culture portion surface area and ensures easily dissociating and recovering cells without consuming a medium or a dissociation solution more than necessary. The bag-shaped adherent cell culture vessel made of flexible packaging material includes a vessel body portion having a first vessel wall and a second vessel wall and one or more injecting/ejecting ports. The vessel body portion has an intermediate culture wall between the first vessel wall and the second vessel wall. A culture chamber is disposed in each of between the first vessel wall and the intermediate culture wall and between the second vessel wall and the intermediate culture wall, and a flow passage communicating between the respective culture chambers is disposed.